Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer

Transgenic sheep can be used to achieve genetic improvements in breeds and as an important large-animal model for biomedical research. In this study, we generated a TALEN plasmid specific for ovine MSTN and transfected it into fetal fibroblast cells of STH sheep. MSTN biallelic-KO somatic cells were...

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Detalles Bibliográficos
Autores principales: Li, Honghui, Wang, Gui, Hao, Zhiqiang, Zhang, Guozhong, Qing, Yubo, Liu, Shuanghui, Qing, Lili, Pan, Weirong, Chen, Lei, Liu, Guichun, Zhao, Ruoping, Jia, Baoyu, Zeng, Luyao, Guo, Jianxiong, Zhao, Lixiao, Zhao, Heng, Lv, Chaoxiang, Xu, Kaixiang, Cheng, Wenmin, Li, Hushan, Zhao, Hong-Ye, Wang, Wen, Wei, Hong-Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031972/
https://www.ncbi.nlm.nih.gov/pubmed/27654750
http://dx.doi.org/10.1038/srep33675

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