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Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation
Bacterial biofilms are involved in adaptation to complex environments and are responsible for persistent bacterial infections. Biofilm formation is a highly complex process during which multifarious genes work together regularly. In this study, we screened the EZ-Tn5 transposon mutant library to ide...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5032638/ https://www.ncbi.nlm.nih.gov/pubmed/27713736 http://dx.doi.org/10.3389/fmicb.2016.01497 |
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author | Du, Hechao Pang, Maoda Dong, Yuhao Wu, Yafeng Wang, Nannan Liu, Jin Awan, Furqan Lu, Chengping Liu, Yongjie |
author_facet | Du, Hechao Pang, Maoda Dong, Yuhao Wu, Yafeng Wang, Nannan Liu, Jin Awan, Furqan Lu, Chengping Liu, Yongjie |
author_sort | Du, Hechao |
collection | PubMed |
description | Bacterial biofilms are involved in adaptation to complex environments and are responsible for persistent bacterial infections. Biofilm formation is a highly complex process during which multifarious genes work together regularly. In this study, we screened the EZ-Tn5 transposon mutant library to identify genes involved in biofilm formation of Aeromonas hydrophila. A total of 24 biofilm-associated genes were identified, the majority of which encoded proteins related to cell structure, transcription and translation, gene regulation, growth and metabolism. The mutant strain TM90, in which a gene encoding oligopeptidase F (pepF) was disturbed, showed significant upregulation of biofilm formation compared to the parental strain. The TM90 colony phenotype was smaller, more transparent, and splendent. The adhesive ability of TM90 to HEp-2 cells was significantly increased compared with the parental strain. Fifty percent lethal dose (LD(50)) determinations in zebrafish demonstrated that the enhanced-biofilm mutant TM90 was highly attenuated relative to the wild-type strain. In conclusion, the pepF gene is demonstrated for the first time to be a negative factor for biofilm formation and is involved in A. hydrophila pathogenicity. |
format | Online Article Text |
id | pubmed-5032638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50326382016-10-06 Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation Du, Hechao Pang, Maoda Dong, Yuhao Wu, Yafeng Wang, Nannan Liu, Jin Awan, Furqan Lu, Chengping Liu, Yongjie Front Microbiol Microbiology Bacterial biofilms are involved in adaptation to complex environments and are responsible for persistent bacterial infections. Biofilm formation is a highly complex process during which multifarious genes work together regularly. In this study, we screened the EZ-Tn5 transposon mutant library to identify genes involved in biofilm formation of Aeromonas hydrophila. A total of 24 biofilm-associated genes were identified, the majority of which encoded proteins related to cell structure, transcription and translation, gene regulation, growth and metabolism. The mutant strain TM90, in which a gene encoding oligopeptidase F (pepF) was disturbed, showed significant upregulation of biofilm formation compared to the parental strain. The TM90 colony phenotype was smaller, more transparent, and splendent. The adhesive ability of TM90 to HEp-2 cells was significantly increased compared with the parental strain. Fifty percent lethal dose (LD(50)) determinations in zebrafish demonstrated that the enhanced-biofilm mutant TM90 was highly attenuated relative to the wild-type strain. In conclusion, the pepF gene is demonstrated for the first time to be a negative factor for biofilm formation and is involved in A. hydrophila pathogenicity. Frontiers Media S.A. 2016-09-22 /pmc/articles/PMC5032638/ /pubmed/27713736 http://dx.doi.org/10.3389/fmicb.2016.01497 Text en Copyright © 2016 Du, Pang, Dong, Wu, Wang, Liu, Awan, Lu and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Du, Hechao Pang, Maoda Dong, Yuhao Wu, Yafeng Wang, Nannan Liu, Jin Awan, Furqan Lu, Chengping Liu, Yongjie Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation |
title | Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation |
title_full | Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation |
title_fullStr | Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation |
title_full_unstemmed | Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation |
title_short | Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation |
title_sort | identification and characterization of an aeromonas hydrophila oligopeptidase gene pepf negatively related to biofilm formation |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5032638/ https://www.ncbi.nlm.nih.gov/pubmed/27713736 http://dx.doi.org/10.3389/fmicb.2016.01497 |
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