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Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells
The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC sat...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5032673/ https://www.ncbi.nlm.nih.gov/pubmed/27594590 http://dx.doi.org/10.1016/j.stemcr.2016.08.002 |
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author | Naldaiz-Gastesi, Neia Goicoechea, María Alonso-Martín, Sonia Aiastui, Ana López-Mayorga, Macarena García-Belda, Paula Lacalle, Jaione San José, Carlos Araúzo-Bravo, Marcos J. Trouilh, Lidwine Anton-Leberre, Véronique Herrero, Diego Matheu, Ander Bernad, Antonio García-Verdugo, José Manuel Carvajal, Jaime J. Relaix, Frédéric Lopez de Munain, Adolfo García-Parra, Patricia Izeta, Ander |
author_facet | Naldaiz-Gastesi, Neia Goicoechea, María Alonso-Martín, Sonia Aiastui, Ana López-Mayorga, Macarena García-Belda, Paula Lacalle, Jaione San José, Carlos Araúzo-Bravo, Marcos J. Trouilh, Lidwine Anton-Leberre, Véronique Herrero, Diego Matheu, Ander Bernad, Antonio García-Verdugo, José Manuel Carvajal, Jaime J. Relaix, Frédéric Lopez de Munain, Adolfo García-Parra, Patricia Izeta, Ander |
author_sort | Naldaiz-Gastesi, Neia |
collection | PubMed |
description | The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5(+), Pax3/Pax7(+) cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment. |
format | Online Article Text |
id | pubmed-5032673 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-50326732016-09-29 Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells Naldaiz-Gastesi, Neia Goicoechea, María Alonso-Martín, Sonia Aiastui, Ana López-Mayorga, Macarena García-Belda, Paula Lacalle, Jaione San José, Carlos Araúzo-Bravo, Marcos J. Trouilh, Lidwine Anton-Leberre, Véronique Herrero, Diego Matheu, Ander Bernad, Antonio García-Verdugo, José Manuel Carvajal, Jaime J. Relaix, Frédéric Lopez de Munain, Adolfo García-Parra, Patricia Izeta, Ander Stem Cell Reports Article The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5(+), Pax3/Pax7(+) cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment. Elsevier 2016-09-01 /pmc/articles/PMC5032673/ /pubmed/27594590 http://dx.doi.org/10.1016/j.stemcr.2016.08.002 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Naldaiz-Gastesi, Neia Goicoechea, María Alonso-Martín, Sonia Aiastui, Ana López-Mayorga, Macarena García-Belda, Paula Lacalle, Jaione San José, Carlos Araúzo-Bravo, Marcos J. Trouilh, Lidwine Anton-Leberre, Véronique Herrero, Diego Matheu, Ander Bernad, Antonio García-Verdugo, José Manuel Carvajal, Jaime J. Relaix, Frédéric Lopez de Munain, Adolfo García-Parra, Patricia Izeta, Ander Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title | Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_full | Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_fullStr | Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_full_unstemmed | Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_short | Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_sort | identification and characterization of the dermal panniculus carnosus muscle stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5032673/ https://www.ncbi.nlm.nih.gov/pubmed/27594590 http://dx.doi.org/10.1016/j.stemcr.2016.08.002 |
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