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Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells

The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC sat...

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Autores principales: Naldaiz-Gastesi, Neia, Goicoechea, María, Alonso-Martín, Sonia, Aiastui, Ana, López-Mayorga, Macarena, García-Belda, Paula, Lacalle, Jaione, San José, Carlos, Araúzo-Bravo, Marcos J., Trouilh, Lidwine, Anton-Leberre, Véronique, Herrero, Diego, Matheu, Ander, Bernad, Antonio, García-Verdugo, José Manuel, Carvajal, Jaime J., Relaix, Frédéric, Lopez de Munain, Adolfo, García-Parra, Patricia, Izeta, Ander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5032673/
https://www.ncbi.nlm.nih.gov/pubmed/27594590
http://dx.doi.org/10.1016/j.stemcr.2016.08.002
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author Naldaiz-Gastesi, Neia
Goicoechea, María
Alonso-Martín, Sonia
Aiastui, Ana
López-Mayorga, Macarena
García-Belda, Paula
Lacalle, Jaione
San José, Carlos
Araúzo-Bravo, Marcos J.
Trouilh, Lidwine
Anton-Leberre, Véronique
Herrero, Diego
Matheu, Ander
Bernad, Antonio
García-Verdugo, José Manuel
Carvajal, Jaime J.
Relaix, Frédéric
Lopez de Munain, Adolfo
García-Parra, Patricia
Izeta, Ander
author_facet Naldaiz-Gastesi, Neia
Goicoechea, María
Alonso-Martín, Sonia
Aiastui, Ana
López-Mayorga, Macarena
García-Belda, Paula
Lacalle, Jaione
San José, Carlos
Araúzo-Bravo, Marcos J.
Trouilh, Lidwine
Anton-Leberre, Véronique
Herrero, Diego
Matheu, Ander
Bernad, Antonio
García-Verdugo, José Manuel
Carvajal, Jaime J.
Relaix, Frédéric
Lopez de Munain, Adolfo
García-Parra, Patricia
Izeta, Ander
author_sort Naldaiz-Gastesi, Neia
collection PubMed
description The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5(+), Pax3/Pax7(+) cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment.
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spelling pubmed-50326732016-09-29 Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells Naldaiz-Gastesi, Neia Goicoechea, María Alonso-Martín, Sonia Aiastui, Ana López-Mayorga, Macarena García-Belda, Paula Lacalle, Jaione San José, Carlos Araúzo-Bravo, Marcos J. Trouilh, Lidwine Anton-Leberre, Véronique Herrero, Diego Matheu, Ander Bernad, Antonio García-Verdugo, José Manuel Carvajal, Jaime J. Relaix, Frédéric Lopez de Munain, Adolfo García-Parra, Patricia Izeta, Ander Stem Cell Reports Article The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5(+), Pax3/Pax7(+) cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment. Elsevier 2016-09-01 /pmc/articles/PMC5032673/ /pubmed/27594590 http://dx.doi.org/10.1016/j.stemcr.2016.08.002 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Naldaiz-Gastesi, Neia
Goicoechea, María
Alonso-Martín, Sonia
Aiastui, Ana
López-Mayorga, Macarena
García-Belda, Paula
Lacalle, Jaione
San José, Carlos
Araúzo-Bravo, Marcos J.
Trouilh, Lidwine
Anton-Leberre, Véronique
Herrero, Diego
Matheu, Ander
Bernad, Antonio
García-Verdugo, José Manuel
Carvajal, Jaime J.
Relaix, Frédéric
Lopez de Munain, Adolfo
García-Parra, Patricia
Izeta, Ander
Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells
title Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells
title_full Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells
title_fullStr Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells
title_full_unstemmed Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells
title_short Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells
title_sort identification and characterization of the dermal panniculus carnosus muscle stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5032673/
https://www.ncbi.nlm.nih.gov/pubmed/27594590
http://dx.doi.org/10.1016/j.stemcr.2016.08.002
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