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Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance

The pineapple (Ananas comosus) is cold sensitive. Most cultivars are injured during winter periods, especially in sub-tropical regions. There is a lack of molecular information on the pineapple’s response to cold stress. In this study, high-throughput transcriptome sequencing and gene expression ana...

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Autores principales: Chen, Chengjie, Zhang, Yafeng, Xu, Zhiqiang, Luan, Aiping, Mao, Qi, Feng, Junting, Xie, Tao, Gong, Xue, Wang, Xiaoshuang, Chen, Hao, He, Yehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033252/
https://www.ncbi.nlm.nih.gov/pubmed/27656892
http://dx.doi.org/10.1371/journal.pone.0163315
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author Chen, Chengjie
Zhang, Yafeng
Xu, Zhiqiang
Luan, Aiping
Mao, Qi
Feng, Junting
Xie, Tao
Gong, Xue
Wang, Xiaoshuang
Chen, Hao
He, Yehua
author_facet Chen, Chengjie
Zhang, Yafeng
Xu, Zhiqiang
Luan, Aiping
Mao, Qi
Feng, Junting
Xie, Tao
Gong, Xue
Wang, Xiaoshuang
Chen, Hao
He, Yehua
author_sort Chen, Chengjie
collection PubMed
description The pineapple (Ananas comosus) is cold sensitive. Most cultivars are injured during winter periods, especially in sub-tropical regions. There is a lack of molecular information on the pineapple’s response to cold stress. In this study, high-throughput transcriptome sequencing and gene expression analysis were performed on plantlets of a cold-tolerant genotype of the pineapple cultivar ‘Shenwan’ before and after cold treatment. A total of 1,186 candidate cold responsive genes were identified, and their credibility was confirmed by RT-qPCR. Gene set functional enrichment analysis indicated that genes related to cell wall properties, stomatal closure and ABA and ROS signal transduction play important roles in pineapple cold tolerance. In addition, a protein association network of CORs (cold responsive genes) was predicted, which could serve as an entry point to dissect the complex cold response network. Our study found a series of candidate genes and their association network, which will be helpful to cold stress response studies and pineapple breeding for cold tolerance.
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spelling pubmed-50332522016-10-10 Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance Chen, Chengjie Zhang, Yafeng Xu, Zhiqiang Luan, Aiping Mao, Qi Feng, Junting Xie, Tao Gong, Xue Wang, Xiaoshuang Chen, Hao He, Yehua PLoS One Research Article The pineapple (Ananas comosus) is cold sensitive. Most cultivars are injured during winter periods, especially in sub-tropical regions. There is a lack of molecular information on the pineapple’s response to cold stress. In this study, high-throughput transcriptome sequencing and gene expression analysis were performed on plantlets of a cold-tolerant genotype of the pineapple cultivar ‘Shenwan’ before and after cold treatment. A total of 1,186 candidate cold responsive genes were identified, and their credibility was confirmed by RT-qPCR. Gene set functional enrichment analysis indicated that genes related to cell wall properties, stomatal closure and ABA and ROS signal transduction play important roles in pineapple cold tolerance. In addition, a protein association network of CORs (cold responsive genes) was predicted, which could serve as an entry point to dissect the complex cold response network. Our study found a series of candidate genes and their association network, which will be helpful to cold stress response studies and pineapple breeding for cold tolerance. Public Library of Science 2016-09-22 /pmc/articles/PMC5033252/ /pubmed/27656892 http://dx.doi.org/10.1371/journal.pone.0163315 Text en © 2016 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chen, Chengjie
Zhang, Yafeng
Xu, Zhiqiang
Luan, Aiping
Mao, Qi
Feng, Junting
Xie, Tao
Gong, Xue
Wang, Xiaoshuang
Chen, Hao
He, Yehua
Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance
title Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance
title_full Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance
title_fullStr Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance
title_full_unstemmed Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance
title_short Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance
title_sort transcriptome profiling of the pineapple under low temperature to facilitate its breeding for cold tolerance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033252/
https://www.ncbi.nlm.nih.gov/pubmed/27656892
http://dx.doi.org/10.1371/journal.pone.0163315
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