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Development of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener(1)

PREMISE OF THE STUDY: To examine patterns of genetic diversity and test possible hybridization events, microsatellite markers were identified and characterized in Hoya ledongensis (Apocynaceae), and cross-amplification was tested in a congener, H. jianfenglingensis. METHODS AND RESULTS: Based on the...

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Autores principales: Chen, Zheng, Deng, Yuncheng, Zhou, Renchao, He, Shaoyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033368/
https://www.ncbi.nlm.nih.gov/pubmed/27672524
http://dx.doi.org/10.3732/apps.1600059
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author Chen, Zheng
Deng, Yuncheng
Zhou, Renchao
He, Shaoyun
author_facet Chen, Zheng
Deng, Yuncheng
Zhou, Renchao
He, Shaoyun
author_sort Chen, Zheng
collection PubMed
description PREMISE OF THE STUDY: To examine patterns of genetic diversity and test possible hybridization events, microsatellite markers were identified and characterized in Hoya ledongensis (Apocynaceae), and cross-amplification was tested in a congener, H. jianfenglingensis. METHODS AND RESULTS: Based on the transcriptome data of H. ledongensis, 46 microsatellite primer pairs were randomly selected for initial validation. From these, 28 primer pairs were successfully amplified, 12 of which were polymorphic in 36 individuals across three populations of H. ledongensis. The number of alleles per microsatellite locus ranged from two to 11. The observed and expected heterozygosities for the 12 loci ranged from 0.133 to 0.867 and 0.128 to 0.894, respectively. Cross-species amplification was successful for these 12 loci in the congeneric species H. jianfenglingensis. CONCLUSIONS: These polymorphic transcriptome-derived simple sequence repeat markers have the potential to be used as multilocus molecular markers to study the population genetics and natural hybridization in species of Hoya.
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spelling pubmed-50333682016-09-26 Development of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener(1) Chen, Zheng Deng, Yuncheng Zhou, Renchao He, Shaoyun Appl Plant Sci Primer Note PREMISE OF THE STUDY: To examine patterns of genetic diversity and test possible hybridization events, microsatellite markers were identified and characterized in Hoya ledongensis (Apocynaceae), and cross-amplification was tested in a congener, H. jianfenglingensis. METHODS AND RESULTS: Based on the transcriptome data of H. ledongensis, 46 microsatellite primer pairs were randomly selected for initial validation. From these, 28 primer pairs were successfully amplified, 12 of which were polymorphic in 36 individuals across three populations of H. ledongensis. The number of alleles per microsatellite locus ranged from two to 11. The observed and expected heterozygosities for the 12 loci ranged from 0.133 to 0.867 and 0.128 to 0.894, respectively. Cross-species amplification was successful for these 12 loci in the congeneric species H. jianfenglingensis. CONCLUSIONS: These polymorphic transcriptome-derived simple sequence repeat markers have the potential to be used as multilocus molecular markers to study the population genetics and natural hybridization in species of Hoya. Botanical Society of America 2016-09-19 /pmc/articles/PMC5033368/ /pubmed/27672524 http://dx.doi.org/10.3732/apps.1600059 Text en © 2016 Chen et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Chen, Zheng
Deng, Yuncheng
Zhou, Renchao
He, Shaoyun
Development of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener(1)
title Development of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener(1)
title_full Development of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener(1)
title_fullStr Development of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener(1)
title_full_unstemmed Development of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener(1)
title_short Development of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener(1)
title_sort development of transcriptome-derived ssr markers for hoya ledongensis (apocynaceae) and cross-amplification in a congener(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033368/
https://www.ncbi.nlm.nih.gov/pubmed/27672524
http://dx.doi.org/10.3732/apps.1600059
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