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Identification of Specific Effect of Chloride on the Spectral Properties and Structural Stability of Multiple Extracellular Glutamic Acid Mutants of Bacteriorhodopsin

In the present work we combine spectroscopic, DSC and computational approaches to examine the multiple extracellular Glu mutants E204Q/E194Q, E204Q/E194Q/E9Q and E204Q/E194Q/E9Q/E74Q of bacteriorhodopsin by varying solvent ionic strength and composition. Absorption spectroscopy data reveal that the...

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Detalles Bibliográficos
Autores principales:	Lazarova, Tzvetana, Mlynarczyk, Krzysztof, Querol, Enric, Tenchov, Boris, Filipek, Slawomir, Padrós, Esteve
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033488/ https://www.ncbi.nlm.nih.gov/pubmed/27657718 http://dx.doi.org/10.1371/journal.pone.0162952

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