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Genome-wide mapping of nucleosome positions in Saccharomyces cerevisiae in response to different nitrogen conditions

Well-organized chromatin is involved in a number of various transcriptional regulation and gene expression. We used genome-wide mapping of nucleosomes in response to different nitrogen conditions to determine both nucleosome profiles and gene expression events in Saccharomyces cerevisiae. Nitrogen c...

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Autores principales: Zhang, Peng, Du, Guocheng, Zou, Huijun, Xie, Guangfa, Chen, Jian, Shi, Zhongping, Zhou, Jingwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034280/
https://www.ncbi.nlm.nih.gov/pubmed/27659668
http://dx.doi.org/10.1038/srep33970
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author Zhang, Peng
Du, Guocheng
Zou, Huijun
Xie, Guangfa
Chen, Jian
Shi, Zhongping
Zhou, Jingwen
author_facet Zhang, Peng
Du, Guocheng
Zou, Huijun
Xie, Guangfa
Chen, Jian
Shi, Zhongping
Zhou, Jingwen
author_sort Zhang, Peng
collection PubMed
description Well-organized chromatin is involved in a number of various transcriptional regulation and gene expression. We used genome-wide mapping of nucleosomes in response to different nitrogen conditions to determine both nucleosome profiles and gene expression events in Saccharomyces cerevisiae. Nitrogen conditions influence general nucleosome profiles and the expression of nitrogen catabolite repression (NCR) sensitive genes. The nucleosome occupancy of TATA-containing genes was higher compared to TATA-less genes. TATA-less genes in high or low nucleosome occupancy, showed a significant change in gene coding regions when shifting cells from glutamine to proline as the sole nitrogen resource. Furthermore, a correlation between the expression of nucleosome occupancy induced NCR sensitive genes or TATA containing genes in NCR sensitive genes, and nucleosome prediction were found when cells were cultured in proline or shifting from glutamine to proline as the sole nitrogen source compared to glutamine. These results also showed that variation of nucleosome occupancy accompany with chromatin-dependent transcription factor could influence the expression of a series of genes involved in the specific regulation of nitrogen utilization.
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spelling pubmed-50342802016-09-29 Genome-wide mapping of nucleosome positions in Saccharomyces cerevisiae in response to different nitrogen conditions Zhang, Peng Du, Guocheng Zou, Huijun Xie, Guangfa Chen, Jian Shi, Zhongping Zhou, Jingwen Sci Rep Article Well-organized chromatin is involved in a number of various transcriptional regulation and gene expression. We used genome-wide mapping of nucleosomes in response to different nitrogen conditions to determine both nucleosome profiles and gene expression events in Saccharomyces cerevisiae. Nitrogen conditions influence general nucleosome profiles and the expression of nitrogen catabolite repression (NCR) sensitive genes. The nucleosome occupancy of TATA-containing genes was higher compared to TATA-less genes. TATA-less genes in high or low nucleosome occupancy, showed a significant change in gene coding regions when shifting cells from glutamine to proline as the sole nitrogen resource. Furthermore, a correlation between the expression of nucleosome occupancy induced NCR sensitive genes or TATA containing genes in NCR sensitive genes, and nucleosome prediction were found when cells were cultured in proline or shifting from glutamine to proline as the sole nitrogen source compared to glutamine. These results also showed that variation of nucleosome occupancy accompany with chromatin-dependent transcription factor could influence the expression of a series of genes involved in the specific regulation of nitrogen utilization. Nature Publishing Group 2016-09-23 /pmc/articles/PMC5034280/ /pubmed/27659668 http://dx.doi.org/10.1038/srep33970 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhang, Peng
Du, Guocheng
Zou, Huijun
Xie, Guangfa
Chen, Jian
Shi, Zhongping
Zhou, Jingwen
Genome-wide mapping of nucleosome positions in Saccharomyces cerevisiae in response to different nitrogen conditions
title Genome-wide mapping of nucleosome positions in Saccharomyces cerevisiae in response to different nitrogen conditions
title_full Genome-wide mapping of nucleosome positions in Saccharomyces cerevisiae in response to different nitrogen conditions
title_fullStr Genome-wide mapping of nucleosome positions in Saccharomyces cerevisiae in response to different nitrogen conditions
title_full_unstemmed Genome-wide mapping of nucleosome positions in Saccharomyces cerevisiae in response to different nitrogen conditions
title_short Genome-wide mapping of nucleosome positions in Saccharomyces cerevisiae in response to different nitrogen conditions
title_sort genome-wide mapping of nucleosome positions in saccharomyces cerevisiae in response to different nitrogen conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034280/
https://www.ncbi.nlm.nih.gov/pubmed/27659668
http://dx.doi.org/10.1038/srep33970
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