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Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein

Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one...

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Autores principales: Hashimoto, Takuma, Horikawa, Daiki D., Saito, Yuki, Kuwahara, Hirokazu, Kozuka-Hata, Hiroko, Shin-I, Tadasu, Minakuchi, Yohei, Ohishi, Kazuko, Motoyama, Ayuko, Aizu, Tomoyuki, Enomoto, Atsushi, Kondo, Koyuki, Tanaka, Sae, Hara, Yuichiro, Koshikawa, Shigeyuki, Sagara, Hiroshi, Miura, Toru, Yokobori, Shin-ichi, Miyagawa, Kiyoshi, Suzuki, Yutaka, Kubo, Takeo, Oyama, Masaaki, Kohara, Yuji, Fujiyama, Asao, Arakawa, Kazuharu, Katayama, Toshiaki, Toyoda, Atsushi, Kunieda, Takekazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034306/
https://www.ncbi.nlm.nih.gov/pubmed/27649274
http://dx.doi.org/10.1038/ncomms12808
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author Hashimoto, Takuma
Horikawa, Daiki D.
Saito, Yuki
Kuwahara, Hirokazu
Kozuka-Hata, Hiroko
Shin-I, Tadasu
Minakuchi, Yohei
Ohishi, Kazuko
Motoyama, Ayuko
Aizu, Tomoyuki
Enomoto, Atsushi
Kondo, Koyuki
Tanaka, Sae
Hara, Yuichiro
Koshikawa, Shigeyuki
Sagara, Hiroshi
Miura, Toru
Yokobori, Shin-ichi
Miyagawa, Kiyoshi
Suzuki, Yutaka
Kubo, Takeo
Oyama, Masaaki
Kohara, Yuji
Fujiyama, Asao
Arakawa, Kazuharu
Katayama, Toshiaki
Toyoda, Atsushi
Kunieda, Takekazu
author_facet Hashimoto, Takuma
Horikawa, Daiki D.
Saito, Yuki
Kuwahara, Hirokazu
Kozuka-Hata, Hiroko
Shin-I, Tadasu
Minakuchi, Yohei
Ohishi, Kazuko
Motoyama, Ayuko
Aizu, Tomoyuki
Enomoto, Atsushi
Kondo, Koyuki
Tanaka, Sae
Hara, Yuichiro
Koshikawa, Shigeyuki
Sagara, Hiroshi
Miura, Toru
Yokobori, Shin-ichi
Miyagawa, Kiyoshi
Suzuki, Yutaka
Kubo, Takeo
Oyama, Masaaki
Kohara, Yuji
Fujiyama, Asao
Arakawa, Kazuharu
Katayama, Toshiaki
Toyoda, Atsushi
Kunieda, Takekazu
author_sort Hashimoto, Takuma
collection PubMed
description Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one of the most stress-tolerant tardigrade species. Precise gene repertoire analyses reveal the presence of a small proportion (1.2% or less) of putative foreign genes, loss of gene pathways that promote stress damage, expansion of gene families related to ameliorating damage, and evolution and high expression of novel tardigrade-unique proteins. Minor changes in the gene expression profiles during dehydration and rehydration suggest constitutive expression of tolerance-related genes. Using human cultured cells, we demonstrate that a tardigrade-unique DNA-associating protein suppresses X-ray-induced DNA damage by ∼40% and improves radiotolerance. These findings indicate the relevance of tardigrade-unique proteins to tolerability and tardigrades could be a bountiful source of new protection genes and mechanisms.
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spelling pubmed-50343062016-10-04 Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein Hashimoto, Takuma Horikawa, Daiki D. Saito, Yuki Kuwahara, Hirokazu Kozuka-Hata, Hiroko Shin-I, Tadasu Minakuchi, Yohei Ohishi, Kazuko Motoyama, Ayuko Aizu, Tomoyuki Enomoto, Atsushi Kondo, Koyuki Tanaka, Sae Hara, Yuichiro Koshikawa, Shigeyuki Sagara, Hiroshi Miura, Toru Yokobori, Shin-ichi Miyagawa, Kiyoshi Suzuki, Yutaka Kubo, Takeo Oyama, Masaaki Kohara, Yuji Fujiyama, Asao Arakawa, Kazuharu Katayama, Toshiaki Toyoda, Atsushi Kunieda, Takekazu Nat Commun Article Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one of the most stress-tolerant tardigrade species. Precise gene repertoire analyses reveal the presence of a small proportion (1.2% or less) of putative foreign genes, loss of gene pathways that promote stress damage, expansion of gene families related to ameliorating damage, and evolution and high expression of novel tardigrade-unique proteins. Minor changes in the gene expression profiles during dehydration and rehydration suggest constitutive expression of tolerance-related genes. Using human cultured cells, we demonstrate that a tardigrade-unique DNA-associating protein suppresses X-ray-induced DNA damage by ∼40% and improves radiotolerance. These findings indicate the relevance of tardigrade-unique proteins to tolerability and tardigrades could be a bountiful source of new protection genes and mechanisms. Nature Publishing Group 2016-09-20 /pmc/articles/PMC5034306/ /pubmed/27649274 http://dx.doi.org/10.1038/ncomms12808 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Hashimoto, Takuma
Horikawa, Daiki D.
Saito, Yuki
Kuwahara, Hirokazu
Kozuka-Hata, Hiroko
Shin-I, Tadasu
Minakuchi, Yohei
Ohishi, Kazuko
Motoyama, Ayuko
Aizu, Tomoyuki
Enomoto, Atsushi
Kondo, Koyuki
Tanaka, Sae
Hara, Yuichiro
Koshikawa, Shigeyuki
Sagara, Hiroshi
Miura, Toru
Yokobori, Shin-ichi
Miyagawa, Kiyoshi
Suzuki, Yutaka
Kubo, Takeo
Oyama, Masaaki
Kohara, Yuji
Fujiyama, Asao
Arakawa, Kazuharu
Katayama, Toshiaki
Toyoda, Atsushi
Kunieda, Takekazu
Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
title Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
title_full Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
title_fullStr Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
title_full_unstemmed Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
title_short Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
title_sort extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034306/
https://www.ncbi.nlm.nih.gov/pubmed/27649274
http://dx.doi.org/10.1038/ncomms12808
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