Cargando…
Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein
Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034306/ https://www.ncbi.nlm.nih.gov/pubmed/27649274 http://dx.doi.org/10.1038/ncomms12808 |
_version_ | 1782455244173606912 |
---|---|
author | Hashimoto, Takuma Horikawa, Daiki D. Saito, Yuki Kuwahara, Hirokazu Kozuka-Hata, Hiroko Shin-I, Tadasu Minakuchi, Yohei Ohishi, Kazuko Motoyama, Ayuko Aizu, Tomoyuki Enomoto, Atsushi Kondo, Koyuki Tanaka, Sae Hara, Yuichiro Koshikawa, Shigeyuki Sagara, Hiroshi Miura, Toru Yokobori, Shin-ichi Miyagawa, Kiyoshi Suzuki, Yutaka Kubo, Takeo Oyama, Masaaki Kohara, Yuji Fujiyama, Asao Arakawa, Kazuharu Katayama, Toshiaki Toyoda, Atsushi Kunieda, Takekazu |
author_facet | Hashimoto, Takuma Horikawa, Daiki D. Saito, Yuki Kuwahara, Hirokazu Kozuka-Hata, Hiroko Shin-I, Tadasu Minakuchi, Yohei Ohishi, Kazuko Motoyama, Ayuko Aizu, Tomoyuki Enomoto, Atsushi Kondo, Koyuki Tanaka, Sae Hara, Yuichiro Koshikawa, Shigeyuki Sagara, Hiroshi Miura, Toru Yokobori, Shin-ichi Miyagawa, Kiyoshi Suzuki, Yutaka Kubo, Takeo Oyama, Masaaki Kohara, Yuji Fujiyama, Asao Arakawa, Kazuharu Katayama, Toshiaki Toyoda, Atsushi Kunieda, Takekazu |
author_sort | Hashimoto, Takuma |
collection | PubMed |
description | Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one of the most stress-tolerant tardigrade species. Precise gene repertoire analyses reveal the presence of a small proportion (1.2% or less) of putative foreign genes, loss of gene pathways that promote stress damage, expansion of gene families related to ameliorating damage, and evolution and high expression of novel tardigrade-unique proteins. Minor changes in the gene expression profiles during dehydration and rehydration suggest constitutive expression of tolerance-related genes. Using human cultured cells, we demonstrate that a tardigrade-unique DNA-associating protein suppresses X-ray-induced DNA damage by ∼40% and improves radiotolerance. These findings indicate the relevance of tardigrade-unique proteins to tolerability and tardigrades could be a bountiful source of new protection genes and mechanisms. |
format | Online Article Text |
id | pubmed-5034306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50343062016-10-04 Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein Hashimoto, Takuma Horikawa, Daiki D. Saito, Yuki Kuwahara, Hirokazu Kozuka-Hata, Hiroko Shin-I, Tadasu Minakuchi, Yohei Ohishi, Kazuko Motoyama, Ayuko Aizu, Tomoyuki Enomoto, Atsushi Kondo, Koyuki Tanaka, Sae Hara, Yuichiro Koshikawa, Shigeyuki Sagara, Hiroshi Miura, Toru Yokobori, Shin-ichi Miyagawa, Kiyoshi Suzuki, Yutaka Kubo, Takeo Oyama, Masaaki Kohara, Yuji Fujiyama, Asao Arakawa, Kazuharu Katayama, Toshiaki Toyoda, Atsushi Kunieda, Takekazu Nat Commun Article Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one of the most stress-tolerant tardigrade species. Precise gene repertoire analyses reveal the presence of a small proportion (1.2% or less) of putative foreign genes, loss of gene pathways that promote stress damage, expansion of gene families related to ameliorating damage, and evolution and high expression of novel tardigrade-unique proteins. Minor changes in the gene expression profiles during dehydration and rehydration suggest constitutive expression of tolerance-related genes. Using human cultured cells, we demonstrate that a tardigrade-unique DNA-associating protein suppresses X-ray-induced DNA damage by ∼40% and improves radiotolerance. These findings indicate the relevance of tardigrade-unique proteins to tolerability and tardigrades could be a bountiful source of new protection genes and mechanisms. Nature Publishing Group 2016-09-20 /pmc/articles/PMC5034306/ /pubmed/27649274 http://dx.doi.org/10.1038/ncomms12808 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Article Hashimoto, Takuma Horikawa, Daiki D. Saito, Yuki Kuwahara, Hirokazu Kozuka-Hata, Hiroko Shin-I, Tadasu Minakuchi, Yohei Ohishi, Kazuko Motoyama, Ayuko Aizu, Tomoyuki Enomoto, Atsushi Kondo, Koyuki Tanaka, Sae Hara, Yuichiro Koshikawa, Shigeyuki Sagara, Hiroshi Miura, Toru Yokobori, Shin-ichi Miyagawa, Kiyoshi Suzuki, Yutaka Kubo, Takeo Oyama, Masaaki Kohara, Yuji Fujiyama, Asao Arakawa, Kazuharu Katayama, Toshiaki Toyoda, Atsushi Kunieda, Takekazu Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein |
title | Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein |
title_full | Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein |
title_fullStr | Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein |
title_full_unstemmed | Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein |
title_short | Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein |
title_sort | extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034306/ https://www.ncbi.nlm.nih.gov/pubmed/27649274 http://dx.doi.org/10.1038/ncomms12808 |
work_keys_str_mv | AT hashimototakuma extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT horikawadaikid extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT saitoyuki extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT kuwaharahirokazu extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT kozukahatahiroko extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT shinitadasu extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT minakuchiyohei extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT ohishikazuko extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT motoyamaayuko extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT aizutomoyuki extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT enomotoatsushi extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT kondokoyuki extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT tanakasae extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT harayuichiro extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT koshikawashigeyuki extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT sagarahiroshi extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT miuratoru extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT yokoborishinichi extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT miyagawakiyoshi extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT suzukiyutaka extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT kubotakeo extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT oyamamasaaki extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT koharayuji extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT fujiyamaasao extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT arakawakazuharu extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT katayamatoshiaki extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT toyodaatsushi extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein AT kuniedatakekazu extremotoleranttardigradegenomeandimprovedradiotoleranceofhumanculturedcellsbytardigradeuniqueprotein |