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Antibacterial in vitro effects of preparations from Anthroposophical Medicine

BACKGROUND: Medications from Anthroposophical Medicine (AM) are clinically used for the treatment of infections within a whole medical system but have not yet been evaluated regarding antibacterial effects. The aims of this study was to investigate antibacterial activity of AM medications in cell cu...

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Autores principales: Roser, Eva, Gründemann, Carsten, Engels, Inge, Huber, Roman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034436/
https://www.ncbi.nlm.nih.gov/pubmed/27660088
http://dx.doi.org/10.1186/s12906-016-1350-3
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author Roser, Eva
Gründemann, Carsten
Engels, Inge
Huber, Roman
author_facet Roser, Eva
Gründemann, Carsten
Engels, Inge
Huber, Roman
author_sort Roser, Eva
collection PubMed
description BACKGROUND: Medications from Anthroposophical Medicine (AM) are clinically used for the treatment of infections within a whole medical system but have not yet been evaluated regarding antibacterial effects. The aims of this study was to investigate antibacterial activity of AM medications in cell culture. METHODS: Screening of AM drug registers for preparations used to treat any kind of infection and being available in dilutions ≤ D2 and without alcoholic content. Selected medications were screened for antimicrobial activity against Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa using the agar diffusion method. For antimicrobial active preparations growth kinetics (drop plate method) and minimal inhibitory concentrations (MIC, macrodilution method) were determined. RESULTS: Thirty-three preparations matched the selection criteria and were chosen for own experiments. One of them (Berberis Decoctum D2) exhibited bactericidal activities against Bacillus subtilis and Staphylococcus aureus, including methicillin resistant strains. The MIC could be determined as 5 mg/ml. The effects could be related to the content of berberine in the extract. No activity towards gram-negative bacteria was found. The other tested extracts had no antibacterial effects. CONCLUSION: Berberis Decoctum D2 which is used in AM to treat infections exhibits bactericidal effects on Staphylococcus aureus, including methicillin resistant strains.
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spelling pubmed-50344362016-09-29 Antibacterial in vitro effects of preparations from Anthroposophical Medicine Roser, Eva Gründemann, Carsten Engels, Inge Huber, Roman BMC Complement Altern Med Research Article BACKGROUND: Medications from Anthroposophical Medicine (AM) are clinically used for the treatment of infections within a whole medical system but have not yet been evaluated regarding antibacterial effects. The aims of this study was to investigate antibacterial activity of AM medications in cell culture. METHODS: Screening of AM drug registers for preparations used to treat any kind of infection and being available in dilutions ≤ D2 and without alcoholic content. Selected medications were screened for antimicrobial activity against Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa using the agar diffusion method. For antimicrobial active preparations growth kinetics (drop plate method) and minimal inhibitory concentrations (MIC, macrodilution method) were determined. RESULTS: Thirty-three preparations matched the selection criteria and were chosen for own experiments. One of them (Berberis Decoctum D2) exhibited bactericidal activities against Bacillus subtilis and Staphylococcus aureus, including methicillin resistant strains. The MIC could be determined as 5 mg/ml. The effects could be related to the content of berberine in the extract. No activity towards gram-negative bacteria was found. The other tested extracts had no antibacterial effects. CONCLUSION: Berberis Decoctum D2 which is used in AM to treat infections exhibits bactericidal effects on Staphylococcus aureus, including methicillin resistant strains. BioMed Central 2016-09-22 /pmc/articles/PMC5034436/ /pubmed/27660088 http://dx.doi.org/10.1186/s12906-016-1350-3 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Roser, Eva
Gründemann, Carsten
Engels, Inge
Huber, Roman
Antibacterial in vitro effects of preparations from Anthroposophical Medicine
title Antibacterial in vitro effects of preparations from Anthroposophical Medicine
title_full Antibacterial in vitro effects of preparations from Anthroposophical Medicine
title_fullStr Antibacterial in vitro effects of preparations from Anthroposophical Medicine
title_full_unstemmed Antibacterial in vitro effects of preparations from Anthroposophical Medicine
title_short Antibacterial in vitro effects of preparations from Anthroposophical Medicine
title_sort antibacterial in vitro effects of preparations from anthroposophical medicine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034436/
https://www.ncbi.nlm.nih.gov/pubmed/27660088
http://dx.doi.org/10.1186/s12906-016-1350-3
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