Cargando…

Goat K(222)-PrP(C) polymorphic variant does not provide resistance to atypical scrapie in transgenic mice

Host prion (PrP(C)) genotype is a major determinant for the susceptibility to prion diseases. The Q/K(222)-PrP(C) polymorphic variant provides goats and mice with high resistance against classical scrapie and bovine spongiform encephalopathy (BSE); yet its effect against atypical scrapie is unknown....

Descripción completa

Detalles Bibliográficos
Autores principales: Aguilar-Calvo, Patricia, Espinosa, Juan-Carlos, Andréoletti, Olivier, González, Lorenzo, Orge, Leonor, Juste, Ramón, Torres, Juan-María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034450/
https://www.ncbi.nlm.nih.gov/pubmed/27659200
http://dx.doi.org/10.1186/s13567-016-0380-7
Descripción
Sumario:Host prion (PrP(C)) genotype is a major determinant for the susceptibility to prion diseases. The Q/K(222)-PrP(C) polymorphic variant provides goats and mice with high resistance against classical scrapie and bovine spongiform encephalopathy (BSE); yet its effect against atypical scrapie is unknown. Here, transgenic mice expressing the goat wild-type (wt) or the K(222)-PrP(C) variant were intracerebrally inoculated with several natural cases of atypical scrapie from sheep and goat and their susceptibility to the prion disease was determined. Goat wt and K(222)-PrP(C) transgenic mice were 100% susceptible to all the atypical scrapie isolates, showing similar survival times and almost identical disease phenotypes. The capacity of the K(222)-PrP(C) variant to replicate specifically the atypical scrapie strain as efficiently as the goat wt PrP(C), but not the classical scrapie or cattle-BSE as previously reported, further suggests the involvement of concrete areas of the host PrP(C) in the strain-dependent replication of prions.