Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis

The activity of cysteine cathepsin B increased markedly in lung homogenates and in bronchoalveolar lavage fluids (BALF) of the mouse model of bleomycin-induced lung fibrosis after 14 days of challenge. In contrast the level of the cysteine cathepsin inhibitor cystatin C was unaffected in BALF of wil...

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Autores principales: Kasabova, Mariana, Villeret, Bérengère, Gombault, Aurélie, Lecaille, Fabien, Reinheckel, Thomas, Marchand-Adam, Sylvain, Couillin, Isabelle, Lalmanach, Gilles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034538/
https://www.ncbi.nlm.nih.gov/pubmed/27658724
http://dx.doi.org/10.1186/s12931-016-0432-6
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author Kasabova, Mariana
Villeret, Bérengère
Gombault, Aurélie
Lecaille, Fabien
Reinheckel, Thomas
Marchand-Adam, Sylvain
Couillin, Isabelle
Lalmanach, Gilles
author_facet Kasabova, Mariana
Villeret, Bérengère
Gombault, Aurélie
Lecaille, Fabien
Reinheckel, Thomas
Marchand-Adam, Sylvain
Couillin, Isabelle
Lalmanach, Gilles
author_sort Kasabova, Mariana
collection PubMed
description The activity of cysteine cathepsin B increased markedly in lung homogenates and in bronchoalveolar lavage fluids (BALF) of the mouse model of bleomycin-induced lung fibrosis after 14 days of challenge. In contrast the level of the cysteine cathepsin inhibitor cystatin C was unaffected in BALF of wild-type and cathepsin B-deficient mice. Therefore, murine cystatin C is not a reliable marker of fibrosis during bleomycin-induced lung fibrosis. Current data are in sharp contrast to previous analysis carried on human BALF from patients with idiopathic pulmonary fibrosis, for which the level of cathepsin B remained unchanged while cystatin C was significantly increased.
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spelling pubmed-50345382016-09-29 Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis Kasabova, Mariana Villeret, Bérengère Gombault, Aurélie Lecaille, Fabien Reinheckel, Thomas Marchand-Adam, Sylvain Couillin, Isabelle Lalmanach, Gilles Respir Res Letter to the Editor The activity of cysteine cathepsin B increased markedly in lung homogenates and in bronchoalveolar lavage fluids (BALF) of the mouse model of bleomycin-induced lung fibrosis after 14 days of challenge. In contrast the level of the cysteine cathepsin inhibitor cystatin C was unaffected in BALF of wild-type and cathepsin B-deficient mice. Therefore, murine cystatin C is not a reliable marker of fibrosis during bleomycin-induced lung fibrosis. Current data are in sharp contrast to previous analysis carried on human BALF from patients with idiopathic pulmonary fibrosis, for which the level of cathepsin B remained unchanged while cystatin C was significantly increased. BioMed Central 2016-09-22 2016 /pmc/articles/PMC5034538/ /pubmed/27658724 http://dx.doi.org/10.1186/s12931-016-0432-6 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Letter to the Editor
Kasabova, Mariana
Villeret, Bérengère
Gombault, Aurélie
Lecaille, Fabien
Reinheckel, Thomas
Marchand-Adam, Sylvain
Couillin, Isabelle
Lalmanach, Gilles
Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis
title Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis
title_full Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis
title_fullStr Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis
title_full_unstemmed Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis
title_short Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis
title_sort discordance in cathepsin b and cystatin c expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034538/
https://www.ncbi.nlm.nih.gov/pubmed/27658724
http://dx.doi.org/10.1186/s12931-016-0432-6
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