Cargando…

Rare subtype(s) and frequency of multi-viral subtype high-risk human papilloma virus infection in high-grade squamous intraepithelial lesion and squamous cell carcinoma in a cervical carcinoma prevalent developing country: a multiplex real-time polymerase chain reaction-based pilot study

BACKGROUND: Bangladesh, with a population of 160 million and nearly half being women, has the 4th highest rate of cervical carcinoma deaths in the world. It is projected that ∼500,000 of these women would die of this entirely preventable cancer by 2030. HPV vaccination is not widely offered in Bangl...

Descripción completa

Detalles Bibliográficos
Autores principales: Akhter, Shabnam, Manna, Pradip, Kamal, Mohammed, Sung, C. James, Lawrence, W. Dwayne, Quddus, M. Ruhul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034567/
https://www.ncbi.nlm.nih.gov/pubmed/27659129
http://dx.doi.org/10.1186/s13000-016-0542-y
_version_ 1782455296599261184
author Akhter, Shabnam
Manna, Pradip
Kamal, Mohammed
Sung, C. James
Lawrence, W. Dwayne
Quddus, M. Ruhul
author_facet Akhter, Shabnam
Manna, Pradip
Kamal, Mohammed
Sung, C. James
Lawrence, W. Dwayne
Quddus, M. Ruhul
author_sort Akhter, Shabnam
collection PubMed
description BACKGROUND: Bangladesh, with a population of 160 million and nearly half being women, has the 4th highest rate of cervical carcinoma deaths in the world. It is projected that ∼500,000 of these women would die of this entirely preventable cancer by 2030. HPV vaccination is not widely offered in Bangladesh. This pilot study is designed to find out the prevalence of rare and multi-viral high-risk HPV (hrHPV) subtype(s) infection which may help strategize a large scale vaccination program in tackling cervical carcinoma in the country. METHODS: Forty cases of cervical High-Grade squamous intraepithelial lesion (HSIL) and Squamous cell carcinoma (SqCa) were collected. DNA was extracted from tissue representing HSIL and SqCa and multiplex PCR was run to identify all 15 hrHPV subtypes along with known positive controls. RESULTS: Of the total, 27 cases were biopsies/cones and 13 were hysterectomies including 5 HSIL and 35 SqCa. Infection caused by rare subtypes, hrHPV 45 and 52, were found in only two cases. Multi-subtype infection, detected in 28 % cases, was limited to HPV16/18 in all cases but one; one case showed hrHPV16/52 combination. CONCLUSION: A remarkable homogeneity of hrHPV 16 infection is noted in women with HSIL & SqCa in this country in these limited samples. This finding is in sharp contrast to the reports from western countries of frequent multi-viral and rare subtype hrHPV infection. This pilot study suggests that a vaccination program may be highly effective in controlling cervical cancer there. A larger study, however, is required to ratify the findings.
format Online
Article
Text
id pubmed-5034567
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-50345672016-09-29 Rare subtype(s) and frequency of multi-viral subtype high-risk human papilloma virus infection in high-grade squamous intraepithelial lesion and squamous cell carcinoma in a cervical carcinoma prevalent developing country: a multiplex real-time polymerase chain reaction-based pilot study Akhter, Shabnam Manna, Pradip Kamal, Mohammed Sung, C. James Lawrence, W. Dwayne Quddus, M. Ruhul Diagn Pathol Research BACKGROUND: Bangladesh, with a population of 160 million and nearly half being women, has the 4th highest rate of cervical carcinoma deaths in the world. It is projected that ∼500,000 of these women would die of this entirely preventable cancer by 2030. HPV vaccination is not widely offered in Bangladesh. This pilot study is designed to find out the prevalence of rare and multi-viral high-risk HPV (hrHPV) subtype(s) infection which may help strategize a large scale vaccination program in tackling cervical carcinoma in the country. METHODS: Forty cases of cervical High-Grade squamous intraepithelial lesion (HSIL) and Squamous cell carcinoma (SqCa) were collected. DNA was extracted from tissue representing HSIL and SqCa and multiplex PCR was run to identify all 15 hrHPV subtypes along with known positive controls. RESULTS: Of the total, 27 cases were biopsies/cones and 13 were hysterectomies including 5 HSIL and 35 SqCa. Infection caused by rare subtypes, hrHPV 45 and 52, were found in only two cases. Multi-subtype infection, detected in 28 % cases, was limited to HPV16/18 in all cases but one; one case showed hrHPV16/52 combination. CONCLUSION: A remarkable homogeneity of hrHPV 16 infection is noted in women with HSIL & SqCa in this country in these limited samples. This finding is in sharp contrast to the reports from western countries of frequent multi-viral and rare subtype hrHPV infection. This pilot study suggests that a vaccination program may be highly effective in controlling cervical cancer there. A larger study, however, is required to ratify the findings. BioMed Central 2016-09-22 /pmc/articles/PMC5034567/ /pubmed/27659129 http://dx.doi.org/10.1186/s13000-016-0542-y Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Akhter, Shabnam
Manna, Pradip
Kamal, Mohammed
Sung, C. James
Lawrence, W. Dwayne
Quddus, M. Ruhul
Rare subtype(s) and frequency of multi-viral subtype high-risk human papilloma virus infection in high-grade squamous intraepithelial lesion and squamous cell carcinoma in a cervical carcinoma prevalent developing country: a multiplex real-time polymerase chain reaction-based pilot study
title Rare subtype(s) and frequency of multi-viral subtype high-risk human papilloma virus infection in high-grade squamous intraepithelial lesion and squamous cell carcinoma in a cervical carcinoma prevalent developing country: a multiplex real-time polymerase chain reaction-based pilot study
title_full Rare subtype(s) and frequency of multi-viral subtype high-risk human papilloma virus infection in high-grade squamous intraepithelial lesion and squamous cell carcinoma in a cervical carcinoma prevalent developing country: a multiplex real-time polymerase chain reaction-based pilot study
title_fullStr Rare subtype(s) and frequency of multi-viral subtype high-risk human papilloma virus infection in high-grade squamous intraepithelial lesion and squamous cell carcinoma in a cervical carcinoma prevalent developing country: a multiplex real-time polymerase chain reaction-based pilot study
title_full_unstemmed Rare subtype(s) and frequency of multi-viral subtype high-risk human papilloma virus infection in high-grade squamous intraepithelial lesion and squamous cell carcinoma in a cervical carcinoma prevalent developing country: a multiplex real-time polymerase chain reaction-based pilot study
title_short Rare subtype(s) and frequency of multi-viral subtype high-risk human papilloma virus infection in high-grade squamous intraepithelial lesion and squamous cell carcinoma in a cervical carcinoma prevalent developing country: a multiplex real-time polymerase chain reaction-based pilot study
title_sort rare subtype(s) and frequency of multi-viral subtype high-risk human papilloma virus infection in high-grade squamous intraepithelial lesion and squamous cell carcinoma in a cervical carcinoma prevalent developing country: a multiplex real-time polymerase chain reaction-based pilot study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034567/
https://www.ncbi.nlm.nih.gov/pubmed/27659129
http://dx.doi.org/10.1186/s13000-016-0542-y
work_keys_str_mv AT akhtershabnam raresubtypesandfrequencyofmultiviralsubtypehighriskhumanpapillomavirusinfectioninhighgradesquamousintraepitheliallesionandsquamouscellcarcinomainacervicalcarcinomaprevalentdevelopingcountryamultiplexrealtimepolymerasechainreactionbasedpilotstudy
AT mannapradip raresubtypesandfrequencyofmultiviralsubtypehighriskhumanpapillomavirusinfectioninhighgradesquamousintraepitheliallesionandsquamouscellcarcinomainacervicalcarcinomaprevalentdevelopingcountryamultiplexrealtimepolymerasechainreactionbasedpilotstudy
AT kamalmohammed raresubtypesandfrequencyofmultiviralsubtypehighriskhumanpapillomavirusinfectioninhighgradesquamousintraepitheliallesionandsquamouscellcarcinomainacervicalcarcinomaprevalentdevelopingcountryamultiplexrealtimepolymerasechainreactionbasedpilotstudy
AT sungcjames raresubtypesandfrequencyofmultiviralsubtypehighriskhumanpapillomavirusinfectioninhighgradesquamousintraepitheliallesionandsquamouscellcarcinomainacervicalcarcinomaprevalentdevelopingcountryamultiplexrealtimepolymerasechainreactionbasedpilotstudy
AT lawrencewdwayne raresubtypesandfrequencyofmultiviralsubtypehighriskhumanpapillomavirusinfectioninhighgradesquamousintraepitheliallesionandsquamouscellcarcinomainacervicalcarcinomaprevalentdevelopingcountryamultiplexrealtimepolymerasechainreactionbasedpilotstudy
AT quddusmruhul raresubtypesandfrequencyofmultiviralsubtypehighriskhumanpapillomavirusinfectioninhighgradesquamousintraepitheliallesionandsquamouscellcarcinomainacervicalcarcinomaprevalentdevelopingcountryamultiplexrealtimepolymerasechainreactionbasedpilotstudy