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The inflammasome pathway in stable COPD and acute exacerbations
Chronic obstructive pulmonary disease (COPD) is characterised by pulmonary and systemic inflammation that bursts during exacerbations of the disease (ECOPD). The NLRP3 inflammasome is a key regulatory molecule of the inflammatory response. Its role in COPD is unclear. We investigated the NLRP3 infla...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Respiratory Society
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034597/ https://www.ncbi.nlm.nih.gov/pubmed/27730204 http://dx.doi.org/10.1183/23120541.00002-2016 |
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author | Faner, Rosa Sobradillo, Patricia Noguera, Aina Gomez, Cristina Cruz, Tamara López-Giraldo, Alejandra Ballester, Eugeni Soler, Nestor Arostegui, Juan I. Pelegrín, Pablo Rodriguez-Roisin, Roberto Yagüe, Jordi Cosio, Borja G. Juan, Manel Agustí, Alvar |
author_facet | Faner, Rosa Sobradillo, Patricia Noguera, Aina Gomez, Cristina Cruz, Tamara López-Giraldo, Alejandra Ballester, Eugeni Soler, Nestor Arostegui, Juan I. Pelegrín, Pablo Rodriguez-Roisin, Roberto Yagüe, Jordi Cosio, Borja G. Juan, Manel Agustí, Alvar |
author_sort | Faner, Rosa |
collection | PubMed |
description | Chronic obstructive pulmonary disease (COPD) is characterised by pulmonary and systemic inflammation that bursts during exacerbations of the disease (ECOPD). The NLRP3 inflammasome is a key regulatory molecule of the inflammatory response. Its role in COPD is unclear. We investigated the NLRP3 inflammasome status in: 1) lung tissue samples from 38 patients with stable COPD, 15 smokers with normal spirometry and 14 never-smokers; and 2) sputum and plasma samples from 56 ECOPD patients, of whom 41 could be reassessed at clinical recovery. We observed that: 1) in lung tissue samples of stable COPD patients, NLRP3 and interleukin (IL)-1β mRNA were upregulated, but both caspase-1 and ASC were mostly in inactive form, and 2) during infectious ECOPD, caspase-1, oligomeric ASC and associated cytokines (IL-1β, IL-18) were significantly increased in sputum compared with clinical recovery. The NLRP3 inflammasome is primed, but not activated, in the lungs of clinically stable COPD patients. Inflammasome activation occurs during infectious ECOPD. The results of this study suggest that the inflammasome participates in the inflammatory burst of infectious ECOPD. |
format | Online Article Text |
id | pubmed-5034597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | European Respiratory Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-50345972016-10-11 The inflammasome pathway in stable COPD and acute exacerbations Faner, Rosa Sobradillo, Patricia Noguera, Aina Gomez, Cristina Cruz, Tamara López-Giraldo, Alejandra Ballester, Eugeni Soler, Nestor Arostegui, Juan I. Pelegrín, Pablo Rodriguez-Roisin, Roberto Yagüe, Jordi Cosio, Borja G. Juan, Manel Agustí, Alvar ERJ Open Res Original Articles Chronic obstructive pulmonary disease (COPD) is characterised by pulmonary and systemic inflammation that bursts during exacerbations of the disease (ECOPD). The NLRP3 inflammasome is a key regulatory molecule of the inflammatory response. Its role in COPD is unclear. We investigated the NLRP3 inflammasome status in: 1) lung tissue samples from 38 patients with stable COPD, 15 smokers with normal spirometry and 14 never-smokers; and 2) sputum and plasma samples from 56 ECOPD patients, of whom 41 could be reassessed at clinical recovery. We observed that: 1) in lung tissue samples of stable COPD patients, NLRP3 and interleukin (IL)-1β mRNA were upregulated, but both caspase-1 and ASC were mostly in inactive form, and 2) during infectious ECOPD, caspase-1, oligomeric ASC and associated cytokines (IL-1β, IL-18) were significantly increased in sputum compared with clinical recovery. The NLRP3 inflammasome is primed, but not activated, in the lungs of clinically stable COPD patients. Inflammasome activation occurs during infectious ECOPD. The results of this study suggest that the inflammasome participates in the inflammatory burst of infectious ECOPD. European Respiratory Society 2016-07-11 /pmc/articles/PMC5034597/ /pubmed/27730204 http://dx.doi.org/10.1183/23120541.00002-2016 Text en The content of this work is ©the authors or their employers. Design and branding are ©ERS 2016 http://creativecommons.org/licenses/by-nc/4.0/ This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. |
spellingShingle | Original Articles Faner, Rosa Sobradillo, Patricia Noguera, Aina Gomez, Cristina Cruz, Tamara López-Giraldo, Alejandra Ballester, Eugeni Soler, Nestor Arostegui, Juan I. Pelegrín, Pablo Rodriguez-Roisin, Roberto Yagüe, Jordi Cosio, Borja G. Juan, Manel Agustí, Alvar The inflammasome pathway in stable COPD and acute exacerbations |
title | The inflammasome pathway in stable COPD and acute exacerbations |
title_full | The inflammasome pathway in stable COPD and acute exacerbations |
title_fullStr | The inflammasome pathway in stable COPD and acute exacerbations |
title_full_unstemmed | The inflammasome pathway in stable COPD and acute exacerbations |
title_short | The inflammasome pathway in stable COPD and acute exacerbations |
title_sort | inflammasome pathway in stable copd and acute exacerbations |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034597/ https://www.ncbi.nlm.nih.gov/pubmed/27730204 http://dx.doi.org/10.1183/23120541.00002-2016 |
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