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Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis
OBJECTIVE: DNA methylation may be a stable epigenetic contributor to defining fat cell lineage. METHODS: We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis. RE...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034609/ https://www.ncbi.nlm.nih.gov/pubmed/27689016 http://dx.doi.org/10.1016/j.molmet.2016.08.006 |
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author | Lim, Yen Ching Chia, Sook Yoong Jin, Shengnan Han, Weiping Ding, Chunming Sun, Lei |
author_facet | Lim, Yen Ching Chia, Sook Yoong Jin, Shengnan Han, Weiping Ding, Chunming Sun, Lei |
author_sort | Lim, Yen Ching |
collection | PubMed |
description | OBJECTIVE: DNA methylation may be a stable epigenetic contributor to defining fat cell lineage. METHODS: We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis. RESULTS: Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during, and after differentiation. We identified 31 genes whose promoters were significantly differentially methylated between white and brown adipogenesis at all three time points of differentiation. Among them, five genes belong to the Hox family; their expression levels were anti-correlated with promoter methylation, suggesting a regulatory role of DNA methylation in transcription. Blocking DNA methylation with 5-Aza-cytidine increased the expression of these genes, with the most prominent effect on Hoxc10, a repressor of BAT marker expression. CONCLUSIONS: Our data suggest that DNA methylation may play an important role in lineage-specific development in adipocytes. |
format | Online Article Text |
id | pubmed-5034609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-50346092016-09-29 Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis Lim, Yen Ching Chia, Sook Yoong Jin, Shengnan Han, Weiping Ding, Chunming Sun, Lei Mol Metab Brief Communication OBJECTIVE: DNA methylation may be a stable epigenetic contributor to defining fat cell lineage. METHODS: We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis. RESULTS: Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during, and after differentiation. We identified 31 genes whose promoters were significantly differentially methylated between white and brown adipogenesis at all three time points of differentiation. Among them, five genes belong to the Hox family; their expression levels were anti-correlated with promoter methylation, suggesting a regulatory role of DNA methylation in transcription. Blocking DNA methylation with 5-Aza-cytidine increased the expression of these genes, with the most prominent effect on Hoxc10, a repressor of BAT marker expression. CONCLUSIONS: Our data suggest that DNA methylation may play an important role in lineage-specific development in adipocytes. Elsevier 2016-08-17 /pmc/articles/PMC5034609/ /pubmed/27689016 http://dx.doi.org/10.1016/j.molmet.2016.08.006 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Communication Lim, Yen Ching Chia, Sook Yoong Jin, Shengnan Han, Weiping Ding, Chunming Sun, Lei Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_full | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_fullStr | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_full_unstemmed | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_short | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_sort | dynamic dna methylation landscape defines brown and white cell specificity during adipogenesis |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034609/ https://www.ncbi.nlm.nih.gov/pubmed/27689016 http://dx.doi.org/10.1016/j.molmet.2016.08.006 |
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