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Egg ovotransferrin‐derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats

SCOPE: Egg ovotransferrin‐derived angiotensin converting enzyme (ACE) inhibitory peptide IRW was previously shown to reduce blood pressure in spontaneously hypertensive rats through reduced vascular inflammation and increased nitric oxide‐mediated vasorelaxation. The main objective of the present st...

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Autores principales: Majumder, Kaustav, Liang, Guanxiang, Chen, Yanhong, Guan, LeLuo, Davidge, Sandra T., Wu, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034750/
https://www.ncbi.nlm.nih.gov/pubmed/26016560
http://dx.doi.org/10.1002/mnfr.201500050
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author Majumder, Kaustav
Liang, Guanxiang
Chen, Yanhong
Guan, LeLuo
Davidge, Sandra T.
Wu, Jianping
author_facet Majumder, Kaustav
Liang, Guanxiang
Chen, Yanhong
Guan, LeLuo
Davidge, Sandra T.
Wu, Jianping
author_sort Majumder, Kaustav
collection PubMed
description SCOPE: Egg ovotransferrin‐derived angiotensin converting enzyme (ACE) inhibitory peptide IRW was previously shown to reduce blood pressure in spontaneously hypertensive rats through reduced vascular inflammation and increased nitric oxide‐mediated vasorelaxation. The main objective of the present study was to investigate the molecular mechanism of this peptide through transcriptome analysis by RNAseq technique. METHODS AND RESULTS: Total RNA was extracted from kidney and mesenteric arteries; the RNAseq libraries (from untreated and IRW‐treated groups) were constructed and subjected to sequence using HiSeq 2000 system (Illumina) system. A total of 12 764 and 13 352 genes were detected in kidney and mesenteric arteries, respectively. The differentially expressed (DE) genes between untreated and IRW‐treated groups were identified and the functional analysis through ingenuity pathway analysis revealed a greater role of DE genes identified from mesenteric arteries than that of kidney in modulating various cardiovascular functions. Subsequent qPCR analysis further confirmed that IRW significantly increased the expression of ACE‐2, ABCB‐1, IRF‐8, and CDH‐1 while significantly decreased the expression ICAM‐1 and VCAM‐1 in mesenteric arteries. CONCLUSION: Our research showed for the first time that ACE inhibitory peptide IRW could contribute to its antihypertensive activity through increased ACE2 and decreased proinflammatory genes expression.
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spelling pubmed-50347502016-10-03 Egg ovotransferrin‐derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats Majumder, Kaustav Liang, Guanxiang Chen, Yanhong Guan, LeLuo Davidge, Sandra T. Wu, Jianping Mol Nutr Food Res Research Articles SCOPE: Egg ovotransferrin‐derived angiotensin converting enzyme (ACE) inhibitory peptide IRW was previously shown to reduce blood pressure in spontaneously hypertensive rats through reduced vascular inflammation and increased nitric oxide‐mediated vasorelaxation. The main objective of the present study was to investigate the molecular mechanism of this peptide through transcriptome analysis by RNAseq technique. METHODS AND RESULTS: Total RNA was extracted from kidney and mesenteric arteries; the RNAseq libraries (from untreated and IRW‐treated groups) were constructed and subjected to sequence using HiSeq 2000 system (Illumina) system. A total of 12 764 and 13 352 genes were detected in kidney and mesenteric arteries, respectively. The differentially expressed (DE) genes between untreated and IRW‐treated groups were identified and the functional analysis through ingenuity pathway analysis revealed a greater role of DE genes identified from mesenteric arteries than that of kidney in modulating various cardiovascular functions. Subsequent qPCR analysis further confirmed that IRW significantly increased the expression of ACE‐2, ABCB‐1, IRF‐8, and CDH‐1 while significantly decreased the expression ICAM‐1 and VCAM‐1 in mesenteric arteries. CONCLUSION: Our research showed for the first time that ACE inhibitory peptide IRW could contribute to its antihypertensive activity through increased ACE2 and decreased proinflammatory genes expression. John Wiley and Sons Inc. 2015-06-26 2015-09 /pmc/articles/PMC5034750/ /pubmed/26016560 http://dx.doi.org/10.1002/mnfr.201500050 Text en © 2015 The Authors. Molecular Nutrition & Food Research published by Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution‐Non‐Commercial‐NoDerivs Licence (http://creativecommons.org/licenses/by/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Majumder, Kaustav
Liang, Guanxiang
Chen, Yanhong
Guan, LeLuo
Davidge, Sandra T.
Wu, Jianping
Egg ovotransferrin‐derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats
title Egg ovotransferrin‐derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats
title_full Egg ovotransferrin‐derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats
title_fullStr Egg ovotransferrin‐derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats
title_full_unstemmed Egg ovotransferrin‐derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats
title_short Egg ovotransferrin‐derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats
title_sort egg ovotransferrin‐derived ace inhibitory peptide irw increases ace2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034750/
https://www.ncbi.nlm.nih.gov/pubmed/26016560
http://dx.doi.org/10.1002/mnfr.201500050
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