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KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella pneumoniae

Health care-acquired infections (HAIs) kill tens of thousands of people each year and add significantly to health care costs. Multidrug-resistant and epidemic strains are a large proportion of HAI agents, and multidrug-resistant strains of Klebsiella pneumoniae, a leading HAI agent, have caused an u...

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Autores principales: Bowers, Jolene R., Lemmer, Darrin, Sahl, Jason W., Pearson, Talima, Driebe, Elizabeth M., Wojack, Bette, Saubolle, Michael A., Engelthaler, David M., Keim, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035412/
https://www.ncbi.nlm.nih.gov/pubmed/27510832
http://dx.doi.org/10.1128/JCM.00927-16
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author Bowers, Jolene R.
Lemmer, Darrin
Sahl, Jason W.
Pearson, Talima
Driebe, Elizabeth M.
Wojack, Bette
Saubolle, Michael A.
Engelthaler, David M.
Keim, Paul
author_facet Bowers, Jolene R.
Lemmer, Darrin
Sahl, Jason W.
Pearson, Talima
Driebe, Elizabeth M.
Wojack, Bette
Saubolle, Michael A.
Engelthaler, David M.
Keim, Paul
author_sort Bowers, Jolene R.
collection PubMed
description Health care-acquired infections (HAIs) kill tens of thousands of people each year and add significantly to health care costs. Multidrug-resistant and epidemic strains are a large proportion of HAI agents, and multidrug-resistant strains of Klebsiella pneumoniae, a leading HAI agent, have caused an urgent public health crisis. In the health care environment, patient colonization by K. pneumoniae precedes infection, and transmission via colonization leads to outbreaks. Periodic patient screening for K. pneumoniae colonization has the potential to curb the number of HAIs. In this report, we describe the design and validation of KlebSeq, a highly informative screening tool that detects Klebsiella species and identifies clinically important strains and characteristics by using highly multiplexed amplicon sequencing without a live-culturing step. We demonstrate the utility of this tool on several complex specimen types, including urine, wound swabs and tissue, and several types of respiratory and fecal specimens, showing K. pneumoniae species and clonal group identification and antimicrobial resistance and virulence profiling, including capsule typing. Use of this amplicon sequencing tool to screen patients for Klebsiella carriage could inform health care staff of the risk of infection and outbreak potential. KlebSeq also serves as a model for next-generation molecular tools for public health and health care, as expansion of this tool can be used for several other HAI agents or applications.
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spelling pubmed-50354122016-10-04 KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella pneumoniae Bowers, Jolene R. Lemmer, Darrin Sahl, Jason W. Pearson, Talima Driebe, Elizabeth M. Wojack, Bette Saubolle, Michael A. Engelthaler, David M. Keim, Paul J Clin Microbiol Bacteriology Health care-acquired infections (HAIs) kill tens of thousands of people each year and add significantly to health care costs. Multidrug-resistant and epidemic strains are a large proportion of HAI agents, and multidrug-resistant strains of Klebsiella pneumoniae, a leading HAI agent, have caused an urgent public health crisis. In the health care environment, patient colonization by K. pneumoniae precedes infection, and transmission via colonization leads to outbreaks. Periodic patient screening for K. pneumoniae colonization has the potential to curb the number of HAIs. In this report, we describe the design and validation of KlebSeq, a highly informative screening tool that detects Klebsiella species and identifies clinically important strains and characteristics by using highly multiplexed amplicon sequencing without a live-culturing step. We demonstrate the utility of this tool on several complex specimen types, including urine, wound swabs and tissue, and several types of respiratory and fecal specimens, showing K. pneumoniae species and clonal group identification and antimicrobial resistance and virulence profiling, including capsule typing. Use of this amplicon sequencing tool to screen patients for Klebsiella carriage could inform health care staff of the risk of infection and outbreak potential. KlebSeq also serves as a model for next-generation molecular tools for public health and health care, as expansion of this tool can be used for several other HAI agents or applications. American Society for Microbiology 2016-09-23 2016-10 /pmc/articles/PMC5035412/ /pubmed/27510832 http://dx.doi.org/10.1128/JCM.00927-16 Text en Copyright © 2016 Bowers et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Bacteriology
Bowers, Jolene R.
Lemmer, Darrin
Sahl, Jason W.
Pearson, Talima
Driebe, Elizabeth M.
Wojack, Bette
Saubolle, Michael A.
Engelthaler, David M.
Keim, Paul
KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella pneumoniae
title KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella pneumoniae
title_full KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella pneumoniae
title_fullStr KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella pneumoniae
title_full_unstemmed KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella pneumoniae
title_short KlebSeq, a Diagnostic Tool for Surveillance, Detection, and Monitoring of Klebsiella pneumoniae
title_sort klebseq, a diagnostic tool for surveillance, detection, and monitoring of klebsiella pneumoniae
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035412/
https://www.ncbi.nlm.nih.gov/pubmed/27510832
http://dx.doi.org/10.1128/JCM.00927-16
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