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A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice
Cardiac regeneration is one of the prime visions in cardiovascular research. The mouse neonatal apical resection and left anterior descending artery (LAD) ligation model introduced novel in vivo mammalian assays to study cardiac regeneration. However, recent reports and editorials discussed and crit...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035663/ https://www.ncbi.nlm.nih.gov/pubmed/27665606 http://dx.doi.org/10.1007/s00395-016-0580-3 |
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author | Haubner, Bernhard J. Schuetz, Thomas Penninger, Josef M. |
author_facet | Haubner, Bernhard J. Schuetz, Thomas Penninger, Josef M. |
author_sort | Haubner, Bernhard J. |
collection | PubMed |
description | Cardiac regeneration is one of the prime visions in cardiovascular research. The mouse neonatal apical resection and left anterior descending artery (LAD) ligation model introduced novel in vivo mammalian assays to study cardiac regeneration. However, recent reports and editorials discussed and critically questioned the value and technical reproducibility of the mouse neonatal myocardial infarction approach, making it paramount to develop and use a reproducible model system. We established a mouse neonatal myocardial infarction model by visually confirmed ligation of the LAD using microsurgery. TdT-mediated dUTP nick-end labeling (TUNEL) proved reproducible massive myocardial infarctions in a defined region of the apex and anterior wall of neonatal and 7-day-old mice. Whereas hearts ligated on postnatal day 7 displayed chronic injury, cardiac samples ligated immediately after birth always showed complete structural regeneration after long-term follow-up. Cardiac regeneration was observed in all mouse stains (C57BL/6J, ICR, and mixed background C57BL/6JxSv129) tested so far. We present a detailed in vivo protocol to study complex mechanisms of complete cardiac repair following ischemic cardiac damage. Neonatal LAD ligation surgery is feasible, and results in reproducible myocardial infarctions 24 h after ligation, and no structural myocardial defects are detectable following long-term follow-up. We encourage the cardiovascular community to use our protocol and teaching video to answer key scientific questions in the field of cardiac regeneration. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00395-016-0580-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5035663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-50356632016-10-09 A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice Haubner, Bernhard J. Schuetz, Thomas Penninger, Josef M. Basic Res Cardiol Original Contribution Cardiac regeneration is one of the prime visions in cardiovascular research. The mouse neonatal apical resection and left anterior descending artery (LAD) ligation model introduced novel in vivo mammalian assays to study cardiac regeneration. However, recent reports and editorials discussed and critically questioned the value and technical reproducibility of the mouse neonatal myocardial infarction approach, making it paramount to develop and use a reproducible model system. We established a mouse neonatal myocardial infarction model by visually confirmed ligation of the LAD using microsurgery. TdT-mediated dUTP nick-end labeling (TUNEL) proved reproducible massive myocardial infarctions in a defined region of the apex and anterior wall of neonatal and 7-day-old mice. Whereas hearts ligated on postnatal day 7 displayed chronic injury, cardiac samples ligated immediately after birth always showed complete structural regeneration after long-term follow-up. Cardiac regeneration was observed in all mouse stains (C57BL/6J, ICR, and mixed background C57BL/6JxSv129) tested so far. We present a detailed in vivo protocol to study complex mechanisms of complete cardiac repair following ischemic cardiac damage. Neonatal LAD ligation surgery is feasible, and results in reproducible myocardial infarctions 24 h after ligation, and no structural myocardial defects are detectable following long-term follow-up. We encourage the cardiovascular community to use our protocol and teaching video to answer key scientific questions in the field of cardiac regeneration. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00395-016-0580-3) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-09-24 2016 /pmc/articles/PMC5035663/ /pubmed/27665606 http://dx.doi.org/10.1007/s00395-016-0580-3 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Contribution Haubner, Bernhard J. Schuetz, Thomas Penninger, Josef M. A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice |
title | A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice |
title_full | A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice |
title_fullStr | A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice |
title_full_unstemmed | A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice |
title_short | A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice |
title_sort | reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice |
topic | Original Contribution |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035663/ https://www.ncbi.nlm.nih.gov/pubmed/27665606 http://dx.doi.org/10.1007/s00395-016-0580-3 |
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