Cargando…
Resource Partitioning between Bacteria, Fungi, and Protists in the Detritusphere of an Agricultural Soil
The flow of plant-derived carbon in soil is a key component of global carbon cycling. Conceptual models of trophic carbon fluxes in soil have assumed separate bacterial and fungal energy channels in the detritusphere, controlled by both substrate complexity and recalcitrance. However, detailed under...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035733/ https://www.ncbi.nlm.nih.gov/pubmed/27725815 http://dx.doi.org/10.3389/fmicb.2016.01524 |
_version_ | 1782455442847301632 |
---|---|
author | Kramer, Susanne Dibbern, Dörte Moll, Julia Huenninghaus, Maike Koller, Robert Krueger, Dirk Marhan, Sven Urich, Tim Wubet, Tesfaye Bonkowski, Michael Buscot, François Lueders, Tillmann Kandeler, Ellen |
author_facet | Kramer, Susanne Dibbern, Dörte Moll, Julia Huenninghaus, Maike Koller, Robert Krueger, Dirk Marhan, Sven Urich, Tim Wubet, Tesfaye Bonkowski, Michael Buscot, François Lueders, Tillmann Kandeler, Ellen |
author_sort | Kramer, Susanne |
collection | PubMed |
description | The flow of plant-derived carbon in soil is a key component of global carbon cycling. Conceptual models of trophic carbon fluxes in soil have assumed separate bacterial and fungal energy channels in the detritusphere, controlled by both substrate complexity and recalcitrance. However, detailed understanding of the key populations involved and niche-partitioning between them is limited. Here, a microcosm experiment was performed to trace the flow of detritusphere C from substrate analogs (glucose, cellulose) and plant biomass amendments (maize leaves, roots) in an agricultural soil. Carbon flow was traced by rRNA stable isotope probing and amplicon sequencing across three microbial kingdoms. Distinct lineages within the Actinobacteria, Bacteroidetes, Gammaproteobacteria, Basidiomycota, Ascomycota as well as Peronosporomycetes were identified as important primary substrate consumers. A dynamic succession of primary consumers was observed especially in the cellulose treatments, but also in plant amendments over time. While intra-kingdom niche partitioning was clearly observed, distinct bacterial and fungal energy channels were not apparent. Furthermore, while the diversity of primary substrate consumers did not notably increase with substrate complexity, consumer succession and secondary trophic links to bacterivorous and fungivorous microbes resulted in increased food web complexity in the more recalcitrant substrates. This suggests that rather than substrate-defined energy channels, consumer succession as well as intra- and inter-kingdom cross-feeding should be considered as mechanisms supporting food web complexity in the detritusphere. |
format | Online Article Text |
id | pubmed-5035733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50357332016-10-10 Resource Partitioning between Bacteria, Fungi, and Protists in the Detritusphere of an Agricultural Soil Kramer, Susanne Dibbern, Dörte Moll, Julia Huenninghaus, Maike Koller, Robert Krueger, Dirk Marhan, Sven Urich, Tim Wubet, Tesfaye Bonkowski, Michael Buscot, François Lueders, Tillmann Kandeler, Ellen Front Microbiol Microbiology The flow of plant-derived carbon in soil is a key component of global carbon cycling. Conceptual models of trophic carbon fluxes in soil have assumed separate bacterial and fungal energy channels in the detritusphere, controlled by both substrate complexity and recalcitrance. However, detailed understanding of the key populations involved and niche-partitioning between them is limited. Here, a microcosm experiment was performed to trace the flow of detritusphere C from substrate analogs (glucose, cellulose) and plant biomass amendments (maize leaves, roots) in an agricultural soil. Carbon flow was traced by rRNA stable isotope probing and amplicon sequencing across three microbial kingdoms. Distinct lineages within the Actinobacteria, Bacteroidetes, Gammaproteobacteria, Basidiomycota, Ascomycota as well as Peronosporomycetes were identified as important primary substrate consumers. A dynamic succession of primary consumers was observed especially in the cellulose treatments, but also in plant amendments over time. While intra-kingdom niche partitioning was clearly observed, distinct bacterial and fungal energy channels were not apparent. Furthermore, while the diversity of primary substrate consumers did not notably increase with substrate complexity, consumer succession and secondary trophic links to bacterivorous and fungivorous microbes resulted in increased food web complexity in the more recalcitrant substrates. This suggests that rather than substrate-defined energy channels, consumer succession as well as intra- and inter-kingdom cross-feeding should be considered as mechanisms supporting food web complexity in the detritusphere. Frontiers Media S.A. 2016-09-26 /pmc/articles/PMC5035733/ /pubmed/27725815 http://dx.doi.org/10.3389/fmicb.2016.01524 Text en Copyright © 2016 Kramer, Dibbern, Moll, Huenninghaus, Koller, Krueger, Marhan, Urich, Wubet, Bonkowski, Buscot, Lueders and Kandeler. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Kramer, Susanne Dibbern, Dörte Moll, Julia Huenninghaus, Maike Koller, Robert Krueger, Dirk Marhan, Sven Urich, Tim Wubet, Tesfaye Bonkowski, Michael Buscot, François Lueders, Tillmann Kandeler, Ellen Resource Partitioning between Bacteria, Fungi, and Protists in the Detritusphere of an Agricultural Soil |
title | Resource Partitioning between Bacteria, Fungi, and Protists in the Detritusphere of an Agricultural Soil |
title_full | Resource Partitioning between Bacteria, Fungi, and Protists in the Detritusphere of an Agricultural Soil |
title_fullStr | Resource Partitioning between Bacteria, Fungi, and Protists in the Detritusphere of an Agricultural Soil |
title_full_unstemmed | Resource Partitioning between Bacteria, Fungi, and Protists in the Detritusphere of an Agricultural Soil |
title_short | Resource Partitioning between Bacteria, Fungi, and Protists in the Detritusphere of an Agricultural Soil |
title_sort | resource partitioning between bacteria, fungi, and protists in the detritusphere of an agricultural soil |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035733/ https://www.ncbi.nlm.nih.gov/pubmed/27725815 http://dx.doi.org/10.3389/fmicb.2016.01524 |
work_keys_str_mv | AT kramersusanne resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT dibberndorte resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT molljulia resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT huenninghausmaike resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT kollerrobert resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT kruegerdirk resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT marhansven resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT urichtim resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT wubettesfaye resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT bonkowskimichael resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT buscotfrancois resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT luederstillmann resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT kandelerellen resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil |