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Cell Culture Models for the Investigation of Hepatitis B and D Virus Infection

Chronic hepatitis B virus (HBV) and hepatitis D virus (HDV) infections are major causes of liver disease and hepatocellular carcinoma worldwide. Despite the presence of an efficient preventive vaccine, more than 250 million patients are chronically infected with HBV. Current antivirals effectively c...

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Autores principales: Verrier, Eloi R., Colpitts, Che C., Schuster, Catherine, Zeisel, Mirjam B., Baumert, Thomas F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035974/
https://www.ncbi.nlm.nih.gov/pubmed/27657111
http://dx.doi.org/10.3390/v8090261
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author Verrier, Eloi R.
Colpitts, Che C.
Schuster, Catherine
Zeisel, Mirjam B.
Baumert, Thomas F.
author_facet Verrier, Eloi R.
Colpitts, Che C.
Schuster, Catherine
Zeisel, Mirjam B.
Baumert, Thomas F.
author_sort Verrier, Eloi R.
collection PubMed
description Chronic hepatitis B virus (HBV) and hepatitis D virus (HDV) infections are major causes of liver disease and hepatocellular carcinoma worldwide. Despite the presence of an efficient preventive vaccine, more than 250 million patients are chronically infected with HBV. Current antivirals effectively control but only rarely cure chronic infection. While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. Native hepatoma cell lines do not allow viral infection, and the culture of primary hepatocytes, the natural host cell for the viruses, implies a series of constraints restricting the possibilities of analyzing virus-host interactions. Recently, the discovery of the sodium taurocholate co-transporting polypeptide (NTCP) as a key HBV/HDV cell entry factor has opened the door to a new era of investigation, as NTCP-overexpressing hepatoma cells acquire susceptibility to HBV and HDV infections. In this review, we summarize the major cell culture models for HBV and HDV infection, discuss their advantages and limitations and highlight perspectives for future developments.
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spelling pubmed-50359742016-09-29 Cell Culture Models for the Investigation of Hepatitis B and D Virus Infection Verrier, Eloi R. Colpitts, Che C. Schuster, Catherine Zeisel, Mirjam B. Baumert, Thomas F. Viruses Review Chronic hepatitis B virus (HBV) and hepatitis D virus (HDV) infections are major causes of liver disease and hepatocellular carcinoma worldwide. Despite the presence of an efficient preventive vaccine, more than 250 million patients are chronically infected with HBV. Current antivirals effectively control but only rarely cure chronic infection. While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. Native hepatoma cell lines do not allow viral infection, and the culture of primary hepatocytes, the natural host cell for the viruses, implies a series of constraints restricting the possibilities of analyzing virus-host interactions. Recently, the discovery of the sodium taurocholate co-transporting polypeptide (NTCP) as a key HBV/HDV cell entry factor has opened the door to a new era of investigation, as NTCP-overexpressing hepatoma cells acquire susceptibility to HBV and HDV infections. In this review, we summarize the major cell culture models for HBV and HDV infection, discuss their advantages and limitations and highlight perspectives for future developments. MDPI 2016-09-20 /pmc/articles/PMC5035974/ /pubmed/27657111 http://dx.doi.org/10.3390/v8090261 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Verrier, Eloi R.
Colpitts, Che C.
Schuster, Catherine
Zeisel, Mirjam B.
Baumert, Thomas F.
Cell Culture Models for the Investigation of Hepatitis B and D Virus Infection
title Cell Culture Models for the Investigation of Hepatitis B and D Virus Infection
title_full Cell Culture Models for the Investigation of Hepatitis B and D Virus Infection
title_fullStr Cell Culture Models for the Investigation of Hepatitis B and D Virus Infection
title_full_unstemmed Cell Culture Models for the Investigation of Hepatitis B and D Virus Infection
title_short Cell Culture Models for the Investigation of Hepatitis B and D Virus Infection
title_sort cell culture models for the investigation of hepatitis b and d virus infection
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5035974/
https://www.ncbi.nlm.nih.gov/pubmed/27657111
http://dx.doi.org/10.3390/v8090261
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