Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery

In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expr...

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Autores principales: Shi, Jian, Li, Yuan, Liang, Shuzhen, Zeng, Jianying, Liu, Guifeng, Mu, Feng, Li, Haibo, Chen, Jibing, Liu, Tongjun, Niu, Lizhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036405/
https://www.ncbi.nlm.nih.gov/pubmed/27415969
http://dx.doi.org/10.1080/15384047.2016.1210731
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author Shi, Jian
Li, Yuan
Liang, Shuzhen
Zeng, Jianying
Liu, Guifeng
Mu, Feng
Li, Haibo
Chen, Jibing
Liu, Tongjun
Niu, Lizhi
author_facet Shi, Jian
Li, Yuan
Liang, Shuzhen
Zeng, Jianying
Liu, Guifeng
Mu, Feng
Li, Haibo
Chen, Jibing
Liu, Tongjun
Niu, Lizhi
author_sort Shi, Jian
collection PubMed
description In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.
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spelling pubmed-50364052016-10-05 Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery Shi, Jian Li, Yuan Liang, Shuzhen Zeng, Jianying Liu, Guifeng Mu, Feng Li, Haibo Chen, Jibing Liu, Tongjun Niu, Lizhi Cancer Biol Ther Research Paper In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer. Taylor & Francis 2016-07-14 /pmc/articles/PMC5036405/ /pubmed/27415969 http://dx.doi.org/10.1080/15384047.2016.1210731 Text en © 2016 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Research Paper
Shi, Jian
Li, Yuan
Liang, Shuzhen
Zeng, Jianying
Liu, Guifeng
Mu, Feng
Li, Haibo
Chen, Jibing
Liu, Tongjun
Niu, Lizhi
Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
title Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
title_full Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
title_fullStr Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
title_full_unstemmed Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
title_short Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
title_sort analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036405/
https://www.ncbi.nlm.nih.gov/pubmed/27415969
http://dx.doi.org/10.1080/15384047.2016.1210731
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