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The impact of aminopyrene trisulfonate (APTS) label in acceptor glycan substrates for profiling plant pectin β-galactosyltransferase activities
Aminopyrene trisulfonate (APTS)-labelled disaccharides are demonstrated to serve as readily accessible acceptor substrates for galactosyltransferase activities present in Arabidopsis microsome preparations. The reductive amination procedure used to install the fluorophore results in loss of the ring...
Autores principales: | Goetz, Stephan, Rejzek, Martin, Nepogodiev, Sergey A., Field, Robert A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036537/ https://www.ncbi.nlm.nih.gov/pubmed/27479753 http://dx.doi.org/10.1016/j.carres.2016.07.017 |
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