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The pressure-temperature phase diagram of hen lysozyme at low pH
The equilibrium unfolding of hen lysozyme at pH 2 was studied as a function of pressure (0.1~700MPa) and temperature (−10°C~50°C) using Trp fluorescence as monitor supplemented by variable pressure (1)H NMR spectroscopy (0.1~400MPa). The unfolding profiles monitored by the two methods allowed the tw...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Biophysical Society of Japan (BSJ)
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036640/ https://www.ncbi.nlm.nih.gov/pubmed/27857574 http://dx.doi.org/10.2142/biophysics.5.1 |