Cargando…
Estimation for diameter of superparamagnetic particles in Daphnia resting eggs
Ferromagnetic resonance (FMR) with an electron spin resonance (ESR) apparatus was investigated for super-paramagnetic particles within Daphnia resting eggs. High-field (HF) resonance lines near g=2 resulted from single superparamagnetic particles, were detected from ESR spectra of Daphnia resting eg...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society of Japan (BSJ)
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036663/ https://www.ncbi.nlm.nih.gov/pubmed/27857585 http://dx.doi.org/10.2142/biophysics.6.53 |
_version_ | 1782455594694737920 |
---|---|
author | Sakata, Masanobu Kawasaki, Tamami Shibue, Toshimichi Namiki, Hideo |
author_facet | Sakata, Masanobu Kawasaki, Tamami Shibue, Toshimichi Namiki, Hideo |
author_sort | Sakata, Masanobu |
collection | PubMed |
description | Ferromagnetic resonance (FMR) with an electron spin resonance (ESR) apparatus was investigated for super-paramagnetic particles within Daphnia resting eggs. High-field (HF) resonance lines near g=2 resulted from single superparamagnetic particles, were detected from ESR spectra of Daphnia resting eggs. The size of isolated superparamagnetic particles within Daphnia resting eggs was calculated to be approximately 13 nm in diameter by analysis of the temperature dependence of the HF line width. Small-angle X-ray scattering (SAXS) analysis of Daphnia resting eggs also showed that average size of superparamagnetic particles in diameter, equivalent to magnetite, was approximately 13 nm. The combination of FMR and SAXS measurement is very effective in estimating the size of superparamagnetic particles in biological organisms, with difficulties of preparing for samples for measurement by electron microscopy. However, Chlorella, with that Daphnia were raised, did not show FMR spectra, showing no magnetic particles within Daphnia resting eggs. Therefore, it suggested that superparamagnetic particles within Daphnia resting eggs, were mineralized in Daphnia as the result of biomineralization of Fe originated from Chlorella. |
format | Online Article Text |
id | pubmed-5036663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Biophysical Society of Japan (BSJ) |
record_format | MEDLINE/PubMed |
spelling | pubmed-50366632016-11-17 Estimation for diameter of superparamagnetic particles in Daphnia resting eggs Sakata, Masanobu Kawasaki, Tamami Shibue, Toshimichi Namiki, Hideo Biophysics (Nagoya-shi) Articles Ferromagnetic resonance (FMR) with an electron spin resonance (ESR) apparatus was investigated for super-paramagnetic particles within Daphnia resting eggs. High-field (HF) resonance lines near g=2 resulted from single superparamagnetic particles, were detected from ESR spectra of Daphnia resting eggs. The size of isolated superparamagnetic particles within Daphnia resting eggs was calculated to be approximately 13 nm in diameter by analysis of the temperature dependence of the HF line width. Small-angle X-ray scattering (SAXS) analysis of Daphnia resting eggs also showed that average size of superparamagnetic particles in diameter, equivalent to magnetite, was approximately 13 nm. The combination of FMR and SAXS measurement is very effective in estimating the size of superparamagnetic particles in biological organisms, with difficulties of preparing for samples for measurement by electron microscopy. However, Chlorella, with that Daphnia were raised, did not show FMR spectra, showing no magnetic particles within Daphnia resting eggs. Therefore, it suggested that superparamagnetic particles within Daphnia resting eggs, were mineralized in Daphnia as the result of biomineralization of Fe originated from Chlorella. The Biophysical Society of Japan (BSJ) 2010-12-07 /pmc/articles/PMC5036663/ /pubmed/27857585 http://dx.doi.org/10.2142/biophysics.6.53 Text en 2010 © The Biophysical Society of Japan |
spellingShingle | Articles Sakata, Masanobu Kawasaki, Tamami Shibue, Toshimichi Namiki, Hideo Estimation for diameter of superparamagnetic particles in Daphnia resting eggs |
title | Estimation for diameter of superparamagnetic particles in Daphnia resting eggs |
title_full | Estimation for diameter of superparamagnetic particles in Daphnia resting eggs |
title_fullStr | Estimation for diameter of superparamagnetic particles in Daphnia resting eggs |
title_full_unstemmed | Estimation for diameter of superparamagnetic particles in Daphnia resting eggs |
title_short | Estimation for diameter of superparamagnetic particles in Daphnia resting eggs |
title_sort | estimation for diameter of superparamagnetic particles in daphnia resting eggs |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036663/ https://www.ncbi.nlm.nih.gov/pubmed/27857585 http://dx.doi.org/10.2142/biophysics.6.53 |
work_keys_str_mv | AT sakatamasanobu estimationfordiameterofsuperparamagneticparticlesindaphniarestingeggs AT kawasakitamami estimationfordiameterofsuperparamagneticparticlesindaphniarestingeggs AT shibuetoshimichi estimationfordiameterofsuperparamagneticparticlesindaphniarestingeggs AT namikihideo estimationfordiameterofsuperparamagneticparticlesindaphniarestingeggs |