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Gamma-tubulin coordinates nuclear envelope assembly around chromatin

The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and...

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Autores principales: Rosselló, Catalina Ana, Lindström, Lisa, Glindre, Johan, Eklund, Greta, Alvarado-Kristensson, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037270/
https://www.ncbi.nlm.nih.gov/pubmed/27699285
http://dx.doi.org/10.1016/j.heliyon.2016.e00166
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author Rosselló, Catalina Ana
Lindström, Lisa
Glindre, Johan
Eklund, Greta
Alvarado-Kristensson, Maria
author_facet Rosselló, Catalina Ana
Lindström, Lisa
Glindre, Johan
Eklund, Greta
Alvarado-Kristensson, Maria
author_sort Rosselló, Catalina Ana
collection PubMed
description The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a constant interplay between the chromatin-associated and the cytosolic pools of γ-tubulin is required and, when the balance between pools is impaired, aberrant nuclei are formed. We therefore propose that the nuclear protein meshwork formed by γ-tubulin around chromatin coordinates nuclear formation in eukaryotic cells.
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spelling pubmed-50372702016-10-03 Gamma-tubulin coordinates nuclear envelope assembly around chromatin Rosselló, Catalina Ana Lindström, Lisa Glindre, Johan Eklund, Greta Alvarado-Kristensson, Maria Heliyon Article The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a constant interplay between the chromatin-associated and the cytosolic pools of γ-tubulin is required and, when the balance between pools is impaired, aberrant nuclei are formed. We therefore propose that the nuclear protein meshwork formed by γ-tubulin around chromatin coordinates nuclear formation in eukaryotic cells. Elsevier 2016-09-24 /pmc/articles/PMC5037270/ /pubmed/27699285 http://dx.doi.org/10.1016/j.heliyon.2016.e00166 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Rosselló, Catalina Ana
Lindström, Lisa
Glindre, Johan
Eklund, Greta
Alvarado-Kristensson, Maria
Gamma-tubulin coordinates nuclear envelope assembly around chromatin
title Gamma-tubulin coordinates nuclear envelope assembly around chromatin
title_full Gamma-tubulin coordinates nuclear envelope assembly around chromatin
title_fullStr Gamma-tubulin coordinates nuclear envelope assembly around chromatin
title_full_unstemmed Gamma-tubulin coordinates nuclear envelope assembly around chromatin
title_short Gamma-tubulin coordinates nuclear envelope assembly around chromatin
title_sort gamma-tubulin coordinates nuclear envelope assembly around chromatin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037270/
https://www.ncbi.nlm.nih.gov/pubmed/27699285
http://dx.doi.org/10.1016/j.heliyon.2016.e00166
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