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Dissociation and Re-Aggregation of Multicell-Ensheathed Fragments Responsible for Rapid Production of Massive Clumps of Leptothrix Sheaths

Species of the Fe/Mn-oxidizing bacteria Leptothrix produce tremendous amounts of microtubular, Fe/Mn-encrusted sheaths within a few days in outwells of groundwater that can rapidly clog water systems. To understand this mode of rapid sheath production and define the timescales involved, behaviors of...

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Autores principales: Kunoh, Tatsuki, Nagaoka, Noriyuki, McFarlane, Ian R., Tamura, Katsunori, El-Naggar, Mohamed Y., Kunoh, Hitoshi, Takada, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037351/
https://www.ncbi.nlm.nih.gov/pubmed/27490579
http://dx.doi.org/10.3390/biology5030032
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author Kunoh, Tatsuki
Nagaoka, Noriyuki
McFarlane, Ian R.
Tamura, Katsunori
El-Naggar, Mohamed Y.
Kunoh, Hitoshi
Takada, Jun
author_facet Kunoh, Tatsuki
Nagaoka, Noriyuki
McFarlane, Ian R.
Tamura, Katsunori
El-Naggar, Mohamed Y.
Kunoh, Hitoshi
Takada, Jun
author_sort Kunoh, Tatsuki
collection PubMed
description Species of the Fe/Mn-oxidizing bacteria Leptothrix produce tremendous amounts of microtubular, Fe/Mn-encrusted sheaths within a few days in outwells of groundwater that can rapidly clog water systems. To understand this mode of rapid sheath production and define the timescales involved, behaviors of sheath-forming Leptothrix sp. strain OUMS1 were examined using time-lapse video at the initial stage of sheath formation. OUMS1 formed clumps of tangled sheaths. Electron microscopy confirmed the presence of a thin layer of bacterial exopolymer fibrils around catenulate cells (corresponding to the immature sheath). In time-lapse videos, numerous sheath filaments that extended from the periphery of sheath clumps repeatedly fragmented at the apex of the same fragment, the fragments then aggregated and again elongated, eventually forming a large sheath clump comprising tangled sheaths within two days. In this study, we found that fast microscopic fragmentation, dissociation, re-aggregation and re-elongation events are the basis of the rapid, massive production of Leptothrix sheaths typically observed at macroscopic scales.
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spelling pubmed-50373512016-09-29 Dissociation and Re-Aggregation of Multicell-Ensheathed Fragments Responsible for Rapid Production of Massive Clumps of Leptothrix Sheaths Kunoh, Tatsuki Nagaoka, Noriyuki McFarlane, Ian R. Tamura, Katsunori El-Naggar, Mohamed Y. Kunoh, Hitoshi Takada, Jun Biology (Basel) Article Species of the Fe/Mn-oxidizing bacteria Leptothrix produce tremendous amounts of microtubular, Fe/Mn-encrusted sheaths within a few days in outwells of groundwater that can rapidly clog water systems. To understand this mode of rapid sheath production and define the timescales involved, behaviors of sheath-forming Leptothrix sp. strain OUMS1 were examined using time-lapse video at the initial stage of sheath formation. OUMS1 formed clumps of tangled sheaths. Electron microscopy confirmed the presence of a thin layer of bacterial exopolymer fibrils around catenulate cells (corresponding to the immature sheath). In time-lapse videos, numerous sheath filaments that extended from the periphery of sheath clumps repeatedly fragmented at the apex of the same fragment, the fragments then aggregated and again elongated, eventually forming a large sheath clump comprising tangled sheaths within two days. In this study, we found that fast microscopic fragmentation, dissociation, re-aggregation and re-elongation events are the basis of the rapid, massive production of Leptothrix sheaths typically observed at macroscopic scales. MDPI 2016-08-01 /pmc/articles/PMC5037351/ /pubmed/27490579 http://dx.doi.org/10.3390/biology5030032 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kunoh, Tatsuki
Nagaoka, Noriyuki
McFarlane, Ian R.
Tamura, Katsunori
El-Naggar, Mohamed Y.
Kunoh, Hitoshi
Takada, Jun
Dissociation and Re-Aggregation of Multicell-Ensheathed Fragments Responsible for Rapid Production of Massive Clumps of Leptothrix Sheaths
title Dissociation and Re-Aggregation of Multicell-Ensheathed Fragments Responsible for Rapid Production of Massive Clumps of Leptothrix Sheaths
title_full Dissociation and Re-Aggregation of Multicell-Ensheathed Fragments Responsible for Rapid Production of Massive Clumps of Leptothrix Sheaths
title_fullStr Dissociation and Re-Aggregation of Multicell-Ensheathed Fragments Responsible for Rapid Production of Massive Clumps of Leptothrix Sheaths
title_full_unstemmed Dissociation and Re-Aggregation of Multicell-Ensheathed Fragments Responsible for Rapid Production of Massive Clumps of Leptothrix Sheaths
title_short Dissociation and Re-Aggregation of Multicell-Ensheathed Fragments Responsible for Rapid Production of Massive Clumps of Leptothrix Sheaths
title_sort dissociation and re-aggregation of multicell-ensheathed fragments responsible for rapid production of massive clumps of leptothrix sheaths
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037351/
https://www.ncbi.nlm.nih.gov/pubmed/27490579
http://dx.doi.org/10.3390/biology5030032
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