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Cell-free synthesis of functional human epidermal growth factor receptor: Investigation of ligand-independent dimerization in Sf21 microsomal membranes using non-canonical amino acids
Cell-free protein synthesis systems represent versatile tools for the synthesis and modification of human membrane proteins. In particular, eukaryotic cell-free systems provide a promising platform for their structural and functional characterization. Here, we present the cell-free synthesis of func...
Autores principales: | Quast, Robert B., Ballion, Biljana, Stech, Marlitt, Sonnabend, Andrei, Varga, Balázs R., Wüstenhagen, Doreen A., Kele, Péter, Schiller, Stefan M., Kubick, Stefan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037433/ https://www.ncbi.nlm.nih.gov/pubmed/27670253 http://dx.doi.org/10.1038/srep34048 |
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