Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans

A novel Achromobacter xylosoxidans bacteriophage, phiAxp-2, was isolated from hospital sewage in China. The phage was morphologically and microbiologically characterized, and its one-step growth curve, host range, genomic sequence, and receptor were determined. Its morphology showed that phiAxp-2 be...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Erna, Yin, Zhe, Ma, Yanyan, Li, Huan, Lin, Weishi, Wei, Xiao, Zhao, Ruixiang, Jiang, Aimin, Yuan, Jing, Zhao, Xiangna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037462/
https://www.ncbi.nlm.nih.gov/pubmed/27669904
http://dx.doi.org/10.1038/srep34300
_version_ 1782455742629937152
author Li, Erna
Yin, Zhe
Ma, Yanyan
Li, Huan
Lin, Weishi
Wei, Xiao
Zhao, Ruixiang
Jiang, Aimin
Yuan, Jing
Zhao, Xiangna
author_facet Li, Erna
Yin, Zhe
Ma, Yanyan
Li, Huan
Lin, Weishi
Wei, Xiao
Zhao, Ruixiang
Jiang, Aimin
Yuan, Jing
Zhao, Xiangna
author_sort Li, Erna
collection PubMed
description A novel Achromobacter xylosoxidans bacteriophage, phiAxp-2, was isolated from hospital sewage in China. The phage was morphologically and microbiologically characterized, and its one-step growth curve, host range, genomic sequence, and receptor were determined. Its morphology showed that phiAxp-2 belongs to the family Siphoviridae. Microbiological characterization demonstrated that pH 7 is most suitable for phage phiAxp-2; its titer decreased when the temperature exceeded 50 °C; phiAxp-2 is sensitive to ethanol and isopropanol; and the presence of calcium and magnesium ions is necessary to accelerate cell lysis and improve the formation of phiAxp-2 plaques. Genomic sequencing and a bioinformatic analysis showed that phage phiAxp-2 is a novel bacteriophage, consisting of a circular, double-stranded 62,220-bp DNA molecule with a GC content of 60.11% that encodes 86 putative open reading frames (ORFs). The lipopolysaccharide of A. xylosoxidans is involved in the adsorption of phiAxp-2.
format Online
Article
Text
id pubmed-5037462
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-50374622016-09-30 Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans Li, Erna Yin, Zhe Ma, Yanyan Li, Huan Lin, Weishi Wei, Xiao Zhao, Ruixiang Jiang, Aimin Yuan, Jing Zhao, Xiangna Sci Rep Article A novel Achromobacter xylosoxidans bacteriophage, phiAxp-2, was isolated from hospital sewage in China. The phage was morphologically and microbiologically characterized, and its one-step growth curve, host range, genomic sequence, and receptor were determined. Its morphology showed that phiAxp-2 belongs to the family Siphoviridae. Microbiological characterization demonstrated that pH 7 is most suitable for phage phiAxp-2; its titer decreased when the temperature exceeded 50 °C; phiAxp-2 is sensitive to ethanol and isopropanol; and the presence of calcium and magnesium ions is necessary to accelerate cell lysis and improve the formation of phiAxp-2 plaques. Genomic sequencing and a bioinformatic analysis showed that phage phiAxp-2 is a novel bacteriophage, consisting of a circular, double-stranded 62,220-bp DNA molecule with a GC content of 60.11% that encodes 86 putative open reading frames (ORFs). The lipopolysaccharide of A. xylosoxidans is involved in the adsorption of phiAxp-2. Nature Publishing Group 2016-09-27 /pmc/articles/PMC5037462/ /pubmed/27669904 http://dx.doi.org/10.1038/srep34300 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Li, Erna
Yin, Zhe
Ma, Yanyan
Li, Huan
Lin, Weishi
Wei, Xiao
Zhao, Ruixiang
Jiang, Aimin
Yuan, Jing
Zhao, Xiangna
Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans
title Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans
title_full Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans
title_fullStr Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans
title_full_unstemmed Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans
title_short Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans
title_sort identification and molecular characterization of bacteriophage phiaxp-2 of achromobacter xylosoxidans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037462/
https://www.ncbi.nlm.nih.gov/pubmed/27669904
http://dx.doi.org/10.1038/srep34300
work_keys_str_mv AT lierna identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans
AT yinzhe identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans
AT mayanyan identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans
AT lihuan identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans
AT linweishi identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans
AT weixiao identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans
AT zhaoruixiang identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans
AT jiangaimin identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans
AT yuanjing identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans
AT zhaoxiangna identificationandmolecularcharacterizationofbacteriophagephiaxp2ofachromobacterxylosoxidans

Ejemplares similares