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Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk
The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was pe...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037494/ https://www.ncbi.nlm.nih.gov/pubmed/27649244 http://dx.doi.org/10.3390/toxins8090268 |
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author | Nia, Yacine Rodriguez, Mélanie Zeleny, Reinhard Herbin, Sabine Auvray, Frédéric Fiebig, Uwe Avondet, Marc-André Munoz, Amalia Hennekinne, Jacques-Antoine |
author_facet | Nia, Yacine Rodriguez, Mélanie Zeleny, Reinhard Herbin, Sabine Auvray, Frédéric Fiebig, Uwe Avondet, Marc-André Munoz, Amalia Hennekinne, Jacques-Antoine |
author_sort | Nia, Yacine |
collection | PubMed |
description | The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was performed using SDS-PAGE and SE-specific ELISA, and amino acid analysis was used to assign its protein concentration. The solution was then used to prepare six PT materials (four milk and two buffer batches) at a ng/g toxin level, which included one blank and one SEA-containing milk as specificity control. Suitable material homogeneity and stability were assessed using screening and quantitative ELISAs. Among the methods used by the participants, ELISA-based methods demonstrated their efficiency for the detection of SEB in both simple and complex matrices. The results serve as a basis for further improving the detection capabilities in expert laboratories and can therefore be considered as a contribution to biopreparedness. |
format | Online Article Text |
id | pubmed-5037494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-50374942016-09-29 Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk Nia, Yacine Rodriguez, Mélanie Zeleny, Reinhard Herbin, Sabine Auvray, Frédéric Fiebig, Uwe Avondet, Marc-André Munoz, Amalia Hennekinne, Jacques-Antoine Toxins (Basel) Article The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was performed using SDS-PAGE and SE-specific ELISA, and amino acid analysis was used to assign its protein concentration. The solution was then used to prepare six PT materials (four milk and two buffer batches) at a ng/g toxin level, which included one blank and one SEA-containing milk as specificity control. Suitable material homogeneity and stability were assessed using screening and quantitative ELISAs. Among the methods used by the participants, ELISA-based methods demonstrated their efficiency for the detection of SEB in both simple and complex matrices. The results serve as a basis for further improving the detection capabilities in expert laboratories and can therefore be considered as a contribution to biopreparedness. MDPI 2016-09-13 /pmc/articles/PMC5037494/ /pubmed/27649244 http://dx.doi.org/10.3390/toxins8090268 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nia, Yacine Rodriguez, Mélanie Zeleny, Reinhard Herbin, Sabine Auvray, Frédéric Fiebig, Uwe Avondet, Marc-André Munoz, Amalia Hennekinne, Jacques-Antoine Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_full | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_fullStr | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_full_unstemmed | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_short | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_sort | organization and elisa-based results of the first proficiency testing to evaluate the ability of european union laboratories to detect staphylococcal enterotoxin type b (seb) in buffer and milk |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037494/ https://www.ncbi.nlm.nih.gov/pubmed/27649244 http://dx.doi.org/10.3390/toxins8090268 |
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