Cargando…

Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus

In this study the effects of phosphate, potassium, yeast extract, and trace metals on the growth of Mucor indicus and chitosan, chitin, and metabolite production by the fungus were investigated. Maximum yield of chitosan (0.32 g/g cell wall) was obtained in a phosphate-free medium. Reversely, cell g...

Descripción completa

Detalles Bibliográficos
Autores principales: Safaei, Zahra, Karimi, Keikhosro, Zamani, Akram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037708/
https://www.ncbi.nlm.nih.gov/pubmed/27589726
http://dx.doi.org/10.3390/ijms17091429
_version_ 1782455796082147328
author Safaei, Zahra
Karimi, Keikhosro
Zamani, Akram
author_facet Safaei, Zahra
Karimi, Keikhosro
Zamani, Akram
author_sort Safaei, Zahra
collection PubMed
description In this study the effects of phosphate, potassium, yeast extract, and trace metals on the growth of Mucor indicus and chitosan, chitin, and metabolite production by the fungus were investigated. Maximum yield of chitosan (0.32 g/g cell wall) was obtained in a phosphate-free medium. Reversely, cell growth and ethanol formation by the fungus were positively affected in the presence of phosphate. In a phosphate-free medium, the highest chitosan content (0.42 g/g cell wall) and cell growth (0.66 g/g sugar) were obtained at 2.5 g/L of KOH. Potassium concentration had no significant effect on ethanol and glycerol yields. The presence of trace metals significantly increased the chitosan yield at an optimal phosphate and potassium concentration (0.50 g/g cell wall). By contrast, production of ethanol by the fungus was negatively affected (0.33 g/g sugars). A remarkable increase in chitin and decrease in chitosan were observed in the absence of yeast extract and concentrations lower than 2 g/L. The maximum chitosan yield of 51% cell wall was obtained at 5 g/L of yeast extract when the medium contained no phosphate, 2.5 g/L KOH, and 1 mL/L trace metal solution.
format Online
Article
Text
id pubmed-5037708
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-50377082016-09-29 Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus Safaei, Zahra Karimi, Keikhosro Zamani, Akram Int J Mol Sci Article In this study the effects of phosphate, potassium, yeast extract, and trace metals on the growth of Mucor indicus and chitosan, chitin, and metabolite production by the fungus were investigated. Maximum yield of chitosan (0.32 g/g cell wall) was obtained in a phosphate-free medium. Reversely, cell growth and ethanol formation by the fungus were positively affected in the presence of phosphate. In a phosphate-free medium, the highest chitosan content (0.42 g/g cell wall) and cell growth (0.66 g/g sugar) were obtained at 2.5 g/L of KOH. Potassium concentration had no significant effect on ethanol and glycerol yields. The presence of trace metals significantly increased the chitosan yield at an optimal phosphate and potassium concentration (0.50 g/g cell wall). By contrast, production of ethanol by the fungus was negatively affected (0.33 g/g sugars). A remarkable increase in chitin and decrease in chitosan were observed in the absence of yeast extract and concentrations lower than 2 g/L. The maximum chitosan yield of 51% cell wall was obtained at 5 g/L of yeast extract when the medium contained no phosphate, 2.5 g/L KOH, and 1 mL/L trace metal solution. MDPI 2016-08-30 /pmc/articles/PMC5037708/ /pubmed/27589726 http://dx.doi.org/10.3390/ijms17091429 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Safaei, Zahra
Karimi, Keikhosro
Zamani, Akram
Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus
title Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus
title_full Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus
title_fullStr Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus
title_full_unstemmed Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus
title_short Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus
title_sort impact of phosphate, potassium, yeast extract, and trace metals on chitosan and metabolite production by mucor indicus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037708/
https://www.ncbi.nlm.nih.gov/pubmed/27589726
http://dx.doi.org/10.3390/ijms17091429
work_keys_str_mv AT safaeizahra impactofphosphatepotassiumyeastextractandtracemetalsonchitosanandmetaboliteproductionbymucorindicus
AT karimikeikhosro impactofphosphatepotassiumyeastextractandtracemetalsonchitosanandmetaboliteproductionbymucorindicus
AT zamaniakram impactofphosphatepotassiumyeastextractandtracemetalsonchitosanandmetaboliteproductionbymucorindicus