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Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides

Ploidy affects plant growth vigor and cell size, but the relative effects of pollen fertility and allergenicity between triploid and diploid have not been systematically examined. Here we performed comparative analyses of fertility, proteome, and abundances of putative allergenic proteins of pollen...

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Autores principales: Zhang, Xiao-Ling, Zhang, Jin, Guo, Ying-Hua, Sun, Pei, Jia, Hui-Xia, Fan, Wei, Lu, Meng-Zhu, Hu, Jian-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037753/
https://www.ncbi.nlm.nih.gov/pubmed/27598155
http://dx.doi.org/10.3390/ijms17091475
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author Zhang, Xiao-Ling
Zhang, Jin
Guo, Ying-Hua
Sun, Pei
Jia, Hui-Xia
Fan, Wei
Lu, Meng-Zhu
Hu, Jian-Jun
author_facet Zhang, Xiao-Ling
Zhang, Jin
Guo, Ying-Hua
Sun, Pei
Jia, Hui-Xia
Fan, Wei
Lu, Meng-Zhu
Hu, Jian-Jun
author_sort Zhang, Xiao-Ling
collection PubMed
description Ploidy affects plant growth vigor and cell size, but the relative effects of pollen fertility and allergenicity between triploid and diploid have not been systematically examined. Here we performed comparative analyses of fertility, proteome, and abundances of putative allergenic proteins of pollen in triploid poplar ‘ZhongHuai1’ (‘ZH1’, triploid) and ‘ZhongHuai2’ (‘ZH2’, diploid) generated from the same parents. The mature pollen was sterile in triploid poplar ‘ZH1’. By applying two-dimensional gel electrophoresis (2-DE), a total of 72 differentially expressed protein spots (DEPs) were detected in triploid poplar pollen. Among them, 24 upregulated and 43 downregulated proteins were identified in triploid poplar pollen using matrix-assisted laser desorption/ionisation coupled with time of-flight tandem mass spectrometer analysis (MALDI-TOF/TOF MS/MS). The main functions of these DEPs were related with “S-adenosylmethionine metabolism”, “actin cytoskeleton organization”, or “translational elongation”. The infertility of triploid poplar pollen might be related to its abnormal cytoskeletal system. In addition, the abundances of previously identified 28 putative allergenic proteins were compared among three poplar varieties (‘ZH1’, ‘ZH2’, and ‘2KEN8‘). Most putative allergenic proteins were downregulated in triploid poplar pollen. This work provides an insight into understanding the protein regulation mechanism of pollen infertility and low allergenicity in triploid poplar, and gives a clue to improving poplar polyploidy breeding and decreasing the pollen allergenicity.
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spelling pubmed-50377532016-09-29 Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides Zhang, Xiao-Ling Zhang, Jin Guo, Ying-Hua Sun, Pei Jia, Hui-Xia Fan, Wei Lu, Meng-Zhu Hu, Jian-Jun Int J Mol Sci Article Ploidy affects plant growth vigor and cell size, but the relative effects of pollen fertility and allergenicity between triploid and diploid have not been systematically examined. Here we performed comparative analyses of fertility, proteome, and abundances of putative allergenic proteins of pollen in triploid poplar ‘ZhongHuai1’ (‘ZH1’, triploid) and ‘ZhongHuai2’ (‘ZH2’, diploid) generated from the same parents. The mature pollen was sterile in triploid poplar ‘ZH1’. By applying two-dimensional gel electrophoresis (2-DE), a total of 72 differentially expressed protein spots (DEPs) were detected in triploid poplar pollen. Among them, 24 upregulated and 43 downregulated proteins were identified in triploid poplar pollen using matrix-assisted laser desorption/ionisation coupled with time of-flight tandem mass spectrometer analysis (MALDI-TOF/TOF MS/MS). The main functions of these DEPs were related with “S-adenosylmethionine metabolism”, “actin cytoskeleton organization”, or “translational elongation”. The infertility of triploid poplar pollen might be related to its abnormal cytoskeletal system. In addition, the abundances of previously identified 28 putative allergenic proteins were compared among three poplar varieties (‘ZH1’, ‘ZH2’, and ‘2KEN8‘). Most putative allergenic proteins were downregulated in triploid poplar pollen. This work provides an insight into understanding the protein regulation mechanism of pollen infertility and low allergenicity in triploid poplar, and gives a clue to improving poplar polyploidy breeding and decreasing the pollen allergenicity. MDPI 2016-09-03 /pmc/articles/PMC5037753/ /pubmed/27598155 http://dx.doi.org/10.3390/ijms17091475 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Xiao-Ling
Zhang, Jin
Guo, Ying-Hua
Sun, Pei
Jia, Hui-Xia
Fan, Wei
Lu, Meng-Zhu
Hu, Jian-Jun
Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides
title Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides
title_full Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides
title_fullStr Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides
title_full_unstemmed Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides
title_short Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides
title_sort comparative proteomic analysis of mature pollen in triploid and diploid populus deltoides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037753/
https://www.ncbi.nlm.nih.gov/pubmed/27598155
http://dx.doi.org/10.3390/ijms17091475
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