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Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides
Ploidy affects plant growth vigor and cell size, but the relative effects of pollen fertility and allergenicity between triploid and diploid have not been systematically examined. Here we performed comparative analyses of fertility, proteome, and abundances of putative allergenic proteins of pollen...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037753/ https://www.ncbi.nlm.nih.gov/pubmed/27598155 http://dx.doi.org/10.3390/ijms17091475 |
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author | Zhang, Xiao-Ling Zhang, Jin Guo, Ying-Hua Sun, Pei Jia, Hui-Xia Fan, Wei Lu, Meng-Zhu Hu, Jian-Jun |
author_facet | Zhang, Xiao-Ling Zhang, Jin Guo, Ying-Hua Sun, Pei Jia, Hui-Xia Fan, Wei Lu, Meng-Zhu Hu, Jian-Jun |
author_sort | Zhang, Xiao-Ling |
collection | PubMed |
description | Ploidy affects plant growth vigor and cell size, but the relative effects of pollen fertility and allergenicity between triploid and diploid have not been systematically examined. Here we performed comparative analyses of fertility, proteome, and abundances of putative allergenic proteins of pollen in triploid poplar ‘ZhongHuai1’ (‘ZH1’, triploid) and ‘ZhongHuai2’ (‘ZH2’, diploid) generated from the same parents. The mature pollen was sterile in triploid poplar ‘ZH1’. By applying two-dimensional gel electrophoresis (2-DE), a total of 72 differentially expressed protein spots (DEPs) were detected in triploid poplar pollen. Among them, 24 upregulated and 43 downregulated proteins were identified in triploid poplar pollen using matrix-assisted laser desorption/ionisation coupled with time of-flight tandem mass spectrometer analysis (MALDI-TOF/TOF MS/MS). The main functions of these DEPs were related with “S-adenosylmethionine metabolism”, “actin cytoskeleton organization”, or “translational elongation”. The infertility of triploid poplar pollen might be related to its abnormal cytoskeletal system. In addition, the abundances of previously identified 28 putative allergenic proteins were compared among three poplar varieties (‘ZH1’, ‘ZH2’, and ‘2KEN8‘). Most putative allergenic proteins were downregulated in triploid poplar pollen. This work provides an insight into understanding the protein regulation mechanism of pollen infertility and low allergenicity in triploid poplar, and gives a clue to improving poplar polyploidy breeding and decreasing the pollen allergenicity. |
format | Online Article Text |
id | pubmed-5037753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-50377532016-09-29 Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides Zhang, Xiao-Ling Zhang, Jin Guo, Ying-Hua Sun, Pei Jia, Hui-Xia Fan, Wei Lu, Meng-Zhu Hu, Jian-Jun Int J Mol Sci Article Ploidy affects plant growth vigor and cell size, but the relative effects of pollen fertility and allergenicity between triploid and diploid have not been systematically examined. Here we performed comparative analyses of fertility, proteome, and abundances of putative allergenic proteins of pollen in triploid poplar ‘ZhongHuai1’ (‘ZH1’, triploid) and ‘ZhongHuai2’ (‘ZH2’, diploid) generated from the same parents. The mature pollen was sterile in triploid poplar ‘ZH1’. By applying two-dimensional gel electrophoresis (2-DE), a total of 72 differentially expressed protein spots (DEPs) were detected in triploid poplar pollen. Among them, 24 upregulated and 43 downregulated proteins were identified in triploid poplar pollen using matrix-assisted laser desorption/ionisation coupled with time of-flight tandem mass spectrometer analysis (MALDI-TOF/TOF MS/MS). The main functions of these DEPs were related with “S-adenosylmethionine metabolism”, “actin cytoskeleton organization”, or “translational elongation”. The infertility of triploid poplar pollen might be related to its abnormal cytoskeletal system. In addition, the abundances of previously identified 28 putative allergenic proteins were compared among three poplar varieties (‘ZH1’, ‘ZH2’, and ‘2KEN8‘). Most putative allergenic proteins were downregulated in triploid poplar pollen. This work provides an insight into understanding the protein regulation mechanism of pollen infertility and low allergenicity in triploid poplar, and gives a clue to improving poplar polyploidy breeding and decreasing the pollen allergenicity. MDPI 2016-09-03 /pmc/articles/PMC5037753/ /pubmed/27598155 http://dx.doi.org/10.3390/ijms17091475 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Xiao-Ling Zhang, Jin Guo, Ying-Hua Sun, Pei Jia, Hui-Xia Fan, Wei Lu, Meng-Zhu Hu, Jian-Jun Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides |
title | Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides |
title_full | Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides |
title_fullStr | Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides |
title_full_unstemmed | Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides |
title_short | Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides |
title_sort | comparative proteomic analysis of mature pollen in triploid and diploid populus deltoides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037753/ https://www.ncbi.nlm.nih.gov/pubmed/27598155 http://dx.doi.org/10.3390/ijms17091475 |
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