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Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea

Small GTPases play important roles in the growth, development and environmental responses of eukaryotes. Based on the genomic sequence of the straw mushroom Volvariella volvacea, 44 small GTPases were identified. A clustering analysis using human small GTPases as the references revealed that V. volv...

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Autores principales: Yan, Jun-Jie, Xie, Bin, Zhang, Lei, Li, Shao-Jie, van Peer, Arend F., Wu, Ta-Ju, Chen, Bing-Zhi, Xie, Bao-Gui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037802/
https://www.ncbi.nlm.nih.gov/pubmed/27626406
http://dx.doi.org/10.3390/ijms17091527
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author Yan, Jun-Jie
Xie, Bin
Zhang, Lei
Li, Shao-Jie
van Peer, Arend F.
Wu, Ta-Ju
Chen, Bing-Zhi
Xie, Bao-Gui
author_facet Yan, Jun-Jie
Xie, Bin
Zhang, Lei
Li, Shao-Jie
van Peer, Arend F.
Wu, Ta-Ju
Chen, Bing-Zhi
Xie, Bao-Gui
author_sort Yan, Jun-Jie
collection PubMed
description Small GTPases play important roles in the growth, development and environmental responses of eukaryotes. Based on the genomic sequence of the straw mushroom Volvariella volvacea, 44 small GTPases were identified. A clustering analysis using human small GTPases as the references revealed that V. volvacea small GTPases can be grouped into five families: nine are in the Ras family, 10 are in the Rho family, 15 are in the Rab family, one is in the Ran family and nine are in the Arf family. The transcription of vvran1 was up-regulated upon hydrogen peroxide (H(2)O(2)) stress, and could be repressed by diphenyleneiodonium chloride (DPI), a NADPH oxidase-specific inhibitor. The number of vvran1 transcripts also increased upon cold stress. Diphenyleneiodonium chloride, but not the superoxide dismutase (SOD) inhibitor diethy dithiocarbamate (DDC), could suppress the up-regulation of vvran1 gene expression to cold stress. These results combined with the high correlations between gene expression and superoxide anion (O(2)(−)) generation indicated that vvran1 could be one of the candidate genes in the downstream of O(2)(−) mediated pathways that are generated by NADPH oxidase under low temperature and oxidative stresses.
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spelling pubmed-50378022016-09-29 Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea Yan, Jun-Jie Xie, Bin Zhang, Lei Li, Shao-Jie van Peer, Arend F. Wu, Ta-Ju Chen, Bing-Zhi Xie, Bao-Gui Int J Mol Sci Article Small GTPases play important roles in the growth, development and environmental responses of eukaryotes. Based on the genomic sequence of the straw mushroom Volvariella volvacea, 44 small GTPases were identified. A clustering analysis using human small GTPases as the references revealed that V. volvacea small GTPases can be grouped into five families: nine are in the Ras family, 10 are in the Rho family, 15 are in the Rab family, one is in the Ran family and nine are in the Arf family. The transcription of vvran1 was up-regulated upon hydrogen peroxide (H(2)O(2)) stress, and could be repressed by diphenyleneiodonium chloride (DPI), a NADPH oxidase-specific inhibitor. The number of vvran1 transcripts also increased upon cold stress. Diphenyleneiodonium chloride, but not the superoxide dismutase (SOD) inhibitor diethy dithiocarbamate (DDC), could suppress the up-regulation of vvran1 gene expression to cold stress. These results combined with the high correlations between gene expression and superoxide anion (O(2)(−)) generation indicated that vvran1 could be one of the candidate genes in the downstream of O(2)(−) mediated pathways that are generated by NADPH oxidase under low temperature and oxidative stresses. MDPI 2016-09-10 /pmc/articles/PMC5037802/ /pubmed/27626406 http://dx.doi.org/10.3390/ijms17091527 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Jun-Jie
Xie, Bin
Zhang, Lei
Li, Shao-Jie
van Peer, Arend F.
Wu, Ta-Ju
Chen, Bing-Zhi
Xie, Bao-Gui
Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea
title Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea
title_full Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea
title_fullStr Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea
title_full_unstemmed Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea
title_short Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea
title_sort small gtpases and stress responses of vvran1 in the straw mushroom volvariella volvacea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037802/
https://www.ncbi.nlm.nih.gov/pubmed/27626406
http://dx.doi.org/10.3390/ijms17091527
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