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Identification of Bioactivity, Volatile and Fatty Acid Profile in Supercritical Fluid Extracts of Mexican arnica

Supercritical fluid extraction (SFE) is a sustainable technique used for the extraction of lipophilic metabolites such as pigments and fatty acids. Arnica plant is considered a potential candidate material with high antioxidant and antimicrobial activities. Therefore, in this study, a locally availa...

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Autores principales: García-Pérez, J. Saúl, Cuéllar-Bermúdez, Sara P., Arévalo-Gallegos, Alejandra, Rodríguez-Rodríguez, José, Iqbal, Hafiz M. N., Parra-Saldivar, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037803/
https://www.ncbi.nlm.nih.gov/pubmed/27626416
http://dx.doi.org/10.3390/ijms17091528
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author García-Pérez, J. Saúl
Cuéllar-Bermúdez, Sara P.
Arévalo-Gallegos, Alejandra
Rodríguez-Rodríguez, José
Iqbal, Hafiz M. N.
Parra-Saldivar, Roberto
author_facet García-Pérez, J. Saúl
Cuéllar-Bermúdez, Sara P.
Arévalo-Gallegos, Alejandra
Rodríguez-Rodríguez, José
Iqbal, Hafiz M. N.
Parra-Saldivar, Roberto
author_sort García-Pérez, J. Saúl
collection PubMed
description Supercritical fluid extraction (SFE) is a sustainable technique used for the extraction of lipophilic metabolites such as pigments and fatty acids. Arnica plant is considered a potential candidate material with high antioxidant and antimicrobial activities. Therefore, in this study, a locally available Heterotheca inuloides, also known as Mexican arnica, was analyzed for the extraction of high-value compounds. Based on different pressure (P), temperature (T), and co-solvent (CoS), four treatments (T) were prepared. A maximum 7.13% yield was recovered from T2 (T = 60 °C, P = 10 MPa, CoS = 8 g/min), followed by 6.69% from T4 (T = 60 °C, P = 30 MPa, CoS = 4 g/min). Some bioactive sesquiterpenoids such as 7-hydroxycadalene, caryophyllene and δ-cadinene were identified in the extracts by GC/MS. The fatty acid profile revealed that the main components were palmitic acid (C16:0), followed by linoleic acid (C18:2ω6c), α-linolenic acid (C18:3ω3) and stearic acid (C18:0) differing in percent yield per treatment. Antibacterial activities were determined by the agar diffusion method, indicating that all the treatments exerted strong antibacterial activity against S. aureus, C. albicans, and E. coli strains. The antioxidant capacity of the extracts was also measured by three in vitro assays, DPPH, TEAC and FRAP, using Trolox as a standard. Results showed high antioxidant capacity enabling pharmaceutical applications of Mexican arnica.
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spelling pubmed-50378032016-09-29 Identification of Bioactivity, Volatile and Fatty Acid Profile in Supercritical Fluid Extracts of Mexican arnica García-Pérez, J. Saúl Cuéllar-Bermúdez, Sara P. Arévalo-Gallegos, Alejandra Rodríguez-Rodríguez, José Iqbal, Hafiz M. N. Parra-Saldivar, Roberto Int J Mol Sci Article Supercritical fluid extraction (SFE) is a sustainable technique used for the extraction of lipophilic metabolites such as pigments and fatty acids. Arnica plant is considered a potential candidate material with high antioxidant and antimicrobial activities. Therefore, in this study, a locally available Heterotheca inuloides, also known as Mexican arnica, was analyzed for the extraction of high-value compounds. Based on different pressure (P), temperature (T), and co-solvent (CoS), four treatments (T) were prepared. A maximum 7.13% yield was recovered from T2 (T = 60 °C, P = 10 MPa, CoS = 8 g/min), followed by 6.69% from T4 (T = 60 °C, P = 30 MPa, CoS = 4 g/min). Some bioactive sesquiterpenoids such as 7-hydroxycadalene, caryophyllene and δ-cadinene were identified in the extracts by GC/MS. The fatty acid profile revealed that the main components were palmitic acid (C16:0), followed by linoleic acid (C18:2ω6c), α-linolenic acid (C18:3ω3) and stearic acid (C18:0) differing in percent yield per treatment. Antibacterial activities were determined by the agar diffusion method, indicating that all the treatments exerted strong antibacterial activity against S. aureus, C. albicans, and E. coli strains. The antioxidant capacity of the extracts was also measured by three in vitro assays, DPPH, TEAC and FRAP, using Trolox as a standard. Results showed high antioxidant capacity enabling pharmaceutical applications of Mexican arnica. MDPI 2016-09-12 /pmc/articles/PMC5037803/ /pubmed/27626416 http://dx.doi.org/10.3390/ijms17091528 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
García-Pérez, J. Saúl
Cuéllar-Bermúdez, Sara P.
Arévalo-Gallegos, Alejandra
Rodríguez-Rodríguez, José
Iqbal, Hafiz M. N.
Parra-Saldivar, Roberto
Identification of Bioactivity, Volatile and Fatty Acid Profile in Supercritical Fluid Extracts of Mexican arnica
title Identification of Bioactivity, Volatile and Fatty Acid Profile in Supercritical Fluid Extracts of Mexican arnica
title_full Identification of Bioactivity, Volatile and Fatty Acid Profile in Supercritical Fluid Extracts of Mexican arnica
title_fullStr Identification of Bioactivity, Volatile and Fatty Acid Profile in Supercritical Fluid Extracts of Mexican arnica
title_full_unstemmed Identification of Bioactivity, Volatile and Fatty Acid Profile in Supercritical Fluid Extracts of Mexican arnica
title_short Identification of Bioactivity, Volatile and Fatty Acid Profile in Supercritical Fluid Extracts of Mexican arnica
title_sort identification of bioactivity, volatile and fatty acid profile in supercritical fluid extracts of mexican arnica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037803/
https://www.ncbi.nlm.nih.gov/pubmed/27626416
http://dx.doi.org/10.3390/ijms17091528
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