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A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline
In vitro research in the field of type I diabetes is frequently limited by the availability of a functional model for islets of Langerhans. This method shows that by the addition of theophylline to the glucose buffers, mouse insulinoma MIN6 and rat insulinoma INS1E pseudo-islets can serve as a model...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037807/ https://www.ncbi.nlm.nih.gov/pubmed/27626415 http://dx.doi.org/10.3390/ijms17091532 |
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author | Groot Nibbelink, Milou Marchioli, Giulia Moroni, Lorenzo Karperien, Marcel Van Apeldoorn, Aart |
author_facet | Groot Nibbelink, Milou Marchioli, Giulia Moroni, Lorenzo Karperien, Marcel Van Apeldoorn, Aart |
author_sort | Groot Nibbelink, Milou |
collection | PubMed |
description | In vitro research in the field of type I diabetes is frequently limited by the availability of a functional model for islets of Langerhans. This method shows that by the addition of theophylline to the glucose buffers, mouse insulinoma MIN6 and rat insulinoma INS1E pseudo-islets can serve as a model for islets of Langerhans for in vitro research. The effect of theophylline is dose- and cell line-dependent, resulting in a minimal stimulation index of five followed by a rapid return to baseline insulin secretion by reducing glucose concentrations after a first high glucose stimulation. This protocol solves issues concerning in vitro research for type I diabetes as donors and the availability of primary islets of Langerhans are limited. To avoid the limitations of using human donor material, cell lines represent a valid alternative. Many different β cell lines have been reported, but the lack of reproducible responsiveness to glucose stimulation remains a challenge. |
format | Online Article Text |
id | pubmed-5037807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-50378072016-09-29 A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline Groot Nibbelink, Milou Marchioli, Giulia Moroni, Lorenzo Karperien, Marcel Van Apeldoorn, Aart Int J Mol Sci Communication In vitro research in the field of type I diabetes is frequently limited by the availability of a functional model for islets of Langerhans. This method shows that by the addition of theophylline to the glucose buffers, mouse insulinoma MIN6 and rat insulinoma INS1E pseudo-islets can serve as a model for islets of Langerhans for in vitro research. The effect of theophylline is dose- and cell line-dependent, resulting in a minimal stimulation index of five followed by a rapid return to baseline insulin secretion by reducing glucose concentrations after a first high glucose stimulation. This protocol solves issues concerning in vitro research for type I diabetes as donors and the availability of primary islets of Langerhans are limited. To avoid the limitations of using human donor material, cell lines represent a valid alternative. Many different β cell lines have been reported, but the lack of reproducible responsiveness to glucose stimulation remains a challenge. MDPI 2016-09-12 /pmc/articles/PMC5037807/ /pubmed/27626415 http://dx.doi.org/10.3390/ijms17091532 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Groot Nibbelink, Milou Marchioli, Giulia Moroni, Lorenzo Karperien, Marcel Van Apeldoorn, Aart A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline |
title | A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline |
title_full | A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline |
title_fullStr | A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline |
title_full_unstemmed | A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline |
title_short | A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline |
title_sort | protocol to enhance ins1e and min6 functionality—the use of theophylline |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037807/ https://www.ncbi.nlm.nih.gov/pubmed/27626415 http://dx.doi.org/10.3390/ijms17091532 |
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