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Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles

New approaches for visualisation of silicon nanoparticles (SiNPs) in cancer cells are realised by means of the linear and nonlinear optics in vitro. Aqueous colloidal solutions of SiNPs with sizes of about 10–40 nm obtained by ultrasound grinding of silicon nanowires were introduced into breast canc...

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Autores principales: Tolstik, Elen, Osminkina, Liubov A., Akimov, Denis, Gongalsky, Maksim B., Kudryavtsev, Andrew A., Timoshenko, Victor Yu., Heintzmann, Rainer, Sivakov, Vladimir, Popp, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037811/
https://www.ncbi.nlm.nih.gov/pubmed/27626408
http://dx.doi.org/10.3390/ijms17091536
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author Tolstik, Elen
Osminkina, Liubov A.
Akimov, Denis
Gongalsky, Maksim B.
Kudryavtsev, Andrew A.
Timoshenko, Victor Yu.
Heintzmann, Rainer
Sivakov, Vladimir
Popp, Jürgen
author_facet Tolstik, Elen
Osminkina, Liubov A.
Akimov, Denis
Gongalsky, Maksim B.
Kudryavtsev, Andrew A.
Timoshenko, Victor Yu.
Heintzmann, Rainer
Sivakov, Vladimir
Popp, Jürgen
author_sort Tolstik, Elen
collection PubMed
description New approaches for visualisation of silicon nanoparticles (SiNPs) in cancer cells are realised by means of the linear and nonlinear optics in vitro. Aqueous colloidal solutions of SiNPs with sizes of about 10–40 nm obtained by ultrasound grinding of silicon nanowires were introduced into breast cancer cells (MCF-7 cell line). Further, the time-varying nanoparticles enclosed in cell structures were visualised by high-resolution structured illumination microscopy (HR-SIM) and micro-Raman spectroscopy. Additionally, the nonlinear optical methods of two-photon excited fluorescence (TPEF) and coherent anti-Stokes Raman scattering (CARS) with infrared laser excitation were applied to study the localisation of SiNPs in cells. Advantages of the nonlinear methods, such as rapid imaging, which prevents cells from overheating and larger penetration depth compared to the single-photon excited HR-SIM, are discussed. The obtained results reveal new perspectives of the multimodal visualisation and precise detection of the uptake of biodegradable non-toxic SiNPs by cancer cells and they are discussed in view of future applications for the optical diagnostics of cancer tumours.
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spelling pubmed-50378112016-09-29 Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles Tolstik, Elen Osminkina, Liubov A. Akimov, Denis Gongalsky, Maksim B. Kudryavtsev, Andrew A. Timoshenko, Victor Yu. Heintzmann, Rainer Sivakov, Vladimir Popp, Jürgen Int J Mol Sci Article New approaches for visualisation of silicon nanoparticles (SiNPs) in cancer cells are realised by means of the linear and nonlinear optics in vitro. Aqueous colloidal solutions of SiNPs with sizes of about 10–40 nm obtained by ultrasound grinding of silicon nanowires were introduced into breast cancer cells (MCF-7 cell line). Further, the time-varying nanoparticles enclosed in cell structures were visualised by high-resolution structured illumination microscopy (HR-SIM) and micro-Raman spectroscopy. Additionally, the nonlinear optical methods of two-photon excited fluorescence (TPEF) and coherent anti-Stokes Raman scattering (CARS) with infrared laser excitation were applied to study the localisation of SiNPs in cells. Advantages of the nonlinear methods, such as rapid imaging, which prevents cells from overheating and larger penetration depth compared to the single-photon excited HR-SIM, are discussed. The obtained results reveal new perspectives of the multimodal visualisation and precise detection of the uptake of biodegradable non-toxic SiNPs by cancer cells and they are discussed in view of future applications for the optical diagnostics of cancer tumours. MDPI 2016-09-12 /pmc/articles/PMC5037811/ /pubmed/27626408 http://dx.doi.org/10.3390/ijms17091536 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tolstik, Elen
Osminkina, Liubov A.
Akimov, Denis
Gongalsky, Maksim B.
Kudryavtsev, Andrew A.
Timoshenko, Victor Yu.
Heintzmann, Rainer
Sivakov, Vladimir
Popp, Jürgen
Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles
title Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles
title_full Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles
title_fullStr Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles
title_full_unstemmed Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles
title_short Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles
title_sort linear and non-linear optical imaging of cancer cells with silicon nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037811/
https://www.ncbi.nlm.nih.gov/pubmed/27626408
http://dx.doi.org/10.3390/ijms17091536
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