Cargando…

De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris

Orchids are renowned for their spectacular flowers and ecological adaptations. After the sequencing of the genome of the tropical epiphytic orchid Phalaenopsis equestris, we combined Illumina HiSeq2000 for RNA-Seq and Trinity for de novo assembly to characterize the transcriptomes for 11 diverse P....

Descripción completa

Detalles Bibliográficos
Autores principales: Niu, Shan-Ce, Xu, Qing, Zhang, Guo-Qiang, Zhang, Yong-Qiang, Tsai, Wen-Chieh, Hsu, Jui-Ling, Liang, Chieh-Kai, Luo, Yi-Bo, Liu, Zhong-Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037975/
https://www.ncbi.nlm.nih.gov/pubmed/27673730
http://dx.doi.org/10.1038/sdata.2016.83
_version_ 1782455853238976512
author Niu, Shan-Ce
Xu, Qing
Zhang, Guo-Qiang
Zhang, Yong-Qiang
Tsai, Wen-Chieh
Hsu, Jui-Ling
Liang, Chieh-Kai
Luo, Yi-Bo
Liu, Zhong-Jian
author_facet Niu, Shan-Ce
Xu, Qing
Zhang, Guo-Qiang
Zhang, Yong-Qiang
Tsai, Wen-Chieh
Hsu, Jui-Ling
Liang, Chieh-Kai
Luo, Yi-Bo
Liu, Zhong-Jian
author_sort Niu, Shan-Ce
collection PubMed
description Orchids are renowned for their spectacular flowers and ecological adaptations. After the sequencing of the genome of the tropical epiphytic orchid Phalaenopsis equestris, we combined Illumina HiSeq2000 for RNA-Seq and Trinity for de novo assembly to characterize the transcriptomes for 11 diverse P. equestris tissues representing the root, stem, leaf, flower buds, column, lip, petal, sepal and three developmental stages of seeds. Our aims were to contribute to a better understanding of the molecular mechanisms driving the analysed tissue characteristics and to enrich the available data for P. equestris. Here, we present three databases. The first dataset is the RNA-Seq raw reads, which can be used to execute new experiments with different analysis approaches. The other two datasets allow different types of searches for candidate homologues. The second dataset includes the sets of assembled unigenes and predicted coding sequences and proteins, enabling a sequence-based search. The third dataset consists of the annotation results of the aligned unigenes versus the Nonredundant (Nr) protein database, Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) databases with low e-values, enabling a name-based search.
format Online
Article
Text
id pubmed-5037975
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-50379752016-10-04 De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris Niu, Shan-Ce Xu, Qing Zhang, Guo-Qiang Zhang, Yong-Qiang Tsai, Wen-Chieh Hsu, Jui-Ling Liang, Chieh-Kai Luo, Yi-Bo Liu, Zhong-Jian Sci Data Data Descriptor Orchids are renowned for their spectacular flowers and ecological adaptations. After the sequencing of the genome of the tropical epiphytic orchid Phalaenopsis equestris, we combined Illumina HiSeq2000 for RNA-Seq and Trinity for de novo assembly to characterize the transcriptomes for 11 diverse P. equestris tissues representing the root, stem, leaf, flower buds, column, lip, petal, sepal and three developmental stages of seeds. Our aims were to contribute to a better understanding of the molecular mechanisms driving the analysed tissue characteristics and to enrich the available data for P. equestris. Here, we present three databases. The first dataset is the RNA-Seq raw reads, which can be used to execute new experiments with different analysis approaches. The other two datasets allow different types of searches for candidate homologues. The second dataset includes the sets of assembled unigenes and predicted coding sequences and proteins, enabling a sequence-based search. The third dataset consists of the annotation results of the aligned unigenes versus the Nonredundant (Nr) protein database, Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) databases with low e-values, enabling a name-based search. Nature Publishing Group 2016-09-27 /pmc/articles/PMC5037975/ /pubmed/27673730 http://dx.doi.org/10.1038/sdata.2016.83 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse.
spellingShingle Data Descriptor
Niu, Shan-Ce
Xu, Qing
Zhang, Guo-Qiang
Zhang, Yong-Qiang
Tsai, Wen-Chieh
Hsu, Jui-Ling
Liang, Chieh-Kai
Luo, Yi-Bo
Liu, Zhong-Jian
De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
title De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
title_full De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
title_fullStr De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
title_full_unstemmed De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
title_short De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
title_sort de novo transcriptome assembly databases for the butterfly orchid phalaenopsis equestris
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037975/
https://www.ncbi.nlm.nih.gov/pubmed/27673730
http://dx.doi.org/10.1038/sdata.2016.83
work_keys_str_mv AT niushance denovotranscriptomeassemblydatabasesforthebutterflyorchidphalaenopsisequestris
AT xuqing denovotranscriptomeassemblydatabasesforthebutterflyorchidphalaenopsisequestris
AT zhangguoqiang denovotranscriptomeassemblydatabasesforthebutterflyorchidphalaenopsisequestris
AT zhangyongqiang denovotranscriptomeassemblydatabasesforthebutterflyorchidphalaenopsisequestris
AT tsaiwenchieh denovotranscriptomeassemblydatabasesforthebutterflyorchidphalaenopsisequestris
AT hsujuiling denovotranscriptomeassemblydatabasesforthebutterflyorchidphalaenopsisequestris
AT liangchiehkai denovotranscriptomeassemblydatabasesforthebutterflyorchidphalaenopsisequestris
AT luoyibo denovotranscriptomeassemblydatabasesforthebutterflyorchidphalaenopsisequestris
AT liuzhongjian denovotranscriptomeassemblydatabasesforthebutterflyorchidphalaenopsisequestris