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De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
Orchids are renowned for their spectacular flowers and ecological adaptations. After the sequencing of the genome of the tropical epiphytic orchid Phalaenopsis equestris, we combined Illumina HiSeq2000 for RNA-Seq and Trinity for de novo assembly to characterize the transcriptomes for 11 diverse P....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037975/ https://www.ncbi.nlm.nih.gov/pubmed/27673730 http://dx.doi.org/10.1038/sdata.2016.83 |
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author | Niu, Shan-Ce Xu, Qing Zhang, Guo-Qiang Zhang, Yong-Qiang Tsai, Wen-Chieh Hsu, Jui-Ling Liang, Chieh-Kai Luo, Yi-Bo Liu, Zhong-Jian |
author_facet | Niu, Shan-Ce Xu, Qing Zhang, Guo-Qiang Zhang, Yong-Qiang Tsai, Wen-Chieh Hsu, Jui-Ling Liang, Chieh-Kai Luo, Yi-Bo Liu, Zhong-Jian |
author_sort | Niu, Shan-Ce |
collection | PubMed |
description | Orchids are renowned for their spectacular flowers and ecological adaptations. After the sequencing of the genome of the tropical epiphytic orchid Phalaenopsis equestris, we combined Illumina HiSeq2000 for RNA-Seq and Trinity for de novo assembly to characterize the transcriptomes for 11 diverse P. equestris tissues representing the root, stem, leaf, flower buds, column, lip, petal, sepal and three developmental stages of seeds. Our aims were to contribute to a better understanding of the molecular mechanisms driving the analysed tissue characteristics and to enrich the available data for P. equestris. Here, we present three databases. The first dataset is the RNA-Seq raw reads, which can be used to execute new experiments with different analysis approaches. The other two datasets allow different types of searches for candidate homologues. The second dataset includes the sets of assembled unigenes and predicted coding sequences and proteins, enabling a sequence-based search. The third dataset consists of the annotation results of the aligned unigenes versus the Nonredundant (Nr) protein database, Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) databases with low e-values, enabling a name-based search. |
format | Online Article Text |
id | pubmed-5037975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50379752016-10-04 De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris Niu, Shan-Ce Xu, Qing Zhang, Guo-Qiang Zhang, Yong-Qiang Tsai, Wen-Chieh Hsu, Jui-Ling Liang, Chieh-Kai Luo, Yi-Bo Liu, Zhong-Jian Sci Data Data Descriptor Orchids are renowned for their spectacular flowers and ecological adaptations. After the sequencing of the genome of the tropical epiphytic orchid Phalaenopsis equestris, we combined Illumina HiSeq2000 for RNA-Seq and Trinity for de novo assembly to characterize the transcriptomes for 11 diverse P. equestris tissues representing the root, stem, leaf, flower buds, column, lip, petal, sepal and three developmental stages of seeds. Our aims were to contribute to a better understanding of the molecular mechanisms driving the analysed tissue characteristics and to enrich the available data for P. equestris. Here, we present three databases. The first dataset is the RNA-Seq raw reads, which can be used to execute new experiments with different analysis approaches. The other two datasets allow different types of searches for candidate homologues. The second dataset includes the sets of assembled unigenes and predicted coding sequences and proteins, enabling a sequence-based search. The third dataset consists of the annotation results of the aligned unigenes versus the Nonredundant (Nr) protein database, Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) databases with low e-values, enabling a name-based search. Nature Publishing Group 2016-09-27 /pmc/articles/PMC5037975/ /pubmed/27673730 http://dx.doi.org/10.1038/sdata.2016.83 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse. |
spellingShingle | Data Descriptor Niu, Shan-Ce Xu, Qing Zhang, Guo-Qiang Zhang, Yong-Qiang Tsai, Wen-Chieh Hsu, Jui-Ling Liang, Chieh-Kai Luo, Yi-Bo Liu, Zhong-Jian De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris |
title |
De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
|
title_full |
De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
|
title_fullStr |
De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
|
title_full_unstemmed |
De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
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title_short |
De novo transcriptome assembly databases for the butterfly orchid Phalaenopsis equestris
|
title_sort | de novo transcriptome assembly databases for the butterfly orchid phalaenopsis equestris |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5037975/ https://www.ncbi.nlm.nih.gov/pubmed/27673730 http://dx.doi.org/10.1038/sdata.2016.83 |
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