Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy

It has been proposed that cancer stem cells (CSCs) are involved in tumor resistance to chemotherapy and tumor relapse. The goal of the present study was to determine the effect of low-dose 5-fluorouracil (5-Fu) on enriched hepatocellular CSC-like cells. Increased cell motility and epithelial-mesench...

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Autores principales: Zhan, Yongqiang, Mou, Lisha, Cheng, Kangwen, Wang, Chengyou, Deng, Xuesong, Chen, Junren, Fan, Zhibing, Ni, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5038482/
https://www.ncbi.nlm.nih.gov/pubmed/27698820
http://dx.doi.org/10.3892/ol.2016.4975
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author Zhan, Yongqiang
Mou, Lisha
Cheng, Kangwen
Wang, Chengyou
Deng, Xuesong
Chen, Junren
Fan, Zhibing
Ni, Yong
author_facet Zhan, Yongqiang
Mou, Lisha
Cheng, Kangwen
Wang, Chengyou
Deng, Xuesong
Chen, Junren
Fan, Zhibing
Ni, Yong
author_sort Zhan, Yongqiang
collection PubMed
description It has been proposed that cancer stem cells (CSCs) are involved in tumor resistance to chemotherapy and tumor relapse. The goal of the present study was to determine the effect of low-dose 5-fluorouracil (5-Fu) on enriched hepatocellular CSC-like cells. Increased cell motility and epithelial-mesenchymal transition were observed by migration assay in human hepatoblastoma PLC/RAF/5 cells following 5-Fu treatment, as well as a significant enhancement in their sphere-forming abilities. CSC-like cells were identified by side population cell analysis. The percentage of CSC-like cells in the surviving cells was greatly increased in response to 5-Fu. These findings indicate that low-dose 5-Fu treatment may efficiently enrich the CSC-like cell population in PLC/RAF/5 cells.
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spelling pubmed-50384822016-10-03 Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy Zhan, Yongqiang Mou, Lisha Cheng, Kangwen Wang, Chengyou Deng, Xuesong Chen, Junren Fan, Zhibing Ni, Yong Oncol Lett Articles It has been proposed that cancer stem cells (CSCs) are involved in tumor resistance to chemotherapy and tumor relapse. The goal of the present study was to determine the effect of low-dose 5-fluorouracil (5-Fu) on enriched hepatocellular CSC-like cells. Increased cell motility and epithelial-mesenchymal transition were observed by migration assay in human hepatoblastoma PLC/RAF/5 cells following 5-Fu treatment, as well as a significant enhancement in their sphere-forming abilities. CSC-like cells were identified by side population cell analysis. The percentage of CSC-like cells in the surviving cells was greatly increased in response to 5-Fu. These findings indicate that low-dose 5-Fu treatment may efficiently enrich the CSC-like cell population in PLC/RAF/5 cells. D.A. Spandidos 2016-10 2016-08-08 /pmc/articles/PMC5038482/ /pubmed/27698820 http://dx.doi.org/10.3892/ol.2016.4975 Text en Copyright: © Zhan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhan, Yongqiang
Mou, Lisha
Cheng, Kangwen
Wang, Chengyou
Deng, Xuesong
Chen, Junren
Fan, Zhibing
Ni, Yong
Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy
title Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy
title_full Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy
title_fullStr Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy
title_full_unstemmed Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy
title_short Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy
title_sort hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5038482/
https://www.ncbi.nlm.nih.gov/pubmed/27698820
http://dx.doi.org/10.3892/ol.2016.4975
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