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Insights into autophagosome biogenesis from in vitro reconstitutions
Macro-autophagy (autophagy) is a conserved catabolic pathway for the degradation of cytoplasmic material in the lysosomal system. This is achieved by the sequestration of the cytoplasmic cargo material within double membrane-bound vesicles that fuse with lysosomes, wherein the vesicle’s inner membra...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academic Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039013/ https://www.ncbi.nlm.nih.gov/pubmed/27251905 http://dx.doi.org/10.1016/j.jsb.2016.04.005 |
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author | Turco, Eleonora Martens, Sascha |
author_facet | Turco, Eleonora Martens, Sascha |
author_sort | Turco, Eleonora |
collection | PubMed |
description | Macro-autophagy (autophagy) is a conserved catabolic pathway for the degradation of cytoplasmic material in the lysosomal system. This is achieved by the sequestration of the cytoplasmic cargo material within double membrane-bound vesicles that fuse with lysosomes, wherein the vesicle’s inner membrane and the cargo are degraded. Autophagosomes form in a de novo manner and their precursors are initially detected as small membrane structures that are referred to as isolation membranes. The isolation membranes gradually expand and subsequently close to give rise to autophagosomes. Many proteins required to form autophagosomes have been identified but how they act mechanistically is still enigmatic. Here we critically review reconstitution approaches employed to decipher the inner working of the fascinating autophagy machinery. |
format | Online Article Text |
id | pubmed-5039013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-50390132016-10-01 Insights into autophagosome biogenesis from in vitro reconstitutions Turco, Eleonora Martens, Sascha J Struct Biol Article Macro-autophagy (autophagy) is a conserved catabolic pathway for the degradation of cytoplasmic material in the lysosomal system. This is achieved by the sequestration of the cytoplasmic cargo material within double membrane-bound vesicles that fuse with lysosomes, wherein the vesicle’s inner membrane and the cargo are degraded. Autophagosomes form in a de novo manner and their precursors are initially detected as small membrane structures that are referred to as isolation membranes. The isolation membranes gradually expand and subsequently close to give rise to autophagosomes. Many proteins required to form autophagosomes have been identified but how they act mechanistically is still enigmatic. Here we critically review reconstitution approaches employed to decipher the inner working of the fascinating autophagy machinery. Academic Press 2016-10 /pmc/articles/PMC5039013/ /pubmed/27251905 http://dx.doi.org/10.1016/j.jsb.2016.04.005 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Turco, Eleonora Martens, Sascha Insights into autophagosome biogenesis from in vitro reconstitutions |
title | Insights into autophagosome biogenesis from in vitro reconstitutions |
title_full | Insights into autophagosome biogenesis from in vitro reconstitutions |
title_fullStr | Insights into autophagosome biogenesis from in vitro reconstitutions |
title_full_unstemmed | Insights into autophagosome biogenesis from in vitro reconstitutions |
title_short | Insights into autophagosome biogenesis from in vitro reconstitutions |
title_sort | insights into autophagosome biogenesis from in vitro reconstitutions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039013/ https://www.ncbi.nlm.nih.gov/pubmed/27251905 http://dx.doi.org/10.1016/j.jsb.2016.04.005 |
work_keys_str_mv | AT turcoeleonora insightsintoautophagosomebiogenesisfrominvitroreconstitutions AT martenssascha insightsintoautophagosomebiogenesisfrominvitroreconstitutions |