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Dynamics of a Class 1 Integron Located on Plasmid or Chromosome in Two Aeromonas spp. Strains

Integrons are non-mobile bacterial genetic elements that carry different cassettes conferring antibiotic resistance. Cassettes can excise or integrate by action of an integron-encoded integrase, enabling bacteria to face environmental challenges. In this work, the functionality and dynamics of two i...

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Autores principales: Pérez-Valdespino, Abigail, Lazarini-Martínez, Alfredo, Rivera-González, Alejandro X., García-Hernández, Normand, Curiel-Quesada, Everardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039178/
https://www.ncbi.nlm.nih.gov/pubmed/27733851
http://dx.doi.org/10.3389/fmicb.2016.01556
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author Pérez-Valdespino, Abigail
Lazarini-Martínez, Alfredo
Rivera-González, Alejandro X.
García-Hernández, Normand
Curiel-Quesada, Everardo
author_facet Pérez-Valdespino, Abigail
Lazarini-Martínez, Alfredo
Rivera-González, Alejandro X.
García-Hernández, Normand
Curiel-Quesada, Everardo
author_sort Pérez-Valdespino, Abigail
collection PubMed
description Integrons are non-mobile bacterial genetic elements that carry different cassettes conferring antibiotic resistance. Cassettes can excise or integrate by action of an integron-encoded integrase, enabling bacteria to face environmental challenges. In this work, the functionality and dynamics of two integrons carrying the same cassette arrangement (dfrA12–orfF–aadA2), but located on plasmid or chromosome in two different strains were studied. In order to demonstrate the functionality of the Class 1 integrase, circular cassette integration intermediaries were PCR amplified by PCR using extrachromosomal DNA extracted from bacteria grown in the presence or absence of cassette-encoded antibiotics. Circular aadA2 and dfrA12–orfF–aadA2 cassettes were detected in cultures grown either in the presence or absence of antibiotics in both strains. No dfrA12–orfF circular intermediates could be detected under any culture conditions. These results show that both integrons are functional. However, these elements show different dynamics and functionality since the presence of streptomycin led to detectable gene rearrangements in the variable region only in the strain with the plasmid-born integron. In addition, complete integration products were demonstrated using a receptor molecule carrying an empty integron. In this case, integration products were observed in both strains even in the absence of antibiotics, but they were more evident in the strain with the plasmid-located integron when streptomycin was present in the culture medium. This suggests that integrons in the two strains respond differently to streptomycin even though DNA sequences upstream the intI1 gene, including the lexA boxes of both integrons are identical.
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spelling pubmed-50391782016-10-12 Dynamics of a Class 1 Integron Located on Plasmid or Chromosome in Two Aeromonas spp. Strains Pérez-Valdespino, Abigail Lazarini-Martínez, Alfredo Rivera-González, Alejandro X. García-Hernández, Normand Curiel-Quesada, Everardo Front Microbiol Microbiology Integrons are non-mobile bacterial genetic elements that carry different cassettes conferring antibiotic resistance. Cassettes can excise or integrate by action of an integron-encoded integrase, enabling bacteria to face environmental challenges. In this work, the functionality and dynamics of two integrons carrying the same cassette arrangement (dfrA12–orfF–aadA2), but located on plasmid or chromosome in two different strains were studied. In order to demonstrate the functionality of the Class 1 integrase, circular cassette integration intermediaries were PCR amplified by PCR using extrachromosomal DNA extracted from bacteria grown in the presence or absence of cassette-encoded antibiotics. Circular aadA2 and dfrA12–orfF–aadA2 cassettes were detected in cultures grown either in the presence or absence of antibiotics in both strains. No dfrA12–orfF circular intermediates could be detected under any culture conditions. These results show that both integrons are functional. However, these elements show different dynamics and functionality since the presence of streptomycin led to detectable gene rearrangements in the variable region only in the strain with the plasmid-born integron. In addition, complete integration products were demonstrated using a receptor molecule carrying an empty integron. In this case, integration products were observed in both strains even in the absence of antibiotics, but they were more evident in the strain with the plasmid-located integron when streptomycin was present in the culture medium. This suggests that integrons in the two strains respond differently to streptomycin even though DNA sequences upstream the intI1 gene, including the lexA boxes of both integrons are identical. Frontiers Media S.A. 2016-09-28 /pmc/articles/PMC5039178/ /pubmed/27733851 http://dx.doi.org/10.3389/fmicb.2016.01556 Text en Copyright © 2016 Pérez-Valdespino, Lazarini-Martínez, Rivera-González, García-Hernández and Curiel-Quesada. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Pérez-Valdespino, Abigail
Lazarini-Martínez, Alfredo
Rivera-González, Alejandro X.
García-Hernández, Normand
Curiel-Quesada, Everardo
Dynamics of a Class 1 Integron Located on Plasmid or Chromosome in Two Aeromonas spp. Strains
title Dynamics of a Class 1 Integron Located on Plasmid or Chromosome in Two Aeromonas spp. Strains
title_full Dynamics of a Class 1 Integron Located on Plasmid or Chromosome in Two Aeromonas spp. Strains
title_fullStr Dynamics of a Class 1 Integron Located on Plasmid or Chromosome in Two Aeromonas spp. Strains
title_full_unstemmed Dynamics of a Class 1 Integron Located on Plasmid or Chromosome in Two Aeromonas spp. Strains
title_short Dynamics of a Class 1 Integron Located on Plasmid or Chromosome in Two Aeromonas spp. Strains
title_sort dynamics of a class 1 integron located on plasmid or chromosome in two aeromonas spp. strains
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039178/
https://www.ncbi.nlm.nih.gov/pubmed/27733851
http://dx.doi.org/10.3389/fmicb.2016.01556
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