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Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array

Prokaryotic adaptive immune systems are composed of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins. These systems adapt to new threats by integrating short nucleic acids, termed spacers, into the CRISPR array. The functional motifs in the repe...

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Autores principales: Goren, Moran G., Doron, Shany, Globus, Rea, Amitai, Gil, Sorek, Rotem, Qimron, Udi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039180/
https://www.ncbi.nlm.nih.gov/pubmed/27626652
http://dx.doi.org/10.1016/j.celrep.2016.08.043
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author Goren, Moran G.
Doron, Shany
Globus, Rea
Amitai, Gil
Sorek, Rotem
Qimron, Udi
author_facet Goren, Moran G.
Doron, Shany
Globus, Rea
Amitai, Gil
Sorek, Rotem
Qimron, Udi
author_sort Goren, Moran G.
collection PubMed
description Prokaryotic adaptive immune systems are composed of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins. These systems adapt to new threats by integrating short nucleic acids, termed spacers, into the CRISPR array. The functional motifs in the repeat and the mechanism by which a constant repeat size is maintained are still elusive. Here, through a series of mutations within the repeat of the CRISPR-Cas type I-E, we identify motifs that are crucial for adaptation and show that they serve as anchor sites for two molecular rulers determining the size of the new repeat. Adaptation products from various repeat mutants support a model in which two motifs in the repeat bind to two different sites in the adaptation complex that are 8 and 16 bp away from the active site. This model significantly extends our understanding of the adaptation process and broadens the scope of its applications.
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spelling pubmed-50391802016-09-30 Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array Goren, Moran G. Doron, Shany Globus, Rea Amitai, Gil Sorek, Rotem Qimron, Udi Cell Rep Report Prokaryotic adaptive immune systems are composed of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins. These systems adapt to new threats by integrating short nucleic acids, termed spacers, into the CRISPR array. The functional motifs in the repeat and the mechanism by which a constant repeat size is maintained are still elusive. Here, through a series of mutations within the repeat of the CRISPR-Cas type I-E, we identify motifs that are crucial for adaptation and show that they serve as anchor sites for two molecular rulers determining the size of the new repeat. Adaptation products from various repeat mutants support a model in which two motifs in the repeat bind to two different sites in the adaptation complex that are 8 and 16 bp away from the active site. This model significantly extends our understanding of the adaptation process and broadens the scope of its applications. Cell Press 2016-09-13 /pmc/articles/PMC5039180/ /pubmed/27626652 http://dx.doi.org/10.1016/j.celrep.2016.08.043 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
Goren, Moran G.
Doron, Shany
Globus, Rea
Amitai, Gil
Sorek, Rotem
Qimron, Udi
Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array
title Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array
title_full Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array
title_fullStr Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array
title_full_unstemmed Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array
title_short Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array
title_sort repeat size determination by two molecular rulers in the type i-e crispr array
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039180/
https://www.ncbi.nlm.nih.gov/pubmed/27626652
http://dx.doi.org/10.1016/j.celrep.2016.08.043
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