(1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1

TOCA1 is a downstream effector protein of the small GTPase, Cdc42. It is a multi-domain protein that includes a membrane binding F-BAR domain, a homology region 1 (HR1) domain, which binds selectively to active Cdc42 and an SH3 domain. TOCA1 is involved in the regulation of actin dynamics in process...

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Autores principales: Watson, Joanna R., Nietlispach, Daniel, Owen, Darerca, Mott, Helen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039218/
https://www.ncbi.nlm.nih.gov/pubmed/26988723
http://dx.doi.org/10.1007/s12104-016-9677-8
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author Watson, Joanna R.
Nietlispach, Daniel
Owen, Darerca
Mott, Helen R.
author_facet Watson, Joanna R.
Nietlispach, Daniel
Owen, Darerca
Mott, Helen R.
author_sort Watson, Joanna R.
collection PubMed
description TOCA1 is a downstream effector protein of the small GTPase, Cdc42. It is a multi-domain protein that includes a membrane binding F-BAR domain, a homology region 1 (HR1) domain, which binds selectively to active Cdc42 and an SH3 domain. TOCA1 is involved in the regulation of actin dynamics in processes such as endocytosis, filopodia formation, neurite elongation, cell motility and invasion. Structural insight into the interaction between TOCA1 and Cdc42 will contribute to our understanding of the role of TOCA1 in actin dynamics. The (1)H, (15)N and (13)C NMR backbone and sidechain resonance assignment of the HR1 domain (12 kDa) presented here provides the foundation for structural studies of the domain and its interactions.
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spelling pubmed-50392182016-10-11 (1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1 Watson, Joanna R. Nietlispach, Daniel Owen, Darerca Mott, Helen R. Biomol NMR Assign Article TOCA1 is a downstream effector protein of the small GTPase, Cdc42. It is a multi-domain protein that includes a membrane binding F-BAR domain, a homology region 1 (HR1) domain, which binds selectively to active Cdc42 and an SH3 domain. TOCA1 is involved in the regulation of actin dynamics in processes such as endocytosis, filopodia formation, neurite elongation, cell motility and invasion. Structural insight into the interaction between TOCA1 and Cdc42 will contribute to our understanding of the role of TOCA1 in actin dynamics. The (1)H, (15)N and (13)C NMR backbone and sidechain resonance assignment of the HR1 domain (12 kDa) presented here provides the foundation for structural studies of the domain and its interactions. Springer Netherlands 2016-03-18 2016 /pmc/articles/PMC5039218/ /pubmed/26988723 http://dx.doi.org/10.1007/s12104-016-9677-8 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Watson, Joanna R.
Nietlispach, Daniel
Owen, Darerca
Mott, Helen R.
(1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1
title (1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1
title_full (1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1
title_fullStr (1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1
title_full_unstemmed (1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1
title_short (1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1
title_sort (1)h, (13)c and (15)n resonance assignments of the cdc42-binding domain of toca1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039218/
https://www.ncbi.nlm.nih.gov/pubmed/26988723
http://dx.doi.org/10.1007/s12104-016-9677-8
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