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Comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders

OBJECTIVE: This study was conducted to compare the effects of static culture, dynamic culture, and the combination of dynamic culture with specialized surfaces involving co-culture on human embryonic development. Embryos cultured using conventional static culture (SC) techniques served as a control...

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Main Authors: Hur, Yong Soo, Ryu, Eun Kyung, Yoon, San Hyun, Lim, Kyung Sil, Lee, Won Don, Lim, Jin Ho
Format: Online Article Text
Language:English
Published: The Korean Society for Reproductive Medicine 2016
Subjects:
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039306/
https://www.ncbi.nlm.nih.gov/pubmed/27689036
http://dx.doi.org/10.5653/cerm.2016.43.3.146
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author Hur, Yong Soo
Ryu, Eun Kyung
Yoon, San Hyun
Lim, Kyung Sil
Lee, Won Don
Lim, Jin Ho
author_facet Hur, Yong Soo
Ryu, Eun Kyung
Yoon, San Hyun
Lim, Kyung Sil
Lee, Won Don
Lim, Jin Ho
author_sort Hur, Yong Soo
collection PubMed
description OBJECTIVE: This study was conducted to compare the effects of static culture, dynamic culture, and the combination of dynamic culture with specialized surfaces involving co-culture on human embryonic development. Embryos cultured using conventional static culture (SC) techniques served as a control group. We compared dynamic culture using micro-vibration culture (MVC) and micro-vibration with co-culture (MCoC), in which autologous cumulus cells were used as a specialized surface. METHODS: We conducted a chart review of patients who were treated between January 2011 and November 2014 in order to compare embryonic development rates and pregnancy rates among the groups. Zygotes were cultured in micro-droplets, and embryos were subsequently selected for transfer. Some surplus embryos were cryopreserved, and the others were cultured for blastocyst development. A micro-vibrator was set at the frequency of 42 Hz for duration of 5 seconds per 60 minutes to facilitate embryo development. RESULTS: No significant differences among the groups were present in patient's characteristics. However, the clinical pregnancy rates were significantly higher in the MVC group and the MCoC group than in the SC group. No significant differences were found in the blastocyst development rate between the SC group and the MVC group, but the blastocyst development rate in the MCoC group was significantly higher than in the SC and MVC groups. CONCLUSION: The clinical pregnancy rate was significantly increased by the application of micro-vibration to the embryonic cultures of poor responders. The blastocyst development rate was significantly increased by the application of MCoC to surplus embryos.
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spelling pubmed-50393062016-09-29 Comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders Hur, Yong Soo Ryu, Eun Kyung Yoon, San Hyun Lim, Kyung Sil Lee, Won Don Lim, Jin Ho Clin Exp Reprod Med Original Article OBJECTIVE: This study was conducted to compare the effects of static culture, dynamic culture, and the combination of dynamic culture with specialized surfaces involving co-culture on human embryonic development. Embryos cultured using conventional static culture (SC) techniques served as a control group. We compared dynamic culture using micro-vibration culture (MVC) and micro-vibration with co-culture (MCoC), in which autologous cumulus cells were used as a specialized surface. METHODS: We conducted a chart review of patients who were treated between January 2011 and November 2014 in order to compare embryonic development rates and pregnancy rates among the groups. Zygotes were cultured in micro-droplets, and embryos were subsequently selected for transfer. Some surplus embryos were cryopreserved, and the others were cultured for blastocyst development. A micro-vibrator was set at the frequency of 42 Hz for duration of 5 seconds per 60 minutes to facilitate embryo development. RESULTS: No significant differences among the groups were present in patient's characteristics. However, the clinical pregnancy rates were significantly higher in the MVC group and the MCoC group than in the SC group. No significant differences were found in the blastocyst development rate between the SC group and the MVC group, but the blastocyst development rate in the MCoC group was significantly higher than in the SC and MVC groups. CONCLUSION: The clinical pregnancy rate was significantly increased by the application of micro-vibration to the embryonic cultures of poor responders. The blastocyst development rate was significantly increased by the application of MCoC to surplus embryos. The Korean Society for Reproductive Medicine 2016-09 2016-09-22 /pmc/articles/PMC5039306/ /pubmed/27689036 http://dx.doi.org/10.5653/cerm.2016.43.3.146 Text en Copyright © 2016. The Korean Society for Reproductive Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hur, Yong Soo
Ryu, Eun Kyung
Yoon, San Hyun
Lim, Kyung Sil
Lee, Won Don
Lim, Jin Ho
Comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders
title Comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders
title_full Comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders
title_fullStr Comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders
title_full_unstemmed Comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders
title_short Comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders
title_sort comparison of static culture, micro-vibration culture, and micro-vibration culture with co-culture in poor ovarian responders
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039306/
https://www.ncbi.nlm.nih.gov/pubmed/27689036
http://dx.doi.org/10.5653/cerm.2016.43.3.146
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