The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers

OBJECTIVE: We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. METHODS: Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona opening performed at the site of th...

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Autores principales: Sanmee, Usanee, Piromlertamorn, Waraporn, Vutyavanich, Teraporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Reproductive Medicine 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039307/
https://www.ncbi.nlm.nih.gov/pubmed/27689037
http://dx.doi.org/10.5653/cerm.2016.43.3.152
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author Sanmee, Usanee
Piromlertamorn, Waraporn
Vutyavanich, Teraporn
author_facet Sanmee, Usanee
Piromlertamorn, Waraporn
Vutyavanich, Teraporn
author_sort Sanmee, Usanee
collection PubMed
description OBJECTIVE: We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. METHODS: Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona opening performed at the site of the ICM, n=125), the trophectoderm (TE) group (zona opening performed opposite to the ICM, n=125) and the control group (no zona opening, n=125). RESULTS: The rate of complete hatching of the blastocysts was not significantly different in the ICM and the TE group (84.8% vs 80.8%, respectively; p=0.402), but was significantly lower in the control group (51.2%, p<0.001). The cell numbers in the completely hatched blastocysts were comparable in the control group, the ICM group, and the TE group (69±19.3, 74±15.7, and 71±16.8, respectively; p=0.680). CONCLUSION: These findings indicate that the site of laser zona opening did not influence the rate of complete hatching of mouse blastocysts or their cell numbers.
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spelling pubmed-50393072016-09-29 The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers Sanmee, Usanee Piromlertamorn, Waraporn Vutyavanich, Teraporn Clin Exp Reprod Med Original Article OBJECTIVE: We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. METHODS: Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona opening performed at the site of the ICM, n=125), the trophectoderm (TE) group (zona opening performed opposite to the ICM, n=125) and the control group (no zona opening, n=125). RESULTS: The rate of complete hatching of the blastocysts was not significantly different in the ICM and the TE group (84.8% vs 80.8%, respectively; p=0.402), but was significantly lower in the control group (51.2%, p<0.001). The cell numbers in the completely hatched blastocysts were comparable in the control group, the ICM group, and the TE group (69±19.3, 74±15.7, and 71±16.8, respectively; p=0.680). CONCLUSION: These findings indicate that the site of laser zona opening did not influence the rate of complete hatching of mouse blastocysts or their cell numbers. The Korean Society for Reproductive Medicine 2016-09 2016-09-22 /pmc/articles/PMC5039307/ /pubmed/27689037 http://dx.doi.org/10.5653/cerm.2016.43.3.152 Text en Copyright © 2016. The Korean Society for Reproductive Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sanmee, Usanee
Piromlertamorn, Waraporn
Vutyavanich, Teraporn
The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers
title The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers
title_full The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers
title_fullStr The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers
title_full_unstemmed The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers
title_short The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers
title_sort effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039307/
https://www.ncbi.nlm.nih.gov/pubmed/27689037
http://dx.doi.org/10.5653/cerm.2016.43.3.152
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