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MicroRNA Expression Profile on Solid Subtype of Invasive Lung Adenocarcinoma Reveals a Panel of Four miRNAs to Be Associated with Poor Prognosis in Chinese Patients

According to the reclassification of lung adenocarcinoma (LAC) proposed in 2011, solid predominant lung adenocarcinoma (SPA) has been associated with poor outcomes for LAC patients. However, the prognostic value of the presence of solid subtype remains unclear. Besides, there is little data about th...

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Autores principales: Zhang, Yong-Qiang, Wang, Wei-Ya, Xue, Jian-Xin, Xu, Yan, Fan, Ping, Caughey, Bennett Adam, Tan, Wei-Wei, Cao, Gui-Qun, Jiang, Li-Li, Lu, You, Zhang, Kang, Hu, Xun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039382/
https://www.ncbi.nlm.nih.gov/pubmed/27698898
http://dx.doi.org/10.7150/jca.14923
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author Zhang, Yong-Qiang
Wang, Wei-Ya
Xue, Jian-Xin
Xu, Yan
Fan, Ping
Caughey, Bennett Adam
Tan, Wei-Wei
Cao, Gui-Qun
Jiang, Li-Li
Lu, You
Zhang, Kang
Hu, Xun
author_facet Zhang, Yong-Qiang
Wang, Wei-Ya
Xue, Jian-Xin
Xu, Yan
Fan, Ping
Caughey, Bennett Adam
Tan, Wei-Wei
Cao, Gui-Qun
Jiang, Li-Li
Lu, You
Zhang, Kang
Hu, Xun
author_sort Zhang, Yong-Qiang
collection PubMed
description According to the reclassification of lung adenocarcinoma (LAC) proposed in 2011, solid predominant lung adenocarcinoma (SPA) has been associated with poor outcomes for LAC patients. However, the prognostic value of the presence of solid subtype remains unclear. Besides, there is little data about the roles of microRNA (miRNA) in solid subtype of LAC. In this study, 243 LAC patients were classified into solid subtype positive and negative groups (S+ LAC, n=134 and S- LAC, n=109) according to whether the solid subtype was more than 5% of the tumor component or not. We analyzed the relationship between solid subtype and patients' outcome by univariate and multivariate analyses. Solid subtype was proved to be significantly associated with the 5-year overall survival and played as an independent prognostic factor for stage I-III invasive LAC patients. Then miRNA microarray was used to identify differentially expressed miRNAs in solid subtype, resulting in 31 differential miRNAs. Quantitative reverse transcription-PCR (QRT-PCR) was used to validate 4 key miRNAs (miR-133b, miR-155-5p, miR-124-3p, miR-145-5p). Further, CCK-8 and transwell assays were performed to validate the impact of one dysregulated miRNA (miR-133b) on LAC cell function. Interestingly, while miR-133b could significantly inhibit the proliferation of A549 and SPC-A1, it showed no effect on the migration or invasion of LAC cell lines. These results suggest that solid subtype can exert independent prognostic impact on LAC patients, and 4 important dysregulated miRNAs in solid subtype of LAC may be involved in the malignancy of S+LAC, thus may further have clinical perspective for S+ LAC in the future.
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spelling pubmed-50393822016-10-03 MicroRNA Expression Profile on Solid Subtype of Invasive Lung Adenocarcinoma Reveals a Panel of Four miRNAs to Be Associated with Poor Prognosis in Chinese Patients Zhang, Yong-Qiang Wang, Wei-Ya Xue, Jian-Xin Xu, Yan Fan, Ping Caughey, Bennett Adam Tan, Wei-Wei Cao, Gui-Qun Jiang, Li-Li Lu, You Zhang, Kang Hu, Xun J Cancer Research Paper According to the reclassification of lung adenocarcinoma (LAC) proposed in 2011, solid predominant lung adenocarcinoma (SPA) has been associated with poor outcomes for LAC patients. However, the prognostic value of the presence of solid subtype remains unclear. Besides, there is little data about the roles of microRNA (miRNA) in solid subtype of LAC. In this study, 243 LAC patients were classified into solid subtype positive and negative groups (S+ LAC, n=134 and S- LAC, n=109) according to whether the solid subtype was more than 5% of the tumor component or not. We analyzed the relationship between solid subtype and patients' outcome by univariate and multivariate analyses. Solid subtype was proved to be significantly associated with the 5-year overall survival and played as an independent prognostic factor for stage I-III invasive LAC patients. Then miRNA microarray was used to identify differentially expressed miRNAs in solid subtype, resulting in 31 differential miRNAs. Quantitative reverse transcription-PCR (QRT-PCR) was used to validate 4 key miRNAs (miR-133b, miR-155-5p, miR-124-3p, miR-145-5p). Further, CCK-8 and transwell assays were performed to validate the impact of one dysregulated miRNA (miR-133b) on LAC cell function. Interestingly, while miR-133b could significantly inhibit the proliferation of A549 and SPC-A1, it showed no effect on the migration or invasion of LAC cell lines. These results suggest that solid subtype can exert independent prognostic impact on LAC patients, and 4 important dysregulated miRNAs in solid subtype of LAC may be involved in the malignancy of S+LAC, thus may further have clinical perspective for S+ LAC in the future. Ivyspring International Publisher 2016-07-18 /pmc/articles/PMC5039382/ /pubmed/27698898 http://dx.doi.org/10.7150/jca.14923 Text en © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.
spellingShingle Research Paper
Zhang, Yong-Qiang
Wang, Wei-Ya
Xue, Jian-Xin
Xu, Yan
Fan, Ping
Caughey, Bennett Adam
Tan, Wei-Wei
Cao, Gui-Qun
Jiang, Li-Li
Lu, You
Zhang, Kang
Hu, Xun
MicroRNA Expression Profile on Solid Subtype of Invasive Lung Adenocarcinoma Reveals a Panel of Four miRNAs to Be Associated with Poor Prognosis in Chinese Patients
title MicroRNA Expression Profile on Solid Subtype of Invasive Lung Adenocarcinoma Reveals a Panel of Four miRNAs to Be Associated with Poor Prognosis in Chinese Patients
title_full MicroRNA Expression Profile on Solid Subtype of Invasive Lung Adenocarcinoma Reveals a Panel of Four miRNAs to Be Associated with Poor Prognosis in Chinese Patients
title_fullStr MicroRNA Expression Profile on Solid Subtype of Invasive Lung Adenocarcinoma Reveals a Panel of Four miRNAs to Be Associated with Poor Prognosis in Chinese Patients
title_full_unstemmed MicroRNA Expression Profile on Solid Subtype of Invasive Lung Adenocarcinoma Reveals a Panel of Four miRNAs to Be Associated with Poor Prognosis in Chinese Patients
title_short MicroRNA Expression Profile on Solid Subtype of Invasive Lung Adenocarcinoma Reveals a Panel of Four miRNAs to Be Associated with Poor Prognosis in Chinese Patients
title_sort microrna expression profile on solid subtype of invasive lung adenocarcinoma reveals a panel of four mirnas to be associated with poor prognosis in chinese patients
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039382/
https://www.ncbi.nlm.nih.gov/pubmed/27698898
http://dx.doi.org/10.7150/jca.14923
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