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Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease

Adult invasive disease caused by Group B Streptococcus (GBS) is increasing worldwide. Whole-genome sequencing (WGS) now permits rapid identification of recombination events, a phenomenon that occurs frequently in GBS. Using WGS, we described that strain NGBS375, a capsular serotype V GBS isolate of...

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Autores principales: Teatero, Sarah, Lemire, Paul, Dewar, Ken, Wasserscheid, Jessica, Calzas, Cynthia, Mallo, Gustavo V., Li, Aimin, Athey, Taryn B.T., Segura, Mariela, Fittipaldi, Nahuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039434/
https://www.ncbi.nlm.nih.gov/pubmed/27527222
http://dx.doi.org/10.3390/pathogens5030054
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author Teatero, Sarah
Lemire, Paul
Dewar, Ken
Wasserscheid, Jessica
Calzas, Cynthia
Mallo, Gustavo V.
Li, Aimin
Athey, Taryn B.T.
Segura, Mariela
Fittipaldi, Nahuel
author_facet Teatero, Sarah
Lemire, Paul
Dewar, Ken
Wasserscheid, Jessica
Calzas, Cynthia
Mallo, Gustavo V.
Li, Aimin
Athey, Taryn B.T.
Segura, Mariela
Fittipaldi, Nahuel
author_sort Teatero, Sarah
collection PubMed
description Adult invasive disease caused by Group B Streptococcus (GBS) is increasing worldwide. Whole-genome sequencing (WGS) now permits rapid identification of recombination events, a phenomenon that occurs frequently in GBS. Using WGS, we described that strain NGBS375, a capsular serotype V GBS isolate of sequence type (ST)297, has an ST1 genomic background but has acquired approximately 300 kbp of genetic material likely from an ST17 strain. Here, we examined the virulence of this strain in an in vivo model of GBS adult invasive infection. The mosaic ST297 strain showed intermediate virulence, causing significantly less systemic infection and reduced mortality than a more virulent, serotype V ST1 isolate. Bacteremia induced by the ST297 strain was similar to that induced by a serotype III ST17 strain, which was the least virulent under the conditions tested. Yet, under normalized bacteremia levels, the in vivo intrinsic capacity to induce the production of pro-inflammatory cytokines was similar between the ST297 strain and the virulent ST1 strain. Thus, the diminished virulence of the mosaic strain may be due to reduced capacity to disseminate or multiply in blood during a systemic infection which could be mediated by regulatory factors contained in the recombined region.
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spelling pubmed-50394342016-10-04 Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease Teatero, Sarah Lemire, Paul Dewar, Ken Wasserscheid, Jessica Calzas, Cynthia Mallo, Gustavo V. Li, Aimin Athey, Taryn B.T. Segura, Mariela Fittipaldi, Nahuel Pathogens Article Adult invasive disease caused by Group B Streptococcus (GBS) is increasing worldwide. Whole-genome sequencing (WGS) now permits rapid identification of recombination events, a phenomenon that occurs frequently in GBS. Using WGS, we described that strain NGBS375, a capsular serotype V GBS isolate of sequence type (ST)297, has an ST1 genomic background but has acquired approximately 300 kbp of genetic material likely from an ST17 strain. Here, we examined the virulence of this strain in an in vivo model of GBS adult invasive infection. The mosaic ST297 strain showed intermediate virulence, causing significantly less systemic infection and reduced mortality than a more virulent, serotype V ST1 isolate. Bacteremia induced by the ST297 strain was similar to that induced by a serotype III ST17 strain, which was the least virulent under the conditions tested. Yet, under normalized bacteremia levels, the in vivo intrinsic capacity to induce the production of pro-inflammatory cytokines was similar between the ST297 strain and the virulent ST1 strain. Thus, the diminished virulence of the mosaic strain may be due to reduced capacity to disseminate or multiply in blood during a systemic infection which could be mediated by regulatory factors contained in the recombined region. MDPI 2016-08-05 /pmc/articles/PMC5039434/ /pubmed/27527222 http://dx.doi.org/10.3390/pathogens5030054 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Teatero, Sarah
Lemire, Paul
Dewar, Ken
Wasserscheid, Jessica
Calzas, Cynthia
Mallo, Gustavo V.
Li, Aimin
Athey, Taryn B.T.
Segura, Mariela
Fittipaldi, Nahuel
Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease
title Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease
title_full Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease
title_fullStr Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease
title_full_unstemmed Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease
title_short Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease
title_sort genomic recombination leading to decreased virulence of group b streptococcus in a mouse model of adult invasive disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039434/
https://www.ncbi.nlm.nih.gov/pubmed/27527222
http://dx.doi.org/10.3390/pathogens5030054
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