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Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides

Biofilm matrices of two Klebsiella pneumoniae clinical isolates, KpTs101 and KpTs113, were investigated for their polysaccharide composition and protective effects against antimicrobial peptides. Both strains were good biofilm producers, with KpTs113 forming flocs with very low adhesive properties t...

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Autores principales: Benincasa, Monica, Lagatolla, Cristina, Dolzani, Lucilla, Milan, Annalisa, Pacor, Sabrina, Liut, Gianfranco, Tossi, Alessandro, Cescutti, Paola, Rizzo, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039586/
https://www.ncbi.nlm.nih.gov/pubmed/27681920
http://dx.doi.org/10.3390/microorganisms4030026
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author Benincasa, Monica
Lagatolla, Cristina
Dolzani, Lucilla
Milan, Annalisa
Pacor, Sabrina
Liut, Gianfranco
Tossi, Alessandro
Cescutti, Paola
Rizzo, Roberto
author_facet Benincasa, Monica
Lagatolla, Cristina
Dolzani, Lucilla
Milan, Annalisa
Pacor, Sabrina
Liut, Gianfranco
Tossi, Alessandro
Cescutti, Paola
Rizzo, Roberto
author_sort Benincasa, Monica
collection PubMed
description Biofilm matrices of two Klebsiella pneumoniae clinical isolates, KpTs101 and KpTs113, were investigated for their polysaccharide composition and protective effects against antimicrobial peptides. Both strains were good biofilm producers, with KpTs113 forming flocs with very low adhesive properties to supports. Matrix exopolysaccharides were isolated and their monosaccharide composition and glycosidic linkage types were defined. KpTs101 polysaccharide is neutral and composed only of galactose, in both pyranose and furanose ring configurations. Conversely, KpTs113 polysaccharide is anionic due to glucuronic acid units, and also contains glucose and mannose residues. The susceptibility of the two strains to two bovine cathelicidin antimicrobial peptides, BMAP-27 and Bac7(1–35), was assessed using both planktonic cultures and biofilms. Biofilm matrices exerted a relevant protection against both antimicrobials, which act with quite different mechanisms. Similar protection was also detected when antimicrobial peptides were tested against planktonic bacteria in the presence of the polysaccharides extracted from KpTs101 and KpTs113 biofilms, suggesting sequestering adduct formation with antimicrobials. Circular dichroism experiments on BMAP-27 in the presence of increasing amounts of either polysaccharide confirmed their ability to interact with the peptide and induce an α-helical conformation.
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spelling pubmed-50395862016-09-28 Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides Benincasa, Monica Lagatolla, Cristina Dolzani, Lucilla Milan, Annalisa Pacor, Sabrina Liut, Gianfranco Tossi, Alessandro Cescutti, Paola Rizzo, Roberto Microorganisms Article Biofilm matrices of two Klebsiella pneumoniae clinical isolates, KpTs101 and KpTs113, were investigated for their polysaccharide composition and protective effects against antimicrobial peptides. Both strains were good biofilm producers, with KpTs113 forming flocs with very low adhesive properties to supports. Matrix exopolysaccharides were isolated and their monosaccharide composition and glycosidic linkage types were defined. KpTs101 polysaccharide is neutral and composed only of galactose, in both pyranose and furanose ring configurations. Conversely, KpTs113 polysaccharide is anionic due to glucuronic acid units, and also contains glucose and mannose residues. The susceptibility of the two strains to two bovine cathelicidin antimicrobial peptides, BMAP-27 and Bac7(1–35), was assessed using both planktonic cultures and biofilms. Biofilm matrices exerted a relevant protection against both antimicrobials, which act with quite different mechanisms. Similar protection was also detected when antimicrobial peptides were tested against planktonic bacteria in the presence of the polysaccharides extracted from KpTs101 and KpTs113 biofilms, suggesting sequestering adduct formation with antimicrobials. Circular dichroism experiments on BMAP-27 in the presence of increasing amounts of either polysaccharide confirmed their ability to interact with the peptide and induce an α-helical conformation. MDPI 2016-08-10 /pmc/articles/PMC5039586/ /pubmed/27681920 http://dx.doi.org/10.3390/microorganisms4030026 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Benincasa, Monica
Lagatolla, Cristina
Dolzani, Lucilla
Milan, Annalisa
Pacor, Sabrina
Liut, Gianfranco
Tossi, Alessandro
Cescutti, Paola
Rizzo, Roberto
Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides
title Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides
title_full Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides
title_fullStr Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides
title_full_unstemmed Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides
title_short Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides
title_sort biofilms from klebsiella pneumoniae: matrix polysaccharide structure and interactions with antimicrobial peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039586/
https://www.ncbi.nlm.nih.gov/pubmed/27681920
http://dx.doi.org/10.3390/microorganisms4030026
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