Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens

Cancer cell heterogeneity is well-documented. Therefore, techniques to monitor single cell heterogeneous responses to treatment are needed. We developed a highly translational and quantitative bioluminescence microscopy method to measure single cell androgen receptor (AR) activity modulation by anti...

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Autores principales: Jain, Pallavi, Neveu, Bertrand, Velot, Lauriane, Wu, Lily, Fradet, Yves, Pouliot, Frédéric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039635/
https://www.ncbi.nlm.nih.gov/pubmed/27678181
http://dx.doi.org/10.1038/srep33968
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author Jain, Pallavi
Neveu, Bertrand
Velot, Lauriane
Wu, Lily
Fradet, Yves
Pouliot, Frédéric
author_facet Jain, Pallavi
Neveu, Bertrand
Velot, Lauriane
Wu, Lily
Fradet, Yves
Pouliot, Frédéric
author_sort Jain, Pallavi
collection PubMed
description Cancer cell heterogeneity is well-documented. Therefore, techniques to monitor single cell heterogeneous responses to treatment are needed. We developed a highly translational and quantitative bioluminescence microscopy method to measure single cell androgen receptor (AR) activity modulation by antiandrogens from fluid biopsies. We showed that this assay can detect heterogeneous cellular response to drug treatment and that the sum of single cell AR activity can mirror the response in the whole cell population. This method may thus be used to monitor heterogeneous dynamic treatment responses in cancer cells.
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spelling pubmed-50396352016-09-30 Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens Jain, Pallavi Neveu, Bertrand Velot, Lauriane Wu, Lily Fradet, Yves Pouliot, Frédéric Sci Rep Article Cancer cell heterogeneity is well-documented. Therefore, techniques to monitor single cell heterogeneous responses to treatment are needed. We developed a highly translational and quantitative bioluminescence microscopy method to measure single cell androgen receptor (AR) activity modulation by antiandrogens from fluid biopsies. We showed that this assay can detect heterogeneous cellular response to drug treatment and that the sum of single cell AR activity can mirror the response in the whole cell population. This method may thus be used to monitor heterogeneous dynamic treatment responses in cancer cells. Nature Publishing Group 2016-09-28 /pmc/articles/PMC5039635/ /pubmed/27678181 http://dx.doi.org/10.1038/srep33968 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jain, Pallavi
Neveu, Bertrand
Velot, Lauriane
Wu, Lily
Fradet, Yves
Pouliot, Frédéric
Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens
title Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens
title_full Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens
title_fullStr Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens
title_full_unstemmed Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens
title_short Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens
title_sort bioluminescence microscopy as a method to measure single cell androgen receptor activity heterogeneous responses to antiandrogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039635/
https://www.ncbi.nlm.nih.gov/pubmed/27678181
http://dx.doi.org/10.1038/srep33968
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