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Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds

A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glyc...

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Detalles Bibliográficos
Autores principales: Ahmad, Nor Monica, Abdullah, Jaafar, Yusof, Nor Azah, Ab Rashid, Ahmad Hazri, Abd Rahman, Samsulida, Hasan, Md. Rakibul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039650/
https://www.ncbi.nlm.nih.gov/pubmed/27367738
http://dx.doi.org/10.3390/bios6030031
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author Ahmad, Nor Monica
Abdullah, Jaafar
Yusof, Nor Azah
Ab Rashid, Ahmad Hazri
Abd Rahman, Samsulida
Hasan, Md. Rakibul
author_facet Ahmad, Nor Monica
Abdullah, Jaafar
Yusof, Nor Azah
Ab Rashid, Ahmad Hazri
Abd Rahman, Samsulida
Hasan, Md. Rakibul
author_sort Ahmad, Nor Monica
collection PubMed
description A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glycol (PEG), and zirconium oxide nanoparticles casted on screen printed carbon electrode (SPCE). Herein, the electrode was treated by casting hexacetyltrimethylammonium bromide on SPCE to promote a positively charged surface. Later, zirconium oxide was mixed with polyethylene glycol and the mixture was dropped cast onto the positively charged SPCE/CTAB. Tyrosinase was further immobilized onto the modified SPCE. Characterization of the prepared nanocomposite film and the modified SPCE surface was investigated by scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS), and Cyclic voltamogram (CV). The developed biosensor exhibits rapid response for less than 10 s. Two linear calibration curves towards phenol in the concentrations ranges of 0.075–10 µM and 10–55 µM with the detection limit of 0.034 µM were obtained. The biosensor shows high sensitivity and good storage stability for at least 30 days.
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spelling pubmed-50396502016-10-04 Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds Ahmad, Nor Monica Abdullah, Jaafar Yusof, Nor Azah Ab Rashid, Ahmad Hazri Abd Rahman, Samsulida Hasan, Md. Rakibul Biosensors (Basel) Article A phenolic biosensor based on a zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds has been explored. The formation of the composite film was expected via electrostatic interaction between hexacetyltrimethylammonium bromide (CTAB), polyethylene glycol (PEG), and zirconium oxide nanoparticles casted on screen printed carbon electrode (SPCE). Herein, the electrode was treated by casting hexacetyltrimethylammonium bromide on SPCE to promote a positively charged surface. Later, zirconium oxide was mixed with polyethylene glycol and the mixture was dropped cast onto the positively charged SPCE/CTAB. Tyrosinase was further immobilized onto the modified SPCE. Characterization of the prepared nanocomposite film and the modified SPCE surface was investigated by scanning electron microscopy (SEM), Electrochemical Impedance Spectroscopy (EIS), and Cyclic voltamogram (CV). The developed biosensor exhibits rapid response for less than 10 s. Two linear calibration curves towards phenol in the concentrations ranges of 0.075–10 µM and 10–55 µM with the detection limit of 0.034 µM were obtained. The biosensor shows high sensitivity and good storage stability for at least 30 days. MDPI 2016-06-29 /pmc/articles/PMC5039650/ /pubmed/27367738 http://dx.doi.org/10.3390/bios6030031 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahmad, Nor Monica
Abdullah, Jaafar
Yusof, Nor Azah
Ab Rashid, Ahmad Hazri
Abd Rahman, Samsulida
Hasan, Md. Rakibul
Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds
title Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds
title_full Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds
title_fullStr Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds
title_full_unstemmed Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds
title_short Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds
title_sort amperometric biosensor based on zirconium oxide/polyethylene glycol/tyrosinase composite film for the detection of phenolic compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039650/
https://www.ncbi.nlm.nih.gov/pubmed/27367738
http://dx.doi.org/10.3390/bios6030031
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