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MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing

Biomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either “evolution in the test tube” of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly im...

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Autores principales: Menger, Marcus, Yarman, Aysu, Erdőssy, Júlia, Yildiz, Huseyin Bekir, Gyurcsányi, Róbert E., Scheller, Frieder W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039654/
https://www.ncbi.nlm.nih.gov/pubmed/27438862
http://dx.doi.org/10.3390/bios6030035
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author Menger, Marcus
Yarman, Aysu
Erdőssy, Júlia
Yildiz, Huseyin Bekir
Gyurcsányi, Róbert E.
Scheller, Frieder W.
author_facet Menger, Marcus
Yarman, Aysu
Erdőssy, Júlia
Yildiz, Huseyin Bekir
Gyurcsányi, Róbert E.
Scheller, Frieder W.
author_sort Menger, Marcus
collection PubMed
description Biomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either “evolution in the test tube” of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly imprinted polymers (MIPs). The reproducible production of aptamers is a clear advantage, whilst the preparation of MIPs typically leads to a population of polymers with different binding sites. The realization of binding sites in the total bulk of the MIPs results in a higher binding capacity, however, on the expense of the accessibility and exchange rate. Furthermore, the readout of the bound analyte is easier for aptamers since the integration of signal generating labels is well established. On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the “biological” degradation of non-modified aptamers and ionic strength-dependent changes of conformation may be challenging in some application.
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spelling pubmed-50396542016-10-04 MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing Menger, Marcus Yarman, Aysu Erdőssy, Júlia Yildiz, Huseyin Bekir Gyurcsányi, Róbert E. Scheller, Frieder W. Biosensors (Basel) Review Biomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either “evolution in the test tube” of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly imprinted polymers (MIPs). The reproducible production of aptamers is a clear advantage, whilst the preparation of MIPs typically leads to a population of polymers with different binding sites. The realization of binding sites in the total bulk of the MIPs results in a higher binding capacity, however, on the expense of the accessibility and exchange rate. Furthermore, the readout of the bound analyte is easier for aptamers since the integration of signal generating labels is well established. On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the “biological” degradation of non-modified aptamers and ionic strength-dependent changes of conformation may be challenging in some application. MDPI 2016-07-18 /pmc/articles/PMC5039654/ /pubmed/27438862 http://dx.doi.org/10.3390/bios6030035 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Menger, Marcus
Yarman, Aysu
Erdőssy, Júlia
Yildiz, Huseyin Bekir
Gyurcsányi, Róbert E.
Scheller, Frieder W.
MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing
title MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing
title_full MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing
title_fullStr MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing
title_full_unstemmed MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing
title_short MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing
title_sort mips and aptamers for recognition of proteins in biomimetic sensing
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039654/
https://www.ncbi.nlm.nih.gov/pubmed/27438862
http://dx.doi.org/10.3390/bios6030035
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