Cargando…

MultiQC: summarize analysis results for multiple tools and samples in a single report

Motivation: Fast and accurate quality control is essential for studies involving next-generation sequencing data. Whilst numerous tools exist to quantify QC metrics, there is no common approach to flexibly integrate these across tools and large sample sets. Assessing analysis results across an entir...

Descripción completa

Detalles Bibliográficos
Autores principales: Ewels, Philip, Magnusson, Måns, Lundin, Sverker, Käller, Max
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039924/
https://www.ncbi.nlm.nih.gov/pubmed/27312411
http://dx.doi.org/10.1093/bioinformatics/btw354
_version_ 1782456150255468544
author Ewels, Philip
Magnusson, Måns
Lundin, Sverker
Käller, Max
author_facet Ewels, Philip
Magnusson, Måns
Lundin, Sverker
Käller, Max
author_sort Ewels, Philip
collection PubMed
description Motivation: Fast and accurate quality control is essential for studies involving next-generation sequencing data. Whilst numerous tools exist to quantify QC metrics, there is no common approach to flexibly integrate these across tools and large sample sets. Assessing analysis results across an entire project can be time consuming and error prone; batch effects and outlier samples can easily be missed in the early stages of analysis. Results: We present MultiQC, a tool to create a single report visualising output from multiple tools across many samples, enabling global trends and biases to be quickly identified. MultiQC can plot data from many common bioinformatics tools and is built to allow easy extension and customization. Availability and implementation: MultiQC is available with an GNU GPLv3 license on GitHub, the Python Package Index and Bioconda. Documentation and example reports are available at http://multiqc.info Contact: phil.ewels@scilifelab.se
format Online
Article
Text
id pubmed-5039924
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-50399242016-09-29 MultiQC: summarize analysis results for multiple tools and samples in a single report Ewels, Philip Magnusson, Måns Lundin, Sverker Käller, Max Bioinformatics Applications Notes Motivation: Fast and accurate quality control is essential for studies involving next-generation sequencing data. Whilst numerous tools exist to quantify QC metrics, there is no common approach to flexibly integrate these across tools and large sample sets. Assessing analysis results across an entire project can be time consuming and error prone; batch effects and outlier samples can easily be missed in the early stages of analysis. Results: We present MultiQC, a tool to create a single report visualising output from multiple tools across many samples, enabling global trends and biases to be quickly identified. MultiQC can plot data from many common bioinformatics tools and is built to allow easy extension and customization. Availability and implementation: MultiQC is available with an GNU GPLv3 license on GitHub, the Python Package Index and Bioconda. Documentation and example reports are available at http://multiqc.info Contact: phil.ewels@scilifelab.se Oxford University Press 2016-10-01 2016-06-16 /pmc/articles/PMC5039924/ /pubmed/27312411 http://dx.doi.org/10.1093/bioinformatics/btw354 Text en © The Author 2016. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Applications Notes
Ewels, Philip
Magnusson, Måns
Lundin, Sverker
Käller, Max
MultiQC: summarize analysis results for multiple tools and samples in a single report
title MultiQC: summarize analysis results for multiple tools and samples in a single report
title_full MultiQC: summarize analysis results for multiple tools and samples in a single report
title_fullStr MultiQC: summarize analysis results for multiple tools and samples in a single report
title_full_unstemmed MultiQC: summarize analysis results for multiple tools and samples in a single report
title_short MultiQC: summarize analysis results for multiple tools and samples in a single report
title_sort multiqc: summarize analysis results for multiple tools and samples in a single report
topic Applications Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039924/
https://www.ncbi.nlm.nih.gov/pubmed/27312411
http://dx.doi.org/10.1093/bioinformatics/btw354
work_keys_str_mv AT ewelsphilip multiqcsummarizeanalysisresultsformultipletoolsandsamplesinasinglereport
AT magnussonmans multiqcsummarizeanalysisresultsformultipletoolsandsamplesinasinglereport
AT lundinsverker multiqcsummarizeanalysisresultsformultipletoolsandsamplesinasinglereport
AT kallermax multiqcsummarizeanalysisresultsformultipletoolsandsamplesinasinglereport