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Ultra‐Specific Isolation of Circulating Tumor Cells Enables Rare‐Cell RNA Profiling

The clinical potential of circulating tumor cells (CTCs) in managing cancer metastasis is significant. However, low CTC isolation purities from patient blood have hindered sensitive molecular assays of these rare cells. Described herein is the ultra‐pure isolation of CTCs from patient blood samples...

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Autores principales: Jack, Rhonda M., Grafton, Meggie M. G., Rodrigues, Danika, Giraldez, Maria D., Griffith, Catherine, Cieslak, Robert, Zeinali, Mina, Kumar Sinha, Chandan, Azizi, Ebrahim, Wicha, Max, Tewari, Muneesh, Simeone, Diane M., Nagrath, Sunitha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039969/
https://www.ncbi.nlm.nih.gov/pubmed/27711257
http://dx.doi.org/10.1002/advs.201600063
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author Jack, Rhonda M.
Grafton, Meggie M. G.
Rodrigues, Danika
Giraldez, Maria D.
Griffith, Catherine
Cieslak, Robert
Zeinali, Mina
Kumar Sinha, Chandan
Azizi, Ebrahim
Wicha, Max
Tewari, Muneesh
Simeone, Diane M.
Nagrath, Sunitha
author_facet Jack, Rhonda M.
Grafton, Meggie M. G.
Rodrigues, Danika
Giraldez, Maria D.
Griffith, Catherine
Cieslak, Robert
Zeinali, Mina
Kumar Sinha, Chandan
Azizi, Ebrahim
Wicha, Max
Tewari, Muneesh
Simeone, Diane M.
Nagrath, Sunitha
author_sort Jack, Rhonda M.
collection PubMed
description The clinical potential of circulating tumor cells (CTCs) in managing cancer metastasis is significant. However, low CTC isolation purities from patient blood have hindered sensitive molecular assays of these rare cells. Described herein is the ultra‐pure isolation of CTCs from patient blood samples and how this platform has enabled highly specific molecular (mRNA and miRNA) profiling of patient CTCs. [Image: see text]
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spelling pubmed-50399692016-10-03 Ultra‐Specific Isolation of Circulating Tumor Cells Enables Rare‐Cell RNA Profiling Jack, Rhonda M. Grafton, Meggie M. G. Rodrigues, Danika Giraldez, Maria D. Griffith, Catherine Cieslak, Robert Zeinali, Mina Kumar Sinha, Chandan Azizi, Ebrahim Wicha, Max Tewari, Muneesh Simeone, Diane M. Nagrath, Sunitha Adv Sci (Weinh) Communications The clinical potential of circulating tumor cells (CTCs) in managing cancer metastasis is significant. However, low CTC isolation purities from patient blood have hindered sensitive molecular assays of these rare cells. Described herein is the ultra‐pure isolation of CTCs from patient blood samples and how this platform has enabled highly specific molecular (mRNA and miRNA) profiling of patient CTCs. [Image: see text] John Wiley and Sons Inc. 2016-04-18 /pmc/articles/PMC5039969/ /pubmed/27711257 http://dx.doi.org/10.1002/advs.201600063 Text en © 2016 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Jack, Rhonda M.
Grafton, Meggie M. G.
Rodrigues, Danika
Giraldez, Maria D.
Griffith, Catherine
Cieslak, Robert
Zeinali, Mina
Kumar Sinha, Chandan
Azizi, Ebrahim
Wicha, Max
Tewari, Muneesh
Simeone, Diane M.
Nagrath, Sunitha
Ultra‐Specific Isolation of Circulating Tumor Cells Enables Rare‐Cell RNA Profiling
title Ultra‐Specific Isolation of Circulating Tumor Cells Enables Rare‐Cell RNA Profiling
title_full Ultra‐Specific Isolation of Circulating Tumor Cells Enables Rare‐Cell RNA Profiling
title_fullStr Ultra‐Specific Isolation of Circulating Tumor Cells Enables Rare‐Cell RNA Profiling
title_full_unstemmed Ultra‐Specific Isolation of Circulating Tumor Cells Enables Rare‐Cell RNA Profiling
title_short Ultra‐Specific Isolation of Circulating Tumor Cells Enables Rare‐Cell RNA Profiling
title_sort ultra‐specific isolation of circulating tumor cells enables rare‐cell rna profiling
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039969/
https://www.ncbi.nlm.nih.gov/pubmed/27711257
http://dx.doi.org/10.1002/advs.201600063
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